Histological evaluation of the regenerative potential of a novel photocrosslinkable gelatin-treated dentin matrix hydrogel in direct pulp capping: an animal study.

BACKGROUND: To assess histologically the success of the pulp capping approach performed in traumatically exposed dogs' teeth using a novel injectable gelatin-treated dentin matrix light cured hydrogel (LCG-TDM) compared with LCG, MTA and TheraCal LC. METHODS: Sixty-four dogs' teeth were divided into two groups (each including 32 teeth) based on the post-treatment evaluation period: group I: 2 weeks and group II: 8 weeks. Each group was further subdivided according to the pulp capping material into four subgroups (n = 8), with subgroup A (light-cured gelatin hydrogel) as the control subgroup, subgroup B (LCG-TDM), subgroup C (TheraCal LC), and subgroup D (MTA). Pulps were mechanically exposed in the middle of the cavity floor and capped with different materials. An assessment of periapical response was performed preoperatively and at 8 weeks. After 2 and 8-week intervals, the dogs were sacrificed, and the teeth were stained with hematoxylin-eosin and graded by using a histologic scoring system. Statistical analysis was performed using the chi-square and Kruskal-Wallis tests (p = 0.05). RESULTS: All subgroups showed mild inflammation with normal pulp tissue at 2 weeks with no significant differences between subgroups (p ≤ 0.05), except for the TheraCal LC subgroup, which exhibited moderate inflammation (62.5%). Absence of a complete calcified bridge was reported in all subgroups at 2 weeks, while at 8 weeks, the majority of samples in the LCG-TDM and MTA-Angelus subgroups showed complete dentin bridge formation and absence of inflammatory pulp response with no significant differences between them (p ≤ 0.05). However, the formed dentin in the LCG-TDM group was significantly thicker, with layers of ordered odontoblasts identified to create a homogeneous tubular structure and numerous dentinal tubule lines suggesting a favourable trend towards dentin regeneration. TheraCal LC samples revealed a reasonably thick dentin bridge with moderate inflammation (50%) and LCG showed heavily fibrous tissue infiltrates with areas of degenerated pulp with no signs of hard tissue formation. CONCLUSIONS: LCG-TDM, as an extracellular matrix-based material, has the potential to regenerate dentin and preserve pulp vitality, making it a viable natural alternative to silicate-based cements for healing in vivo dentin defects in direct pulp-capping procedures.

Three-dimensionally printed denture base resins modified by nanoglass particles and carbon nanotubes.

STATEMENT OF PROBLEM: Three-dimensionally (3D) printed denture base resins exhibit inferior mechanical properties compared with conventional and milled ones, a problem affecting their long-term clinical use. Improved 3D printed resins are required. PURPOSE: The purpose of this in vitro study was to determine whether a 3D printed denture base resin with nanoglass particles and multiwalled carbon nanotubes (MWCNTs) would exhibit enhanced mechanical properties. MATERIAL AND METHODS: The nanoglass particles and MWCNTs were silane coated and added to the resin to obtain the following groups: Control, resin modified with nanoglass particles with 2 percentages, 0.25 wt%, and 0.5 wt%; resin modified with MWCNTs with 2 percentages, 0.25 wt%, and 0.5 wt%; and a combination group with 0.25 wt% of each filler type. The printed specimens (N=330) were tested before and after thermocycling (600 cycles) for flexural strength (FS) and elastic modulus (n=22) by using a universal testing machine and for impact strength (IS) (n=22) by using a Charpy impact tester. The fractured impact specimens were then evaluated by using scanning electron microscopy (SEM). The surface roughness (Ra) (n=11) was assessed by using a profilometer. For data analysis, the 2-way ANOVA test was used for the analysis of FS, elastic modulus, and IS, and the 3-way ANOVA test was used for Ra with a subsequent Tukey post hoc test. Percentage change was compared among groups by using the Kruskal-Wallis test, followed by the Dunn post hoc test with Bonferroni correction (α=.05). RESULTS: The filler content and thermocycling revealed a significant main interaction effect (P<.001) on FS, elastic modulus, and IS, with the 0.5% nanoglass group displaying the highest percentage decrease after thermocycling. The SEM images of fractured impact specimens revealed a brittle failure in the control and nanoglass groups. In contrast, the groups containing MWCNTs and the combination group displayed intermediate to ductile failure. Moreover, a significant inclusive interaction effect (P<.001) was found between the filler content, thermocycling, and polishing on surface roughness, with the 0.5% nanoglass group revealing the highest percentage increase in Ra of the polished surface after aging. CONCLUSIONS: The addition of nanoglass and MWCNTs led to a significant improvement in the FS, elastic modulus, and IS of the 3D printed resin. The combination group displayed the least percentage change among all groups regarding the FS and IS, displaying intermediate to ductile failure. The control revealed the least percentage change in elastic modulus after thermocycling but with lower peak values compared with all other groups.

