1.
J Assoc Off Anal Chem
; 70(2): 201-5, 1987.
Article
in English
| MEDLINE
| ID: mdl-3571111
ABSTRACT
Monensin is converted to monensin bromoacetate, which provides successful coupling to protein and allows production of monensin-specific rabbit antisera. A modified indirect enzyme-linked immunosorbent assay (ELISA) was developed, which is highly sensitive (2 ng/mL) to monensin determination. Monensin recovery was 53-81% at 10 ppb in sera or urine, and 81-130% at 100 ppb in dichloromethane-extracted feces or water. Overall recovery was 98.8% with coefficients of variation from 6 to 52% over the range of monensin concentrations studied. This is the first immunoassay reported for the carboxylic ionophore antibiotics.
Subject(s)
Enzyme-Linked Immunosorbent Assay , Monensin/analysis , Animals , Antibody Formation , Antibody Specificity , Cattle , Cross Reactions , Horses/immunology , Humans , Indicators and Reagents , Monensin/immunology , Proteins/analysis
2.
J Med Chem
; 12(3): 420-4, 1969 May.
Article
in English
| MEDLINE
| ID: mdl-5788157