ABSTRACT
Affinity peptide and protein- (APP) based radiotracers are an increasingly popular class of radiotracer in positron emission tomography (PET), which was once dominated by the use of small molecule radiotracers. Radiolabelled monoclonal antibodies (mAbs) are important examples of APPs, yet a preference for smaller APPs, which exhibit fast pharmacokinetics and permit rapid PET aided diagnosis, has become apparent. 18 F exhibits favourable physical characteristics for APP radiolabelling and has been described as an ideal PET radionuclide. Notwithstanding, 18 F radiolabelling of APP is challenging, and this is echoed in the literature where a number of diverse approaches have been adopted. This review seeks to assess and compare the approaches taken to 18 F APP radiolabelling with the intention of highlighting trends within this expanding field. Generic themes have emerged in the literature, namely the use of mild radiolabelling conditions, a preference of site-specific methodologies with an impetus for short, automated procedures which produce high-yielding [18 F]APPs.
Subject(s)
Fluorine Radioisotopes/chemistry , Peptides/chemistry , Positron-Emission Tomography/methods , Radiopharmaceuticals/chemical synthesis , Antibodies, Monoclonal/chemistry , Automation/methods , Chemistry Techniques, Synthetic/methods , Humans , Radiopharmaceuticals/pharmacokineticsABSTRACT
Positron Emission Tomography is an attractive imaging modality for monitoring the migration of cells to pathological tissue. We evaluated a new method for radiolabelling leukocytes with zirconium-89 (89Zr) using chitosan nanoparticles (CN, Z-average size 343 ± 210nm and zeta potential +46 ± 4mV) as the carrier. We propose that cell uptake of 89Zr-loaded CN occurred in a two-step process; cell membrane interaction with 89Zr-loaded CN was followed by a slower cell internalisation step.
Subject(s)
Chemotaxis, Leukocyte , Chitosan/chemistry , Leukocytes/cytology , Nanoparticles/chemistry , Positron-Emission Tomography/methods , Radioisotopes/chemistry , Zirconium/chemistry , Cell Separation , Flow Cytometry , HumansABSTRACT
Positron emission tomography (PET) and fluorescence labelling have been used to assess the pharmacokinetics, biodistribution and eventual fate of a hydrogel-forming nonapeptide, FEFKFEFKK (F9), in healthy mice, using 18 F-labelled and fluorescein isothiocyanate (FITC)-labelled F9 analogues. F9 was site-specifically radiolabelled with 2-[18 F]fluoro-3-pyridinecarboxaldehyde ([18 F]FPCA) via oxime bond formation. [18 F]FPCA-F9 in vivo fate was evaluated both as a solution, following intravenous administration, and as a hydrogel when subcutaneously injected. The behaviour of FITC-F9 hydrogel was assessed following subcutaneous injection. [18 F]FPCA-F9 demonstrated high plasma stability and primarily renal excretion; [18 F]FPCA-F9 when in solution and injected into the bloodstream displayed prompt bladder uptake (53.4 ± 16.6 SUV at 20 minutes postinjection) and rapid renal excretion, whereas [18 F]FPCA-F9 hydrogel, formed by co-assembly of [18 F]FPCA-F9 monomer with unfunctionalised F9 peptide and injected subcutaneously, showed gradual bladder accumulation of hydrogel fragments (3.8 ± 0.4 SUV at 20 minutes postinjection), resulting in slower renal excretion. Gradual disaggregation of the F9 hydrogel from the site of injection was monitored using FITC-F9 hydrogel in healthy mice (60 ± 3 over 96 hours), indicating a biological half-life between 1 and 4 days. The in vivo characterisation of F9, both as a gel and a solution, highlights its potential as a biomaterial.
