ABSTRACT
Pasteurella species may cause zoonotic infections of humans. Serious systemic infections with these organisms are unusual, but they may occur in individuals with predisposing underlying illnesses. Occurrences of bacteremia due to P. multocida are infrequent, and P. dagmatis bacteremia is even rarer. We report independent occurrences of P. multocida and P. dagmatis septicemia in the same diabetic patient after contact with two pet dogs. We review the history of Pasteurella species and discuss the biochemical and clinical features of its association with zoonosis.
Subject(s)
Bacteremia/microbiology , Diabetes Mellitus/microbiology , Pasteurella Infections/microbiology , Animals , Bacteremia/complications , Bacteremia/transmission , Diabetes Complications , Dogs , Humans , Male , Middle Aged , Pasteurella/classification , Pasteurella/isolation & purification , Pasteurella Infections/complications , Pasteurella Infections/transmission , Pasteurella multocida/classification , Pasteurella multocida/isolation & purification , White People , ZoonosesABSTRACT
Soluble Plasmodium falciparum polypeptides, affinity-purified from culture supernatant fluids using sequential immunoadsorptions employing both monoclonal and polyclonal antibodies, induced protective immunity against experimental falciparum malaria in Peruvian Aotus nancymai monkeys. Susceptible monkeys were vaccinated with polypeptides affinity-purified from supernatant fluids of P. falciparum Indochina I/CDC cultures. Eighteen animals (6 immunized with purified antigens plus adjuvants, 6 injected with only the adjuvant preparation, and 6 untreated) were challenged with whole blood containing monkey-adapted virulent organisms of the Indochina I/CDC strain. Selected hematologic, serologic and parasitologic profiles served as potential indicators of protection. This immunogen, when fortified with an aluminum hydroxide/Quil-A saponin adjuvant combination, elicited good antibody responses to major P. falciparum antigens. Protection in vaccinated animals was evidenced by a significantly limited reduction in hematocrit and hemoglobin levels and a relatively moderate course of infection after homologous needle-challenge with Aotus monkey-adapted P. falciparum parasites.
Subject(s)
Antigens, Protozoan/immunology , Malaria/prevention & control , Plasmodium falciparum/immunology , Protozoan Vaccines , Animals , Antibodies, Protozoan/biosynthesis , Antigens, Protozoan/chemistry , Antigens, Protozoan/isolation & purification , Aotus trivirgatus , Blotting, Western , Chromatography, Affinity , Electrophoresis, Polyacrylamide Gel , Female , Hematocrit , Hemoglobins/analysis , Male , Molecular Weight , Vaccination/adverse effectsABSTRACT
Human erythrocytic culture-adapted parasites of the Geneve/SGE-1 strain of Plasmodium falciparum were successfully adapted to grow in an in vitro culture system containing squirrel monkey erythrocytes and serum. These monkey culture-adapted organisms were then used to produce a patent infection in a splenectomized squirrel monkey. Fresh infected blood from this animal was introduced into another splenectomized monkey and was subsequently serially passed through seven intact squirrel monkeys. High level parasitemias (greater than 10%) were obtained in the animals from the last two passes following inoculation of moderate numbers of parasites. It is anticipated that this squirrel monkey-adapted Geneve/SGE-1 strain of P. falciparum will continue to produce high level parasitemias in intact Bolivian Saimiri, and consequently will be suitable for challenge of these monkeys.
