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1.
Mol Biol Rep ; 47(12): 9259-9269, 2020 Dec.
Article in English | MEDLINE | ID: mdl-33159233

ABSTRACT

Previous studies have demonstrated that transcription factor Etv5 plays an important role in the segregation between epiblast and primitive endoderm at the second fate decision of early embryo. However, it remains elusive whether Etv5 functions in the segregation between inner cell mass and trophectoderm at the first cell fate decision. In this study, we firstly generated Etv5 knockout mouse embryonic stem cells (mESCs) by CRISPR/Cas9, then converted them into extended potential stem cells (EPSCs) by culturing the cells in small molecule cocktail medium LCDM (LIF, CHIR99021, (S)-(+)-dimethindene maleate, minocycline hydrochloride), and finally investigated their differentiation efficiency of trophoblast stem cells (TSCs). The results showed that Etv5 knockout significantly decreased the efficiency of TSCs (CDX2+) differentiated from EPSCs. In addition, Etv5 knockout resulted in higher incidence of the differentiated cells with tetraploid and octoploid than that from wild type. Mechanistically, Etv5 was activated by extracellular-signal-regulated kinase (ERK) signaling pathway; in turn, Etv5 had a positive feedback on the expression of fibroblast growth factor receptor 2 (FGFR2) which lies upstream of ERK. Etv5 knockout decreased the expression of FGFR2, whose binding with fibroblast growth factor 4 was essentially needed for TSCs differentiation. Collectively, the findings in this study suggest that Etv5 is required to safeguard the TSCs differentiation by regulating FGFR2 and provide new clues to understand the specification of trophectoderm in vivo.


Subject(s)
Cell Differentiation/genetics , DNA-Binding Proteins/metabolism , MAP Kinase Signaling System/genetics , Mouse Embryonic Stem Cells/metabolism , Receptor, Fibroblast Growth Factor, Type 2/metabolism , Transcription Factors/metabolism , Trophoblasts/cytology , Trophoblasts/metabolism , Animals , Benzamides/pharmacology , CRISPR-Cas Systems , Cells, Cultured , Culture Media , DNA-Binding Proteins/genetics , Dimethindene/pharmacology , Diphenylamine/analogs & derivatives , Diphenylamine/pharmacology , Embryonic Development/genetics , Gene Knockout Techniques , MAP Kinase Signaling System/drug effects , Mice , Minocycline/pharmacology , Mouse Embryonic Stem Cells/drug effects , Transcription Factors/genetics , Transfection
2.
Am J Vet Res ; 81(6): 521-526, 2020 Jun.
Article in English | MEDLINE | ID: mdl-32436789

ABSTRACT

OBJECTIVE: To establish a method for evaluation of the efficacy of a classical swine fever virus (CSFV) subunit vaccine in rabbits as determined via humoral immune responses to the virus. ANIMALS: 40 specific pathogen-free rabbits. PROCEDURES: Rabbits were randomly assigned to 4 groups (10 rabbits/group) for SC injection of 0.05, 0.1, and 0.2 mL of a CSFV subunit E2 vaccine (representing 1.15, 2.3, or 4.6 µg of E2 protein/dose, respectively) or saline (0.9% NaCl) solution. Blood samples were collected 21 days after vaccination for measurement of the antibody response against CSFV via ELISA and virus neutralization methods. On the same day, the CSFV Chinese (C) strain was injected into an ear vein. Vaccine efficacy was determined by monitoring of rabbits for pyrexia for 4 days and measurement of viral copies in spleen lysates at the end of the study. Reproducibility of the antibody response was tested with 2 other batches of the vaccine at the minimum immunization dose identified for the initially tested batch. RESULTS: The E2 protein dose of the initially tested vaccine was positively correlated with the antibody response and protection rate in rabbits. The identified minimum immunization dose per rabbit was 0.1 mL, representing an E2 protein content of approximately 2.3 µg, and reproducibility of the antibody response to vaccination with the 2 other batches at this dose was good. CONCLUSIONS AND CLINICAL RELEVANCE: A method was established in rabbits for evaluation of the efficacy of a CSFV subunit vaccine that could help in the optimization of later large-scale vaccine production and quality control processes as well as in the clinical application of the vaccine.


