Simultaneous nitriles degradation and bioflocculant production by immobilized K. oxytoca strain in a continuous flow reactor.

High cost is one of the limiting factors in the industrial production of bioflocculant. Simultaneous preparation of bioflocculant from the contaminants in wastewater was considered as a potential approach to reduce the production cost. In this study, butyronitrile and succinonitrile were verified as sole nitrogen sources for the growth of strain K. oxytoca GS-4-08 in batch experiments. Moreover, more than 90 % of the mixed nitriles could be degraded in a continuous flow reactor, and the bioflocculant could be prepared simultaneously in the effluent. All the as-prepared bioflocculants exhibited high flocculation efficiencies of over 90 % toward Kaolin solution. FTIR and XPS results further unveiled that, the bioflocculant samples with abundance of carboxyl, amine and hydroxyl groups may play an important role on adsorption of Pd2+. The adsorption process could be well simulated by Freundlich model, and the Kf values were as high as 452.8 mg1-1/n l1/n g-1. The results obtained in this study not only confirm the technical feasibility for preparation of bioflocculant from various single nitrile and/or mixed nitriles, but also promise its economic feasibility.

Preparation of a bioflocculant by using acetonitrile as sole nitrogen source and its application in heavy metals removal.

A novel bioflocculant, A-GS408, produced by Klebsiella oxytoca GS-4-08 cultured in acetonitrile (ACN) as sole nitrogen source was investigated in this study. A complete degradation of 1 g l-1 of ACN was achieved in 350 h, and 4.6 g of crude A-GS408 can be obtained in one litter of synthetic medium. The as-prepared bioflocculant exhibits good flocculation efficiency (over 90%) toward Kaolin solution with the aid of Fe3+. Chemical analysis showed that the bioflocculant was mainly composed of polysaccharides (46.3%) and proteins (20.6%). FTIR and XPS results indicated the abundant carboxyl, amine and hydroxyl groups in A-GS408 s, which play an important role on Pd2+ and Cu2+ adsorption. The adsorption of heavy metals can be well stimulated by Freundlich isotherm equation, and the Kf was up to 439.2 mg1-1/n l1/n g-1 and 112.2 mg1-1/n l1/n g-1 for Pb2+ and Cu2+, respectively. The kinetic fitting results proved that the adsorption of heavy metals by A-GS408 was chemisorption. This study may provide a new method for preparation of bioflocculant, which can not only degrade toxic compound i.e., acetonitrile, but also can reuse considerable nitrogen source from nitrile-containing wastewater.

Bioreduction of azo dyes was enhanced by in-situ biogenic palladium nanoparticles.

Biogenic nanoparticles are promising materials for their green synthesis method and good performance in stimulation on reduction of environmental contaminants. In this study, Pd(0) nanoparticles (bio-Pd) were generated by Klebsiella oxytoca GS-4-08 in fermentative condition and in-situ improved the azo dye reduction. The bio-Pd was mainly located on cell membrane with a size range of 5-20 nm by TEM and XRD data analyses. Anthraquinone-2-disulfonate (AQS) greatly increased the reduction rate of Pd(II) with a reduction efficiency as high as 96.54 ±â€¯0.23% in 24 h. The quinone respiration theory, glucose metabolism and the biohydrogen pathway were used to explain the enhancement mechanism of the in-situ generated bio-Pd on azo dye reduction. These results indicate that the in-situ generated bio-Pd by K. oxytoca strain is efficient for azo dye reduction without complex preparation processes, which is of great significance for the removal and subsequent safe disposal of hazardous environmental compounds.