Subject(s)
Fluorine Radioisotopes/therapeutic use , Hydrogels/chemistry , Oligopeptides/chemistry , Oligopeptides/therapeutic use , Positron-Emission Tomography , Amino Acid Sequence , Animals , Drug Stability , Half-Life , Mice , Oligopeptides/metabolism , Oligopeptides/pharmacokinetics , Protein Conformation, beta-Strand , Tissue DistributionABSTRACT
Mixed leukocyte (white blood cells [WBCs]) trafficking using positron emission tomography (PET) is receiving growing interest to diagnose and monitor inflammatory conditions. PET, a high sensitivity molecular imaging technique, allows precise quantification of the signal produced from radiolabelled moieties. We have evaluated a new method for radiolabelling WBCs with either zirconium-89 ((89) Zr) or copper-64 ((64) Cu) for PET imaging. Chitosan nanoparticles (CNs) were produced by a process of ionotropic gelation and used to deliver radiometals into WBCs. Experiments were carried out using mixed WBCs freshly isolated from whole human blood. WBCs radiolabelling efficiency was higher with [(89) Zr]-loaded CN (76.8 ± 9.6% (n = 12)) than with [(64) Cu]-loaded CN (26.3 ± 7.0 % (n = 7)). [(89) Zr]-WBCs showed an initial loss of 28.4 ± 5.8% (n = 2) of the radioactivity after 2 h. This loss was then followed by a plateau as (89) Zr remains stable in the cells. [(64) Cu]-WBCs showed a loss of 85 ± 6% (n = 3) of the radioactivity after 1 h, which increased to 96 ± 6% (n = 3) loss after 3 h. WBC labelling with [(89) Zr]-loaded CN showed a fast kinetic of leukocyte association, high labelling efficiency and a relatively good retention of the radioactivity. This method using (89) Zr has a potential application for PET imaging of inflammation.
Subject(s)
Isotope Labeling/methods , Leukocytes/metabolism , Positron-Emission Tomography , Radioisotopes , Zirconium , Chitosan/chemistry , Copper Radioisotopes , Deferoxamine/chemistry , Humans , Inflammation/diagnostic imaging , Nanoparticles/chemistryABSTRACT
IL-1RA is a naturally occurring antagonist of the pro-inflammatory cytokine interleukin-1 (IL-1) with high therapeutic promise, but its pharmacokinetic remains poorly documented. In this report, we describe the radiolabeling of recombinant human interleukin-1 receptor antagonist (rhIL-1RA) with fluorine-18 to allow pharmacokinetic studies by positron emission tomography (PET). rhIL-1RA was labeled randomly by reductive alkylation of free amino groups (the epsilon-amino group of lysine residues or amino-terminal residues) using [(18)F]fluoroacetaldehyde under mild reaction conditions. Radiosyntheses used a remotely controlled experimental rig within 100min and the radiochemical yield was in the range 7.1-24.2% (decay corrected, based on seventeen syntheses). We showed that the produced [(18)F]fluoroethyl-rhIL-1ra retained binding specificity by conducting an assay on rat brain sections, allowing its pharmakokinetic study using PET.
Subject(s)
Brain/diagnostic imaging , Brain/metabolism , Fluorine Radioisotopes/pharmacokinetics , Positron-Emission Tomography/methods , Receptors, Interleukin-1/antagonists & inhibitors , Receptors, Interleukin-1/metabolism , Animals , Fluorine Radioisotopes/chemistry , Isotope Labeling/methods , Male , Radiopharmaceuticals/chemical synthesis , Radiopharmaceuticals/pharmacokinetics , Rats , Rats, Sprague-Dawley , Tissue DistributionABSTRACT
We developed a visual field simulator for teaching perimetry on a Goldmann-type perimeter. It can present reproducible normal and defective visual fields, is portable and can be used without being attached to the perimeter, and will provide an easier way to train individuals in the correct techniques of perimetry.
Subject(s)
Ophthalmology/education , Visual Field Tests/instrumentation , Visual Fields , Humans , Ophthalmology/instrumentation , Teaching Materials/standardsABSTRACT
This study shows that the field defects in simple glaucoma can occur as paracentral scotoma (77%) as well as nasal skip defects (20%) in the central or peripheral field. Also a combination (54%) of both defects is already possible at the start. This points out that more than one nerve bundle can be affected either simultaneously or one shortly after the other. It was also remarkable that by Drance's modification of Armaly's method for two eyes a typical nasal skip and/or a paracentral scotoma with temporal visual field defect occurred as the first sign.
Subject(s)
Glaucoma/complications , Vision Disorders/etiology , Humans , Scotoma/etiology , Visual Field Tests , Visual FieldsABSTRACT
The progression of field damage of 48 eyes with chronic simple glaucoma and a splinter hemorrhage on the disc was compared with that of 48 chronic simple glaucoma eyes without hemorrhage. The incidence of visual field progression was substantially higher among those with a hemorrhage. Similar visual field progression was substantially higher in 29 eyes with elevated introcular pressure and a disc hemorrhage when compared with 29 ocular hypertensive patients with no hemorrhage. The prognostic importance of a disc hemorrhage is discussed.