Subject(s)
Classical Swine Fever Virus , Classical Swine Fever , Viral Vaccines , Animals , Antibodies, Viral , Rabbits , Reproducibility of Results , Swine , Vaccines, Subunit , Viral Envelope Proteins
3.
Front Microbiol ; 11: 105, 2020.
Article in English | MEDLINE | ID: mdl-32153518

ABSTRACT

Porcine hemagglutinating encephalomyelitis virus (PHEV) is a single-stranded RNA coronavirus that causes nervous dysfunction in the infected hosts and leads to widespread alterations in the host transcriptome by modulating specific microRNA (miRNA) levels. MiRNAs contribute to RNA virus pathogenesis by promoting antiviral immune response, enhancing viral replication, or altering miRNA-mediated host gene regulation. Thus, exploration of the virus-miRNA interactions occurring in PHEV-infected host may lead to the identification of novel mechanisms combating the virus life cycle or pathogenesis. Here, we discovered that the expression of miR-10a-5p was constitutively up-regulated by PHEV in both the N2a cells in vitro and mice brain in vivo. Treatment with miR-10a-5p mimics allowed miR-10a-5p enrichment and resulted in a significant restriction in PHEV replication, suggesting widespread negative regulation of the RNA virus infection by miR-10a-5p. The outcomes were also evidenced by miR-10a-5p inhibitor over-expression. Luciferase reporter, quantitative real-time PCR (qRT-PCR), and western blotting analysis further showed that Syndecan 1 (SDC1), a cell surface proteoglycan associated with host defense mechanisms, acts as a target gene of miR-10a-5p during PHEV infection. Naturally, siRNA-mediated knockdown of SDC1 leads to a reduction in viral replication, implying that SDC1 expression is likely a favorable condition for viral replication. Together, the findings demonstrated that the abundant miR-10a-5p leads to downstream suppression of SDC1, and it functions as an antiviral mechanism in the PHEV-induced disease, providing a potential strategy for the prevention and treatment of PHEV infection in the future work.

4.
Front Microbiol ; 11: 613437, 2020.
Article in English | MEDLINE | ID: mdl-33613465

ABSTRACT

The H9N2 avian influenza virus is not only an important zoonotic pathogen, it can also easily recombine with other subtypes to generate novel reassortments, such as the H7N9 virus. Although H9N2 live attenuated vaccines can provide good multiple immunities, including humoral, cellular, and mucosal immunity, the risk of reassortment between the vaccine strain and wild-type virus is still a concern. Here, we successfully rescued an H9N2 live attenuated strain [rTX-NS1-128 (mut)] that can interdict reassortment, which was developed by exchanging the mutual packaging signals of HA and truncated NS1 genes and confirmed by RT-PCR and sequencing. The dynamic growth results showed that rTX-NS1-128 (mut) replication ability in chick embryos was not significantly affected by our construction strategy compared to the parent virus rTX strain. Moreover, rTX-NS1-128 (mut) had good genetic stability after 15 generations and possessed low pathogenicity and no contact transmission characteristics in chickens. Furthermore, chickens were intranasally immunized by rTX-NS1-128 (mut) with a single dose, and the results showed that the hemagglutination inhibition (HI) titers peaked at 3 weeks after vaccination and lasted at least until 11 weeks. The cellular immunity (IL-6 and IL-12) and mucosal immunity (IgA and IgG) in the nasal and trachea samples were significantly increased compared to inactivated rTX. Recombinant virus provided a good cross-protection against homologous TX strain (100%) and heterologous F98 strain (80%) challenge. Collectively, these data indicated that rTX-NS1-128(mut) lost the ability for independent reassortment of HA and NS1-128 and will be expected to be used as a potential live attenuated vaccine against H9N2 subtype avian influenza.

5.
Arch Virol ; 165(2): 345-354, 2020 Feb.
Article in English | MEDLINE | ID: mdl-31834525

ABSTRACT

Porcine hemagglutinating encephalomyelitis virus (PHEV) is a typical neurotropic coronavirus that mainly invades the central nervous system (CNS) in piglets and causes vomiting and wasting disease. Emerging evidence suggests that PHEV alters microRNA (miRNA) expression profiles, and miRNA has also been postulated to be involved in its pathogenesis, but the mechanisms underlying this process have not been fully explored. In this study, we found that PHEV infection upregulates miR-142a-3p RNA expression in N2a cells and in the CNS of mice. Downregulation of miR-142a-3p by an miRNA inhibitor led to a significant repression of viral proliferation, implying that it acts as a positive regulator of PHEV proliferation. Using a dual-luciferase reporter assay, miR-142a-3p was found to bind directly bound to the 3' untranslated region (3'UTR) of Rab3a mRNA and downregulate its expression. Knockdown of Rab3a expression by transfection with an miR-142a-3p mimic or Rab3a siRNA significantly increased PHEV replication in N2a cells. Conversely, the use of an miR-142a-3p inhibitor or overexpression of Rab3a resulted in a marked restriction of viral production at both the mRNA and protein level. Our data demonstrate that miR-142a-3p promotes PHEV proliferation by directly targeting Rab3a mRNA, and this provides new insights into the mechanisms of PHEV-related pathogenesis and virus-host interactions.


