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1.
Methods Mol Biol ; 693: 321-43, 2011.
Article in English | MEDLINE | ID: mdl-21080290

ABSTRACT

Recombinant adenoviruses are attractive vectors for short-term expression in mouse liver and primary cell lines. Various versatile vector systems have been developed which can be used for the reliable production of recombinant adenoviruses. This protocol describes the entire process for the production of recombinant adenoviruses using the AdEasy system. This protocol will give a practical step-by-step description from the cloning of the gene of interest until the in vivo administration in mice. The entire process will take about 8 weeks to complete.


Subject(s)
Adenoviridae/genetics , Gene Transfer Techniques , Genetic Vectors/genetics , Animals , Cells, Cultured , Liver/metabolism , Mice , Models, Genetic , Recombination, Genetic/genetics , Transduction, Genetic
2.
Restor Neurol Neurosci ; 25(5-6): 585-99, 2007.
Article in English | MEDLINE | ID: mdl-18418947

ABSTRACT

PURPOSE: Spinal root avulsions result in paralysis of the upper and/or lower extremities. Implanting a peripheral nerve bridge or reinsertion of the avulsed roots in the spinal cord are surgical strategies that lead to some degree of functional recovery. In the current study lentiviral (LV) vector-mediated gene transfer of a green fluorescent protein (GFP) reporter gene was used to study the feasibility of gene therapy in the reimplanted root to further promote regeneration of motor axons. METHODS: A total of 68 female Wistar rats underwent unilateral root avulsion of the L4, L5 and L6 ventral lumbar roots. From 23 rats intercostal nerves were dissected before ventral root avulsion surgery, injected with a lentiviral vector encoding GFP (LV-GFP) and inserted between the spinal cord and avulsed rootlet. In the remaining 45 rats, the avulsed ventral root was injected with either LV-GFP or a lentiviral vector encoding a fusion between a GlyAla repeat and GFP (LV-GArGFP), and reinserted into the spinal cord. Expression of GFP was evaluated at 1,2, 4 and 10 weeks, and one group at 4 months. RESULTS: LV-GFP transduction of either nerve implants or reimplanted ventral roots revealed high GFP expression during the first 2 post-lesion weeks, but virtually no expression at 4 weeks. Since this reduction coincided with the appearance of mononuclear cells at the repair site, an immune response against GFP may have occurred. In a subsequent experiment reimplanted ventral roots were transduced with a vector encoding GFP fused with the GlyAla repeat of Epstein-Barr virus Nuclear Antigen 1 known to prevent generation of antigenic peptides from transgene products. Expression of this "stealth" gene persisted for at least 4 months in the reimplanted root. CONCLUSION: Thus persistent transgene expression can be achieved with non-immunogenic transgene products in reimplanted ventral roots. This demonstrates the feasibility of combining neurosurgical repair with LV vector-mediated gene therapy. The current approach will be used in future experiments with LV vectors encoding neurotrophic factors to enhance the regeneration of spinal motor neurons after traumatic avulsion of spinal nerve roots.


Subject(s)
Gene Expression/physiology , Genetic Vectors/physiology , Green Fluorescent Proteins/metabolism , Lentivirus/physiology , Nerve Regeneration/physiology , Spinal Cord Injuries/pathology , Spinal Nerve Roots/metabolism , Animals , Disease Models, Animal , Female , Gene Transfer Techniques , Rats , Rats, Wistar , Spinal Cord Injuries/therapy , Spinal Nerve Roots/injuries , Time Factors
3.
BMC Biotechnol ; 6: 11, 2006 Feb 28.
Article in English | MEDLINE | ID: mdl-16507095

ABSTRACT

BACKGROUND: Adenovirus (Ad) mediated gene transfer is a well-established tool to transiently express constructs in livers of mice in vivo. In the present study, we determined the specificity and efficiency of Ad vectors expressing short hairpin (sh) RNA constructs to knock-down the estrogen receptor alpha (ERalpha). RESULTS: Two different shRNA constructs derived from the murine ERalpha coding sequence were designed (shERalpha). In vitro, transfection of three mouse cell lines with pSUPER-shERalpha constructs resulted in up to 80% reduction of endogenous ERalpha activity. A single mismatch in the target sequence eliminated the reduction of ERalpha activity, demonstrating the specificity of shERalpha. The subsequently generated Ad.shERalpha vectors were equally effective in vitro. In vivo, intravenous administration of Ad.shERalpha resulted in 70% reduced hepatic mouse ERalpha mRNA levels. Co-injection of Ad.shERalpha with an Ad vector containing a luciferase (luc) gene driven by an estrogen responsive element (ERE) containing promoter resulted in a significant (90% on day five) down-regulation of hepatic luciferase activity, as determined by non-invasive optical imaging. Down-regulation was sustained up to day seven post-injection. CONCLUSION: Ad mediated transfer of shERalpha expression constructs results in efficient and specific knockdown of endogenous ERalpha transcription both in vitro and in vivo.


