[MОRPHOLOGICAL HEPATIC CHANGES IN RATS WHILE CCL(4) ­Ð†NDUCED CIRRHOSIS AND HEPATIC ENCEPHALOPATHY ON BACKGROUND OF TREATMENT].

Impact of choline alphoscerate on morphological hepatic indices in rats with hepatic cirrhosis and hepatic encephalopathy, caused by injection of 15% solution of CCl(4) during 4 weeks, in comparison to standard scheme of treatment, was studied. Reduction of the hepatic histological changes severity, of the injured hepatocytes volume, of stromal­parenchymal ratio and enhancement of nucleo­cytoplasmic ratio in rats with hepatic cirrhosis under the choline alphoscerate impact were noted. The fibrosis and histological activity index in accordance to Knodell's scale witnessed a reduction of severity of hepatic affection in rats, which were treated, using choline alphoscerate.

ESTIMATION OF THE CYTOKINS LEVEL IN EXPERIMENTAL HEPATIC ENCEPHALOPATHY IN RATS.

Connection of the cytokins profile with experimental hepatic encephalopathy (HE) in rats was studied. Investigation was conducted on 20 laboratory rats, in which HE was simulated, using СCl4 injection. The іnterleukins (ІL) level, including, ІL­1ß, ІL­4, ІL­10 and interferon­Î³(IFN­Î³), was determined using immunoassay method with the help of polyclonal antibodies. Enhancement of the proinflammatory ІL­1ß and ІНФ­Î³ content in the rats blood serum while induced HE by 57.9 and 39.5% accordingly (p <0.05), comparing with such in a control and the compensation enhancement of the anti'inflammatory IL­4 and IL­10 level by 34.6 and 75.9% (p < 0.05) was established. The results obtained confirms the cytokins role in pathogenesis of HE, determination of their level constitutes significant criterion for the posttransplantation complications prog' nostication. Inflammation and profile of cytokins constitute the main target in therapy of HE in patients, suffering liver cirrhosis