Subject(s)
Betacoronavirus 1/genetics , Cell Proliferation/genetics , Coronavirus Infections/genetics , MicroRNAs/genetics , Swine/virology , rab3A GTP-Binding Protein/genetics , 3' Untranslated Regions/genetics , Animals , Cell Line , Cell Line, Tumor , Coronavirus Infections/veterinary , Coronavirus Infections/virology , Down-Regulation/genetics , HEK293 Cells , Humans , Mice , RNA, Messenger/genetics , RNA, Small Interfering/genetics , Up-Regulation/genetics
6.
PLoS One ; 14(8): e0219750, 2019.
Article in English | MEDLINE | ID: mdl-31369566

ABSTRACT

BACKGROUND: The cocirculation of duck hepatitis A virus subtypes 1 (DHAV-1) and 3 (DHAV-3) in ducklings has resulted in significant economic losses. Ducklings with DHAV-1 or DHAV-3 infection show similar clinical signs and gross lesions; hence, it is important to identify the viral subtypes in infected ducklings as early as possible for better clinical management. METHODS AND RESULTS: Based on multiple 5' noncoding region (5'-NCR) sequences of DHAV-1 and DHAV-3 strain alignments, universal and type-specific primers were designed and synthesized. With three primers in one-tube reverse transcription-PCR (RT-PCR), reference DHAV-1 and DHAV-3 isolates ranging over 60 years and across many different countries were successfully amplified, indicating that the primer sequences were completely conserved. The sequence results and the sizes of amplicons from reference DHAV-1 and DHAV-3 isolates are completely correlated with their subtypes. Moreover, with this one-tube RT-PCR system, amplicon sizes from liver samples of reference DHAV-1- or DHAV-3-infected birds fit closely with their subtypes, which was determined by virus isolation and neutralization testing. No other duck-origin RNA viruses were detected. The sensitivity of viral RNA detection was 10 pg. With this system, 20% subtype 1, 45% subtype 3, and 9% coinfection of two subtypes were detected in 55 clinical samples. CONCLUSIONS AND SIGNIFICANCE: This novel approach could be used for rapidly typing DHAV-1 or DHAV-3 infection in routine clinical surveillance or epidemiological screening.


Subject(s)
Ducks/virology , Hepatitis Virus, Duck/classification , Hepatitis Virus, Duck/genetics , Hepatitis, Viral, Animal/diagnosis , Picornaviridae Infections/veterinary , Poultry Diseases/epidemiology , Reverse Transcriptase Polymerase Chain Reaction/veterinary , Animals , Genotype , Hepatitis Virus, Duck/isolation & purification , Hepatitis, Viral, Animal/virology , Picornaviridae Infections/diagnosis , Picornaviridae Infections/virology , Poultry Diseases/virology
7.
Vaccine ; 25(46): 7900-8, 2007 Nov 14.
Article in English | MEDLINE | ID: mdl-17923174

ABSTRACT

A new subunit vaccine against infectious bursal disease (IBD) was developed; the antigen used in the vaccine was expressed by a new engineering strain, E. coli BL21/pBV220-VP2. The study on the production and use of the vaccine was performed. The results showed that the recombinant VP2 was water-soluble and demonstrated natural antigen activity in vitro. The antibody induced by rVP2 subunit vaccine could protect chickens from challenges of IBDV strains, both BC 6/85 and JZ 3/02. The vaccine, in which the VP2 AGP titre is 1:4, would be enough to protect SPF chickens of 19-day-old, but seemed to be relatively lower to protect commercial Avian Broilers under 10-day-age. In field study, Avian Broilers were vaccinated with rVP2 subunit vaccine of 1:16 AGP titre at the age of 7 days. The protection rate was varied from 72% to 95% in different chicken farms. To study the method of serological evaluation, antibody respond to vaccination had been detected with three kinds of tests. The correct ratio of detection decreased in the order of VP2-based ELISA, AGP test, and virions-based ELISA, if the result of IBDV detection was used as standard of judging. Correlation coefficient between the OD values of VP2-based ELISA and the virions-based ELISA was 0.782. The results will make it possible for the vaccine to be produced commercially and used in poultry industry in large scale.


Subject(s)
Birnaviridae Infections/prevention & control , Birnaviridae Infections/veterinary , Chickens/immunology , Infectious bursal disease virus/immunology , Poultry Diseases/prevention & control , Viral Structural Proteins/immunology , Viral Vaccines/pharmacology , Animals , Antibodies, Viral/immunology , Birnaviridae Infections/genetics , Birnaviridae Infections/immunology , Chickens/virology , Enzyme-Linked Immunosorbent Assay , Escherichia coli/genetics , Infectious bursal disease virus/genetics , Poultry Diseases/genetics , Poultry Diseases/immunology , Recombinant Proteins/genetics , Recombinant Proteins/immunology , Recombinant Proteins/pharmacology , Vaccines, Synthetic/genetics , Vaccines, Synthetic/immunology , Vaccines, Synthetic/pharmacology , Viral Structural Proteins/genetics , Viral Vaccines/immunology
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