ABSTRACT
A multifunctional nanoseaurchin probe in which mesoporous silica nanobeads with iron oxide nanoparticles embedded and multi-gold nanorods crystal-seeded are fabricated and labeled with umbilical cord mesenchymal stem cells through endocytosis. This nanoplatform enables efficient magnetic remote-controlled guiding for stem cell homing, and provides dual modalities of photoacoustic imaging and magnetic resonance imaging for in situ tracking and long-term monitoring to achieve therapeutic efficacy.
Subject(s)
Gold/chemistry , Magnetite Nanoparticles/chemistry , Mesenchymal Stem Cell Transplantation , Nanotubes/chemistry , Stroke/therapy , Animals , Antigens, CD/metabolism , Brain/diagnostic imaging , Brain/pathology , Cell Differentiation/drug effects , Cell Proliferation/drug effects , Cells, Cultured , Contrast Media/chemistry , Ferrosoferric Oxide/chemistry , Flow Cytometry , Humans , Male , Mesenchymal Stem Cells/cytology , Mesenchymal Stem Cells/metabolism , Mice , Mice, Inbred C57BL , Nanotubes/toxicity , Nanotubes/ultrastructure , Radiography , Wharton Jelly/cytologyABSTRACT
Nanomedicine can provide a multi-functional platform for image-guided diagnosis and treatment of cancer. Although gold nanorods (GNRs) have been developed for photoacoustic (PA) imaging and near infra-red (NIR) photothermal applications, their efficiency has remained limited by low thermal stability. Here we present the synthesis, characterization, and functional evaluation of non-cytotoxic magnetic polymer-modified gold nanorods (MPGNRs), designed to act as dual magnetic resonance imaging (MRI) and PA imaging contrast agents. In addition, their high magnetization allowed MPGNRs to be actively localized and concentrated by targeting with an external magnet. Finally, MPGNRs significantly enhanced the NIR-laser-induced photothermal effect due to their increased thermal stability. MPGNRs thus provide a promising new theranostic platform for cancer diagnosis and treatment by combining dual MR/PA imaging with highly effective targeted photothermal therapy.
Subject(s)
Acrylamides/chemistry , Gold/chemistry , Hyperthermia, Induced/methods , Magnetic Resonance Spectroscopy , Nanotubes/chemistry , Photoacoustic Techniques/methods , Phototherapy/methods , Polymers/chemistry , Acrylic Resins , Animals , Cell Line, Tumor , Magnetics , Metal Nanoparticles/ultrastructure , Mice , Microscopy, Fluorescence , Nanotubes/ultrastructure , Phantoms, Imaging , Rats , Spectrophotometry, Ultraviolet , TemperatureABSTRACT
The novel nano-seaurchin structure is characteristic of high-density and well-dispersed gold nanorods in one mesoporous silica nanobead. This nanoplatform provided increased photothermal stability, stable photoacoustic signal and highly efficient hyperthermia effect both in vitro and in vivo, indicating a powerful theranostic modality.