Subject(s)
Adenoviridae/genetics , Estrogen Receptor alpha/deficiency , Estrogen Receptor alpha/genetics , Liver/metabolism , RNA, Viral/genetics , Transfection/methods , Animals , Cell Line , Gene Silencing , Genetic Vectors/genetics , Mice , Mice, Knockout , RNA, Viral/administration & dosage , Recombinant Proteins/metabolism
4.
J Gen Virol ; 87(Pt 3): 553-562, 2006 Mar.
Article in English | MEDLINE | ID: mdl-16476976

ABSTRACT

The fowl adenovirus 1 (FAdV-1) isolates PHELPS and OTE are highly similar, but have striking differences in the repeat region of the inverted terminal repeat (ITR). Whilst the repeat region in OTE conforms to the conventional human adenovirus repeat region (5'-CATCATC), that of PHELPS contains guanidine residues at positions 1, 4 and 7 (5'-GATGATG). This implies that the FAdV-1 isolates PHELPS and OTE have either distinct template specificity at replication initiation or, alternatively, a relaxed specificity for replication initiation. In this study, the distinct sequence variation at the origin of DNA replication in the ITRs of the FAdV-1 PHELPS and OTE isolates was confirmed. Sequence analyses of the pTP and Pol genes of both PHELPS and OTE did not reveal differences that could explain the distinct template specificity. Replication assays demonstrated that linear DNA fragments flanked by either 5'-CATCATC or 5'-GATGATG termini replicated in cells upon infection with FAdV-1 OTE and FAdV-1 PHELPS. This was evident from the appearance of DpnI-resistant fragments in a minireplicon assay. From these data, it is concluded that FAdV-1 has relaxed, rather than changed, its template specificity at replication initiation.


Subject(s)
Fowl adenovirus A/genetics , Templates, Genetic , Terminal Repeat Sequences/genetics , Amino Acid Sequence , Base Sequence , DNA, Viral/biosynthesis , Gene Products, pol/genetics , Molecular Sequence Data , Phosphoproteins/genetics , Protein Precursors/genetics , Sequence Alignment , Species Specificity , Viral Proteins/genetics
5.
J Virol ; 78(7): 3470-9, 2004 Apr.
Article in English | MEDLINE | ID: mdl-15016870

ABSTRACT

The efficiency and specificity of gene transfer with human adenovirus (hAd)-derived gene transfer vectors would be improved if the native viral tropism could be modified. Here, we demonstrate that the minor capsid protein IX (pIX), which is present in 240 copies in the Ad capsid, can be exploited as an anchor for heterologous polypeptides. Protein IX-deleted hAd5 vectors were propagated in hAd5 helper cells expressing pIX variants, with heterologous carboxyl-terminal extensions of up to 113 amino acids in length. The extensions evaluated consist of alpha-helical spacers up to 75 A in length and to which peptide ligands were fused. The pIX variants were efficiently incorporated into the capsids of Ad particles. On intact particles, the MYC-tagged-pIX molecules were readily accessible to anti-MYC antibodies, as demonstrated by electron microscopic analyses of immunogold-labeled virus particles. The labeling efficiency improved with increasing spacer length, suggesting that the spacers lift and expose the ligand at the capsid surface. Furthermore, we found that the addition of an integrin-binding RGD motif to the pIX markedly stimulated the transduction of coxsackievirus group B and hAd receptor-deficient endothelioma cells, demonstrating the utility of pIX modification in gene transfer. Our data demonstrate that the minor capsid protein IX can be used as an anchor for the addition of polypeptide ligands to Ad particles.


Subject(s)
Adenoviridae/physiology , Capsid Proteins/chemistry , Capsid Proteins/metabolism , Genetic Engineering , Recombinant Fusion Proteins/chemistry , Recombinant Fusion Proteins/metabolism , Adenoviridae/genetics , Animals , Blotting, Western , Capsid Proteins/genetics , Cell Line , Cell Line, Tumor , Humans , Ligands , Mice , Microscopy, Immunoelectron , Mutation/genetics , Protein Binding , Protein Transport , Receptors, Virus/metabolism , Recombinant Fusion Proteins/genetics , Substrate Specificity
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