ABSTRACT
We have probed the surface accessibility of residues alpha187 to alpha199 of the Torpedo acetylcholine receptor with monoclonal antibody 383C, which binds uniquely to these residues. However, 383C binds to only one of the two alpha subunits in the membrane-bound receptor, neither of the two subunits in carbamylcholine-desensitized receptor, and to both alpha subunits in Triton X-100 solubilized receptor. The kinetics of association and dissoci-ation of 383C with the peptide alpha(183-199) compared to those with the membrane-bound receptor suggest that all but a single hydrogen bond of affinity derives from contacts between this peptide and the monoclonal antibody paratope. Inhibition of 383C binding by alpha-bungarotoxin selectively directed to the alpha subunit correlated with the high-affinity d-tubocurarine binding site, along with a lack of inhibition by alpha-bungarotoxin directed to the alpha subunit correlated with the low-affinity d-tubocurarine binding site, suggests that the 383C epitope on the membrane-bound receptor resides on the alpha subunit associated with the high-affinity d-tubocurarine binding site. The results presented here suggest a structural basis for the differences between the two receptor acetylcholine binding sites.
Subject(s)
Epitopes/analysis , Receptors, Nicotinic/chemistry , Animals , Antibodies, Monoclonal/metabolism , Binding Sites , Bungarotoxins/metabolism , Carbachol/metabolism , Kinetics , Membrane Proteins/chemistry , Membrane Proteins/metabolism , Octoxynol/metabolism , Receptors, Nicotinic/metabolism , Titrimetry , Torpedo/physiology , Tubocurarine/metabolismABSTRACT
Monoclonal antibody 383C is an anti-acetylcholine receptor antibody whose binding to the receptor is blocked by alpha-bungarotoxin and by carbamylcholine. Monoclonal antibody 383C binds to the alpha subunit of the Torpedo acetylcholine (ACh) receptor as well as to its V8-protease 20 kDa fragment that possesses the affinity alkylatable Cys192/193. In an epitope scanning experiment spanning the N-terminal 211 amino acid residues of the alpha subunit, 383C binds uniquely to three overlapping peptides; alpha(184-196), alpha(187-199) and alpha(190-202). These peptides span a cluster of amino acid residues implicated in the binding of acetylcholine, including Cys192/193. To map the location of these residues on the three-dimensional model of the ACh receptor, we have employed a combination of X-ray diffraction from oriented complexes of 383C with ACh receptor-enriched membrane vesicles and electron microscopy of negatively stained tubular arrays of 383C/receptor complexes. The X-ray diffraction study finds extra electron density in the presence of 383C centered 35 A above the synaptic side phosphate head groups. The electron micrographic images display extra stain exclusion from the antibody at a site adjacent to the alpha2 subunit on the periphery of the rosette clockwise to the alpha2 vertex. This mapping localizes several residues of the ACh receptor alpha subunit involved in the binding of acetylcholine. Despite these residues being present in both alpha subunits, only the alpha2 subunit is decorated with this monoclonal antibody.
