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1.
Environ Sci Pollut Res Int ; 29(12): 18189-18201, 2022 Mar.
Article in English | MEDLINE | ID: mdl-34687415

ABSTRACT

Novel and sustainable chitosan (CS)/activated charcoal (AC) composites were prepared by cross-linking with epichlorohydrin (ECH) to form a porous structure. Different titanium dioxide nanoparticle (TiO2 NPs) concentrations (0, 0.2, 0.4, and 0.8% w/w) were added to enhance the photocatalytic, antibacterial, larvicidal, and pupicidal activities' efficiency toward Rose Bengal (RB) dye and the Culex pipiens. The composites were characterized by FT-IR, XRD, XPS, BET and SEM. The SEM images revealed the porous structure of CS/AC and TiO2 nanoparticles were uniformly distributed in the CS/AC matrix. The degradation of RB dye was used to test the photocatalytic behavior of the composites. Supporting TiO2 on a CS/AC matrix resulted in a significant increase in photocatalytic performance. The antibacterial activities supported by CS/AC/TiO2 NPs were evaluated by bacterial growth inhibition against B. subtilis, S. aureus, E. coli, and P. aeruginosa. The results showed that CS/AC/TiO2 NPs composite has an inhibitory effect and therefore considered antibacterial agents. CS/AC/0.4%TiO2 NPs showed maximum efficacy against larvicidal activity and pupicidal of mosquito vector which recorded 99.00 ± 1.14, 95.00 ± 1.43, and 92.20 ± 2.64 for the first, second, and third larval instars and 66.00 ± 2.39 for pupal mortality, while the repellent activity reported high protection at 82.95 ± 2.99 with 3.24 mg/cm2 dose compared to control DEET.


Subject(s)
Chitosan , Nanoparticles , Animals , Catalysis , Charcoal/pharmacology , Chitosan/chemistry , Escherichia coli , Spectroscopy, Fourier Transform Infrared , Staphylococcus aureus , Titanium/chemistry , Titanium/pharmacology
2.
Anal Chem ; 92(22): 14999-15006, 2020 11 17.
Article in English | MEDLINE | ID: mdl-33140638

ABSTRACT

The pH of a system is a critical descriptor of its chemistry-impacting reaction rates, solubility, chemical speciation, and homeostasis. As a result, pH is one of the most commonly measured parameters in food safety, clinical, and environmental laboratories. Glass pH probes are the gold standard for pH measurements but suffer drawbacks including frequent recalibration, wet storage of the glass membrane, difficulty in miniaturization, and interferences from alkali metals. In this work, we describe a voltammetric pH sensor that uses a three-dimensional (3D)-printed graphene/poly(lactic acid) filament electrode that is pretreated to introduce quinone functional groups to the graphene surface. After thoroughly characterizing the pretreatment parameters using outer-sphere and inner-sphere redox couples, we measured pH by reducing the surface-bound quinones, which undergo a pH-dependent 2e-/2H+ reduction. The position of the redox peak was found to shift -60 ± 2 mV pH-1 at 25 °C, which is in excellent agreement with the theoretical value predicted by the Nernst Equation (-59.2 mV pH-1). Importantly, the sensors did not require the removal of dissolved oxygen prior to successful pH measurements. We investigated the impact of common interfering species (Pb2+ and Cu2+) and found that there was no impact on the measured pH. We subsequently challenged the sensors to measure the pH of unadulterated complex samples, including cola, vinegar, an antacid tablet slurry, serum, and urine, and obtained excellent agreement compared to a glass pH electrode. In addition to the positive analytical characteristics, the sensors are extremely cheap and easy to fabricate, making them highly accessible to a wide range of researchers. These results pave the way for customizable pH sensors that can be fabricated in (nearly) any geometry for targeted applications using 3D printing.


