ABSTRACT
AIMS: We examined the possible association of the -308G/A polymorphism of the TNF-α promoter gene in type 2 diabetes mellitus (DM2) patients and in non-diabetic subjects with and without family history of DM2. METHODS: We studied 87 non-diabetic subjects without DM2 family history in at least one of two generations, 48 non-diabetic subjects with DM2 family history and 95 DM2 patients. Genotyping was carried out by PCR-RFLP. RESULTS: The frequency of TNF-α -308G/A genotype was significantly lower in non-diabetic subjects without DM2 relatives (6%) as compared to DM2 patients (24%) (odds ratio (OR)=5.24; 95% confidence interval (CI)=1.9-15.8, p<0.0005), but similar to non-diabetic subjects with DM2 relatives (29%) (OR=0.77; CI=0.3-1.7, p=0.4). Logistic regression analysis showed the association of TNF-α -308G/A polymorphism with DM2 family history (OR=5.80; CI=1.77-18.98, p<0.0003). CONCLUSIONS: Our results suggest that TNF-α -308G/A polymorphism is associated with DM2 family history and is a risk factor for DM2.
Subject(s)
Diabetes Mellitus, Type 2/genetics , Polymorphism, Genetic , Tumor Necrosis Factor-alpha/blood , Tumor Necrosis Factor-alpha/genetics , Adult , Aged , Cross-Sectional Studies , Diabetes Mellitus, Type 2/blood , Female , Genotype , Humans , Insulin Resistance , Male , Mexico , Middle Aged , Odds Ratio , Polymorphism, Restriction Fragment LengthABSTRACT
AIM: We studied the effect of atorvastatin (ATV) treatment on bone loss prevention in subjects with chronic periodontitis. MATERIAL AND METHODS: In this controlled double-blind study, 38 subjects with chronic periodontitis were randomized into two groups, paired by age to receive ATV (20 mg) or placebo daily for 3 months. Periodontal mechanical treatment was carried out in both groups at baseline. Clinical and radiographic parameters and bone turnover markers were assessed at baseline and at 3 months. RESULTS: Periodontal disease conditions improved in both groups. After comparing the figures of change, significant improvements were observed in cholesterol levels (Δ=-58.5 ± 37.6 versusΔ=5.4 ± 41.2 mg/dl, p<0.0002), low-density lipoprotein levels (Δ=-48.1 ± 31.7 versusΔ=1.9 ± 42.8 mg/dl, p<0.002), dental mobility (Δ=-0.17 ± 0.11 versusΔ=-0.06 ± 0.11%, p<0.04), and the distance from the crestal alveolar bone to the cemento-enamel junction (Δ=-0.75 ± 0.7 versusΔ=0.09 ± 0.4 mm, p<0.0006) in the ATV group. CONCLUSIONS: The results suggest that ATV might have beneficial effects on bone alveolar loss and tooth mobility in subjects with periodontal disease.