Subject(s)
Epitope Mapping/methods , Epitopes/analysis , Models, Molecular , Receptors, Cholinergic/chemistry , Amino Acid Sequence , Animals , Antibodies, Monoclonal , Crystallography, X-Ray , Epitopes/metabolism , Lipid Bilayers/chemistry , Receptor Aggregation , TorpedoSubject(s)
Antibodies, Monoclonal/pharmacology , Epitopes/immunology , Ion Channels/physiology , Receptors, Cholinergic/physiology , Animals , Carbachol/pharmacology , Cholinergic Agonists/pharmacology , Epitopes/chemistry , Ion Channel Gating/drug effects , Ion Channel Gating/physiology , Ion Channels/immunology , Kinetics , Models, Molecular , Peptide Fragments/chemistry , Peptide Fragments/immunology , Receptors, Cholinergic/chemistry , Receptors, Cholinergic/immunologySubject(s)
Antibodies, Monoclonal , Receptors, Cholinergic/metabolism , Tubocurarine/metabolism , Animals , Binding Sites , Bungarotoxins/metabolism , Cell Membrane/metabolism , Enzyme-Linked Immunosorbent Assay , Kinetics , Macromolecular Substances , Receptors, Cholinergic/analysis , Receptors, Cholinergic/immunology , TorpedoSubject(s)
Epitopes/immunology , Myasthenia Gravis/immunology , Receptors, Cholinergic/immunology , Animals , Antibodies, Monoclonal , Antigen-Presenting Cells/immunology , B-Lymphocytes/immunology , Female , Peptide Fragments/immunology , Rats , Rats, Inbred Lew , Recombinant Fusion Proteins/immunologySubject(s)
Autoantibodies/blood , Chorea/immunology , Muscle Proteins/immunology , Myasthenia Gravis/immunology , Receptors, Cholinergic/immunology , Receptors, Nicotinic/immunology , Animals , Cell Membrane/immunology , Chorea/blood , Electric Organ/chemistry , Female , Humans , Muscle Proteins/isolation & purification , Myasthenia Gravis/blood , Myotonia/immunology , Rats , Rats, Inbred Lew , Receptors, Cholinergic/isolation & purification , Receptors, Nicotinic/isolation & purification , Reference Values , Synapses/immunology , Tissue Extracts , TorpedoSubject(s)
Autoantibodies/blood , Chorea/immunology , Dystrophin-Associated Proteins , Muscle Proteins/immunology , Protein Kinases/immunology , Receptors, Cholinergic/immunology , Synapses/ultrastructure , Animals , Chorea/blood , Chorea/physiopathology , Connectin , Electric Organ/chemistry , Humans , Male , Membrane Proteins/immunology , Middle Aged , Muscle, Skeletal/immunology , Neuropeptides/immunology , Receptors, Nicotinic/immunology , TorpedoSubject(s)
Autoantibodies/blood , Muscle Proteins/immunology , Neuromuscular Diseases/chemically induced , Neuromuscular Diseases/immunology , Procainamide/adverse effects , Receptors, Nicotinic/immunology , Aged , Aged, 80 and over , Animals , Chronic Disease , Electric Organ/immunology , Enzyme-Linked Immunosorbent Assay , Humans , Male , Neuromuscular Diseases/blood , TorpedoSubject(s)
Receptors, Nicotinic/chemistry , Amino Acid Sequence , Animals , Antibodies, Monoclonal/immunology , Binding Sites , Epitopes , Image Processing, Computer-Assisted , In Vitro Techniques , Ion Channel Gating , Ligands , Macromolecular Substances , Microscopy, Electron , Molecular Sequence Data , Receptors, Nicotinic/metabolism , Receptors, Nicotinic/ultrastructure , Scattering, Radiation , Structure-Activity Relationship , TorpedoSubject(s)
Penicillamine/pharmacology , Receptors, Nicotinic/immunology , Animals , Antibodies, Monoclonal/immunology , Antigen-Antibody Reactions/drug effects , Cysteine/chemistry , Epitopes , In Vitro Techniques , Iodoacetamide/chemistry , Penicillamine/chemistry , Receptors, Nicotinic/chemistry , TorpedoABSTRACT
To analyze components of the idiotypic network in experimental autoimmune disease, we produced 17 isogeneic anti-idiotopic monoclonal antibodies (anti-Id) against two experimental autoimmune myasthenia gravis-producing anti-acetylcholine receptor (anti-AChR) monoclonal antibodies. We studied the binding of five of the anti-Id to the anti-AChR monoclonal antibodies bearing the complementary idiotopes (Id-mAb). They bound with Kd values ranging from 0.06 to 0.86 nM, values comparable to those of Id-mAb:AChR complexes (0.26 and 0.34 nM). All of the anti-Id tested moderately inhibited the binding of AChR to Id-mAb, whereas for each anti-Id, AChR either strongly inhibited anti-Id binding or had no effect on anti-Id binding. Hence, the inhibition of Id-mAb:AChR binding by anti-Id was not reciprocal with the inhibition of anti-Id:Id-mAb binding by AChR. For each anti-Id, the relative affinities of anti-Id and AChR for Id-mAb together with the lack of symmetry of inhibition by anti-Id compared to inhibition by AChR indicate that these two "ligands" are not competitive inhibitors. Consequently, anti-Id and AChR do not bind to overlapping sites on the Id-mAb, suggesting that the observed inhibition is mediated allosterically. This may be a common mechanism of anti-Id:Id binding, which would have important implications for the mechanism of anti-Id-induced suppression.