Subject(s)
Blood Chemical Analysis/instrumentation , Food Analysis/instrumentation , Graphite/chemistry , Polyesters/chemistry , Printing, Three-Dimensional , Urinalysis/instrumentation , Benzoquinones/chemistry , Electrodes , Hydrogen-Ion Concentration , Limit of Detection , Oxidation-Reduction , Surface Properties
3.
J Periodontal Res ; 41(1): 1-9, 2006 Feb.
Article in English | MEDLINE | ID: mdl-16409249

ABSTRACT

OBJECTIVE: High levels of serum anti-Actinobacillus actinomycetemcomitans immunoglobulin G (IgG) correlate with reduced extent and severity of periodontal disease and the present study was undertaken to begin testing the hypothesis that proinflammatory cytokines are important in the induction of optimal anti-A. actinomycetemcomitans IgG responses. BACKGROUND: Studies with pokeweed mitogen indicate that interleukin-1alpha (IL-1alpha) and IL-1beta are necessary for optimal IgG1 and IgG2 production and that prostaglandin E(2) (PGE(2)) and interferon-gamma (IFN-gamma) selectively promote IgG2, which is a major component of the anti-A. actinomycetemcomitans response in vivo. The pokeweed mitogen results suggest that these proinflammatory cytokines would also be necessary for optimal production of IgG specific for A. actinomycetemcomitans. METHODS: Peripheral blood mononuclear cells from A. actinomycetemcomitans-seropositive subjects with localized aggressive periodontitis were stimulated with A. actinomycetemcomitans in immune complexes capable of binding follicular dendritic cells that participate in the induction of recall responses in vivo. Cultures were manipulated with anti-IL-1alpha, anti-IL-1beta, anti-IFN-gamma, anti-IL-12, anti-CD21, indomethacin, and PGE(2). Actinobacillus actinomycetemcomitans specific IgG production was monitored by enzyme-linked immunosorbent assay (ELISA). RESULTS: Addition of follicular dendritic cells to peripheral blood mononuclear cells cultures resulted in follicular dendritic cell-lymphocyte clusters and increased anti-A. actinomycetemcomitans IgG responses (3-40-fold increases) compared with controls lacking follicular dendritic cells. Anti-IL-1alpha, anti-IL-1beta, anti-IFN-gamma, anti-IL-12, anti-CD21 and indomethacin suppressed anti-A. actinomycetemcomitans IgG production by half or more. PGE(2) restored IgG responses suppressed by indomethacin. CONCLUSIONS: The cytokines IL-1alpha, IL-1beta, IFN-gamma, IL-12, and PGE(2) were all necessary for optimal production of human anti-A. actinomycetemcomitans and the need for proinflammatory cytokines including the T helper 1 (Th1) cytokines is consistent with a response with a significant IgG2 component.


Subject(s)
Aggregatibacter actinomycetemcomitans/immunology , Cytokines/immunology , Immunoglobulin G/immunology , Inflammation Mediators/immunology , Actinobacillus Infections/blood , Animals , Anti-Inflammatory Agents, Non-Steroidal/pharmacology , Antigen-Antibody Complex/immunology , Dendritic Cells, Follicular/immunology , Dinoprostone/immunology , Humans , Indomethacin/pharmacology , Interferon-gamma/immunology , Interleukin-1/immunology , Interleukin-12/immunology , Leukocytes, Mononuclear/immunology , Mice , Mice, Inbred BALB C , Periodontitis/blood , Periodontitis/microbiology , Receptors, Complement 3d/immunology , Th1 Cells/immunology
4.
J Periodontol ; 72(10): 1332-9, 2001 Oct.
Article in English | MEDLINE | ID: mdl-11699474

