ABSTRACT
BACKGROUND: This study investigated the influence of curcumin on HOX transcript antisense RNA (HOTAIR)- mediated migration of cultured renal cell carcinoma (RCC) cells. MATERIALS AND METHODS: Five RCC cell lines (769-P, 769-P-vector, 769-P-HOTAIR, 786-0, and Kert-3 ) were maintained in vitro. The expression of HOTAIR mRNA was determined by quantitative real-time PCR and cell migration was measured by transwell migration assay. The effects of different concentrations of curcumin (0 to 80 µmol/L) on cell proliferation was determined by the CCK-8 assay and influence of non-toxic levels (0 to 10 µM) on the migration of RCC cells was also determined. RESULTS: Comparison of the 5 cell lines indicated a correlation between HOTAIR mRNA expression and cell migration. In particular, the migration of 769-P-HOTAIR cells was significantly higher than that of 769-P-vector cells. Curcumin at 2.5-10 µM had no evident toxicity against RCC cells, but inhibited cell migration in a concentration-dependent manner. CONCLUSIONS: HOTAIR expression is correlated with the migration of RCC cells, and HOTAIR may be involved in the curcumin-induced inhibition of RCC metastasis.
