1.
Biotechnology
; 24: 17-27, 1992.
Article
in English
| MEDLINE
| ID: mdl-1422010
2.
Biotechnology
; 24: 476-80, 1992.
Article
in English
| MEDLINE
| ID: mdl-1422056
3.
Methods Enzymol
; 155: 335-50, 1987.
Article
in English
| MEDLINE
| ID: mdl-3431465
4.
Cold Spring Harb Symp Quant Biol
; 51 Pt 1: 263-73, 1986.
Article
in English
| MEDLINE
| ID: mdl-3472723
5.
Science
; 230(4732): 1350-4, 1985 Dec 20.
Article
in English
| MEDLINE
| ID: mdl-2999980
ABSTRACT
Two new methods were used to establish a rapid and highly sensitive prenatal diagnostic test for sickle cell anemia. The first involves the primer-mediated enzymatic amplification of specific beta-globin target sequences in genomic DNA, resulting in the exponential increase (220,000 times) of target DNA copies. In the second technique, the presence of the beta A and beta S alleles is determined by restriction endonuclease digestion of an end-labeled oligonucleotide probe hybridized in solution to the amplified beta-globin sequences. The beta-globin genotype can be determined in less than 1 day on samples containing significantly less than 1 microgram of genomic DNA.