ABSTRACT

BACKGROUND: Serum concentrations of immunoglobulin G2 (IgG2) are elevated in localized aggressive periodontitis (LAgP) patients, and secretory products of monocytes from LAgP patients enhance IgG2 responses of lymphocytes from healthy subjects. Furthermore, genes regulating production of interleukin (IL)-1 influence the risk for both aggressive periodontitis (AgP) and chronic periodontitis. These observations, and the fact that IgG2 dominates responses to carbohydrates from Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis, prompted the hypothesis that IL-1 alpha, IL-1 beta, and IL-RA may help regulate human IgG2 responses. METHODS: Human peripheral blood leukocytes (PBL) were stimulated in culture with pokeweed mitogen (PWM); the levels of available IL-1 gene products were manipulated; and the effect on IgG2 production was monitored. Manipulations of IL-1 were accomplished by adding specific neutralizing monoclonal antibodies or recombinant IL-1RA, IL-1 alpha, or IL-1 beta. RESULTS: Blocking the IL-1 receptor with IL-1RA or neutralizing IL-1 alpha or IL-1 beta with specific antibody dramatically suppressed IgG2 production (50% to 70%). Additionally IL-1 alpha did not compensate for neutralized IL-1 beta, and additional IL-1 beta did not compensate for neutralized IL-1 alpha, suggesting the 2 monokines have separate roles in promoting IgG2. Furthermore, combinations of anti-IL-1 alpha and anti-IL-1 beta were more inhibitory than either antibody alone, and IL-1 alpha and IL-1 beta in combination appeared to work additively in promoting IgG2. Moreover, PBL cultures from a group of LAgP patients with high IgG2 levels had elevated levels of IL-1 beta. CONCLUSION: IL-1 alpha and IL-1 beta appear to have critical and non-redundant roles in the generation and regulation of potent IgG2 responses, which appear to be important in human responses to carbohydrate-bearing bacteria.


Subject(s)
Immunoglobulin G/immunology , Interleukin-1/immunology , Periodontitis/immunology , Adult , Aggregatibacter actinomycetemcomitans/immunology , Analysis of Variance , Antibodies, Monoclonal , Carbohydrates/immunology , Cells, Cultured , Chronic Disease , Gene Expression Regulation , Humans , Immunoglobulin G/blood , Interleukin-1/blood , Interleukin-1/genetics , Leukocytes, Mononuclear/immunology , Lymphocytes/immunology , Monocytes/metabolism , Monokines/immunology , Periodontitis/blood , Periodontitis/classification , Pokeweed Mitogens , Porphyromonas gingivalis/immunology , Receptors, Interleukin-1/immunology , Recombinant Proteins , Statistics as Topic
5.
Trends Immunol ; 22(7): 361-7, 2001 Jul.
Article in English | MEDLINE | ID: mdl-11429319

ABSTRACT

Follicular dendritic cells (FDCs) are potent accessory cells for B cells, but the molecular basis of their activity is not understood. Several important molecules involved in FDC-B-cell interactions are indicated by blocking the ligands and receptors on FDCs and/or B cells. The engagement of CD21 in the B-cell coreceptor complex by complement-derived CD21 ligand on FDCs delivers a crucial signal that dramatically augments the stimulation delivered by the binding of antigen to the B-cell receptor (BCR). The engagement of Fc gamma receptor IIB (FcgammaRIIB) by the Ig crystallizable fragment (Fc) in antigen-antibody complexes held on FDCs decreases the activation of immunoreceptor tyrosine-based inhibition motifs (ITIMs), mediated by the crosslinking of BCR and FcgammaRIIB. Thus, FDCs minimize a negative B-cell signal. In short, these ligand-receptor interactions help to signal to B cells and meet a requirement for B-cell stimulation that goes beyond the necessity of T-cell help.


Subject(s)
B-Lymphocytes/immunology , Dendritic Cells, Follicular/immunology , T-Lymphocytes, Helper-Inducer/immunology , Animals , Antigens, CD/immunology , Histocompatibility Antigens Class II/immunology , Humans , Ligands , Receptors, Complement 3d/immunology , Receptors, IgG/immunology
6.
J Immunol ; 166(8): 4863-9, 2001 Apr 15.
Article in English | MEDLINE | ID: mdl-11290762

