ABSTRACT
Terminal restriction fragment length polymorphism and quantitative PCR showed that the cecal microbiota of chicks up to the age of 21 days was dominated by representatives of the orders Enterobacteriales, Clostridiales, and Lactobacillales. Salmonella enterica serovar Enteritidis infection caused the greatest changes in the gut microbiota when 1-day-old chicks were infected, compared with the infection of 4- and 16-day-old chicks.
Subject(s)
Cecum/microbiology , Metagenome , Salmonella Infections, Animal/microbiology , Salmonella enteritidis/growth & development , Animals , Animals, Newborn , Chickens , Polymerase Chain ReactionABSTRACT
In a collection of 66 Salmonella enterica serovar Typhimurium strains isolated between 1984 and 2002 in the Czech Republic, genes coding for antibiotic resistance were determined by using specific PCRs. We found that the pentadrug-resistant ACSSuT clone first appeared in the Czech Republic in 1990. A new variant of the aadA gene designated aadA21 is described, the 5' end of which was identical to aadA2 and the 3' end of which was identical to aadA1.
Subject(s)
Drug Resistance, Multiple, Bacterial/genetics , Salmonella Infections/microbiology , Salmonella typhimurium/drug effects , Animals , Animals, Domestic , Czech Republic , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , Humans , Microbial Sensitivity Tests , Polymerase Chain Reaction , Retrospective Studies , Salmonella typhimurium/genetics , Salmonella typhimurium/isolation & purificationABSTRACT
Computer-assisted restriction endonuclease analysis of plasmid DNA in field strains of Salmonella enterica serovar Enteritidis (S. enteritidis) is described. The procedure consists of plasmid DNA purification, its digestion with restriction endonuclease TaqI, electrophoresis, charge-coupled device camera scanning of the gels, and an analysis of the restriction patterns with the software Gel Manager. The system allowed us to analyse, in detail, results of plasmid profiling in more than 600 field strains of S. enteritidis. In addition to plasmid-free and virulence plasmid only containing strains, 15 additional plasmid types were detected. All the images and detailed protocols are available at the Web site http://www.clark.cz/vri/salmon.htm.