Photocrosslinkable gelatin-treated dentin matrix hydrogel as a novel pulp capping agent for dentin regeneration: I. synthesis, characterizations and grafting optimization.

BACKGROUND: In recent years, treated dentin matrix (TDM) has been introduced as a bioactive hydrogel for dentin regeneration in DPC. However, no study has introduced TDM as a photocrosslinkable hydrogel with a natural photoinitiating system. Therefore, the present study aimed to explore the synthesis, characterizations and grafting optimization of injectable gelatin- glycidyl methacrylate (GMA)/TDM hydrogels as a novel photocrosslinkable pulp capping agent for dentin regeneration. METHODS: G-GMA/TDM hydrogel was photocrosslinked using a new two-component photoinitiating system composed of riboflavin as a photoinitiator under visible light and glycine as a first time coinitiator with riboflavin. The grafting reaction conditions of G-GMA/TDM e.g. GMA concentration and reaction time were optimized. The kinetic parameters e.g. grafting efficiency (GE) and grafting percentage (GP%) were calculated to optimize the grafting reaction, while yield (%) was determined to monitor the formation of the hydrogel. Moreover, G-GMA/TDM hydrogels were characterized by swelling ratio, degradation degree, and cytotoxicity. The instrumental characterizations e.g. FTIR, 1H-NMR, SEM and TGA, were investigated for verifying the grafting reaction. Statistical analysis was performed using F test (ANOVA) and Post Hoc Test (P = 0.05). RESULTS: The grafting reaction dramatically increased with an increase of both GMA concentration and reaction time. It was realized that the swelling degree and degradation rate of G-GMA/TDM hydrogels were significantly reduced by increasing the GMA concentration and prolonging the reaction time. When compared to the safe low and moderate GMA content hydrogels (0.048, 0.097 M) and shorter reaction times (6, 12, 24 h), G-GMA/TDM with high GMA contents (0.195, 0.391 M) and a prolonged reaction time (48 h) demonstrated cytotoxic effects against cells using the MTT assay. Also, the morphological surface of G-GMA/TDM freeze-dried gels was found more compacted, smooth and uniform due to the grafting process. Significant thermal stability was noticed due to the grafting reaction of G-GMA/TDM throughout the TGA results. CONCLUSIONS: G-GMA/TDM composite hydrogel formed by the riboflavin/glycine photoinitiating system is a potential bioactive and biocompatible system for in-situ crosslinking the activated-light pulp capping agent for dentin regeneration.

The effect of sandblasting versus acid etching on the surface roughness and biaxial flexural strength of CAD/CAM resin-matrix ceramics (In vitro study).