ABSTRACT

Serum IgE is suppressed in CD23-transgenic (Tg) mice where B cells and some T cells express high levels of CD23, suggesting that CD23 on B and T cells may cause this suppression. However, when Tg B lymphocytes were compared with controls in B cell proliferation and IgE synthesis assays, the two were indistinguishable. Similarly, studies of lymphokine production suggested that T cell function in the Tg animals was normal. However, adoptive transfer studies indicated that suppression was seen when normal lymphocytes were used to reconstitute Tg mice, whereas reconstitution of controls with Tg lymphocytes resulted in normal IgE responses, suggesting that critical CD23-bearing cells are irradiation-resistant, nonlymphoid cells. Follicular dendritic cells (FDC) are irradiation resistant, express surface CD23, and deliver iccosomal Ag to B cells, prompting us to reason that Tg FDC may be a critical cell. High levels of transgene expression were observed in germinal centers rich in FDC and B cells, and IgE production was inhibited when Tg FDCs were cultured with normal B cells. In short, suppressed IgE production in CD23-Tg mice appears to be associated with a population of radioresistant nonlymphoid cells. FDCs that interface with B cells in the germinal center are a candidate for explaining this CD23-mediated IgE suppression.


Subject(s)
Dendritic Cells, Follicular/immunology , Dendritic Cells, Follicular/metabolism , Down-Regulation/genetics , Down-Regulation/immunology , Immunoglobulin E/biosynthesis , Mice, Transgenic/immunology , Receptors, IgE/biosynthesis , Receptors, IgE/genetics , Adoptive Transfer , Animals , B-Lymphocytes/immunology , B-Lymphocytes/metabolism , Cells, Cultured , Cytokines/biosynthesis , Dendritic Cells, Follicular/radiation effects , Down-Regulation/radiation effects , Gene Expression Regulation/immunology , Immunoglobulin E/blood , Lymphocyte Activation/genetics , Lymphocyte Transfusion , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Radiation Tolerance/immunology , Receptors, IgE/immunology , Spleen/cytology , Spleen/radiation effects , Spleen/transplantation , Th1 Cells/immunology , Th1 Cells/metabolism , Th2 Cells/immunology , Th2 Cells/metabolism , Transgenes/immunology
7.
Eur J Immunol ; 31(1): 176-85, 2001 Jan.
Article in English | MEDLINE | ID: mdl-11169451

ABSTRACT

Productive follicular dendritic cell (FDC)-B cell interactions appear to involve critical ligand-receptor interactions. Immune complexes (IC) on FDC activate complement and provide FDC with a complement-derived CD21 ligand (CD21L), which bind CD21, while antigen in the IC binds on the B cell-BCR. Further, FDC-FcgammaRIIB binds Fc regions of antibodies in IC and reduces coligation of BCR and FcgammaRIIB minimizing an inhibitor of B cell activation. Given that Fc receptors and complement receptors bind immunoglobulins and complement fragments of other species, we reasoned that FDC accessory activity should cross MHC and species barriers. This prediction was tested using memory lymphocytes from OVA-immune mice and TT-immune humans in combination with FDC from murine lymph nodes and human tonsils. Human and murine FDC converted IC into potent immunogens (specific antibody increased from background to thousands of ng / ml). MHC barriers did not restrict this activity and human FDC worked with murine lymphocytes and murine FDC worked with human lymphocytes. Furthermore, stimulation via MHC-dependent allogeneic or zenogeneic mechanisms did not promote antibody production by FDC. Polyclonal responses stimulated by lipopolysaccharide and pokeweed mitogen were also promoted (10 - 100-fold) and anti-CD21 blocked FDC activity. These results substantiate the hypothesis that FDC are necessary for strong recall responses and that FDC-CD21L is critical.


Subject(s)
Dendritic Cells, Follicular/physiology , Major Histocompatibility Complex , Animals , Cells, Cultured , Child , Child, Preschool , Female , Humans , Mice , Mice, Inbred BALB C , Mice, Inbred C3H , Mice, Inbred C57BL , Ovalbumin/immunology , Receptors, Antigen, B-Cell/physiology , Receptors, Complement 3d/physiology , Species Specificity
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