BACKGROUND: CAD/CAM resin matrix ceramics are one of the materials used in dental offices. The long-term success of the restoration depends on the bond strength of the restoration to the tooth and other materials; thus, surface treatment of the restoration is necessary to achieve this. But such treatment may affect the restoration strength. The purpose of this study is to assess the impact of various surface treatments on the surface roughness (Ra) and the biaxial flexural strength of two CAD-CAM resin-matrix ceramics. METHODS: Thirty-six-disc-shaped specimens, each measuring 1.2 mm in thickness and 12 mm in diameter, were machined from two resin-matrix ceramic blocks (Lava Ultimate and Cerasmart) (n = 18). Based on the surface treatments, each material was divided into 3 groups: control (no treatment), 50-µm Al2O3 sandblasting, or 9% hydrofluoric acid etching (n = 6). The surface roughness (Ra) was evaluated by the 3D laser scanning microscope. Then, specimens were aged by thermal cycling (5000 cycles) and tested for biaxial flexural strength using a universal testing machine at a crosshead speed of 1.0 mm/min. RESULTS: No significant differences in flexural strength or Ra were found for Lava Ultimate among the surface treatment groups. For Cerasmart, only the sandblasting group showed significantly higher Ra values than the control group. Also, the Ra values for the sandblasting group were significantly higher than those for the acid etching group. The flexural strengths of the sandblasting and acid etching groups for Cerasmart were statistically similar, and both were significantly lower than the control group. CONCLUSIONS: Although all of the applied surface conditioning techniques improved Ra, they had a negative impact on the flexural strength of resin-matrix ceramics. Thus, clinicians should utilize the appropriate surface treatment techniques, taking into account their effects on the surface roughness and mechanical properties of resin-matrix ceramics.

Improved Mechanical Properties and Bioactivity of Silicate Based Bioceramics Reinforced Poly(ether-ether-ketone) Nanocomposites for Prosthetic Dental Implantology.

Polyether-ether-ketone (PEEK) biomaterial has been increasingly employed for orthopedic, trauma, spinal, and dental implants due to its biocompatibility and in vivo stability. However, a lack of bioactivity and binding ability to natural bone tissue has significantly limited PEEK for many challenging dental implant applications. In this work, nanocomposites based on PEEK reinforced with bioactive silicate-based bioceramics (forsterite or bioglass) as nanofillers were prepared using high energy ball milling followed by melt blending and compression molding. The influence of nanofillers type and content (10, 20 and 30 wt.%) on the crystalline structure, morphology, surface roughness, hydrophilicity, microhardness, elastic compression modulus, and flexural strength of the nanocomposites was investigated. The scanning electron microscopy images of the nanocomposites with low nanofillers content showed a homogenous surface with uniform dispersion within the PEEK matrix with no agglomerates. All nanocomposites showed an increased surface roughness compared to pristine PEEK. It was found that the incorporation of 20 wt.% forsterite was the most effective in the nanocomposite formulation compared with bioglass-based nanocomposites; it has significantly improved the elastic modulus, flexural strength, and microhardness. In vitro bioactivity evaluation, which used biomimetic simulated body fluid indicated the ability of PEEK nanocomposites loaded with forsterite or bioglass nanofillers to precipitate calcium and phosphate bone minerals on its surface. These nanocomposites are expected to be used in long-term load-bearing implant applications and could be recommended as a promising alternative to titanium and zirconia when used as a dental implant material.

Effect of surface treated silicon dioxide nanoparticles on some mechanical properties of maxillofacial silicone elastomer.

Current materials used for maxillofacial prostheses are far from ideal and there is a need for novel improved materials which mimic as close as possible the natural behavior of facial soft tissues. This study aimed to evaluate the effect of adding different concentrations of surface treated silicon dioxide nanoparticles (SiO2) on clinically important mechanical properties of a maxillofacial silicone elastomer. 147 specimens of the silicone elastomer were prepared and divided into seven groups (n = 21). One control group was prepared without nanoparticles and six study groups with different concentrations of nanoparticles, from 0.5% to 3% by weight. Specimens were tested for tear strength (ASTM D624), tensile strength (ASTM D412), percent elongation, and shore A hardness. SEM was used to assess the dispersion of nano-SiO2 within the elastomer matrix. Data were analyzed by one-way ANOVA and Scheffe test (α = 0.05). Results revealed significant improvement in all mechanical properties tested, as the concentration of the nanoparticles increased. This was supported by the results of the SEM. Hence, it can be concluded that the incorporation of surface treated SiO2 nanoparticles at concentration of 3% enhanced the overall mechanical properties of A-2186 silicone elastomer.