A Controlled Clinical Study of Accelerated High-Dose Theta Burst Stimulation in Patients with Obsessive-Compulsive Disorder.

Background: Obsessive-compulsive disorder (OCD) is frequently treated using a combination of counseling, drugs, and, more recently various transcranial stimulation protocols, but all require several weeks to months for clinically significant improvement, so there is a need for treatments with faster onset. This study investigated whether an accelerated high-dose theta burst stimulation (ahTBS) protocol significantly improves the efficacy of OCD compared to traditional 1-Hz repetitive transcranial magnetic stimulation (rTMS) in the routine clinical setting. Method: Forty-five patients with OCD were randomized into two groups and treated with ahTBS or 1-Hz rTMS for 5 days. Patients were assessed at baseline at the end of treatment using the Yale-Brown Obsessive-Compulsive Scale (Y-BOCS). Results: After 5 days of treatment, there was a significant decrease in Y-BOCS scores in both groups (p < 0.001), and the difference between the two groups was not statistically significant (group × time interaction, F = 1.90, p=0.18). There was also no statistically significant difference in other secondary outcome indicators, including depression, anxiety symptoms, and response rate. However, the ahTBS group had a greater trend in response rate. Neuropsychological testing showed no negative cognitive side effects of either treatment. Conclusion: Accelerated high-dose TBS is as safe and has comparable short-term efficacy to traditional 1-Hz rTMS for the clinical treatment of OCD. Further research is needed to explore optimal ahTBS parameters, validate the utility of this treatment modality, and identify factors predictive of rapid clinical response to guide clinical decision-making. This trial is registered with NCT05221632.

Isolation of cadmium-resistant microbial strains and their immobilisation of cadmium in soil.

Six cadmium (Cd)-resistant microbial strains were isolated and their ability to immobilise Cd2+ in soil investigated. Cd-1, Cd-2, Cd-5, and Cd-6 were identified as Stenotrophomonas sp., Cd-3 as Achromobacter sp., and Cd-7 as Staphylococcus sp. The six strains showed a wide adaptation range for salinity and a strong tolerance to Cd2+. The effects of the initial Cd2+ concentration (1-100 mg/L), duration (18-72 h), temperature (10-40 °C), and pH (5.0-9.0) on the efficiency of Cd2+ removal were analysed. The results revealed that the Cd2+ removal rate was higher at an initial Cd2+ concentration of 5-100 mg/L than at 1 mg/L. The maximum Cd2+ removal effect was at a culture duration of 36 h, temperature of 10-35 °C, and pH of 5.0-7.0. X-ray diffraction (XRD) analysis revealed that the Cd2+ was immobilised by Stenotrophomonas sp. Cd-2 and Staphylococcus sp. Cd-7 through bio-precipitation. X-ray photoelectron spectroscopy (XPS) revealed that the Cd2+ was adsorbed by Stenotrophomonas sp. Cd-2, Achromobacter sp. Cd-3, and Staphylococcus sp. Cd-7. Fourier transform infrared spectroscopy (FTIR) analysis revealed that the isolates reacted with the Cd2+ mainly through the O-H, protein N-H, C-N, lipid C-H, fatty acid COO, polysaccharide C-O, P-O, and other functional groups, as well as with lipid molecules on the cell wall surfaces. Scanning electron microscopy (SEM) analysis revealed that there was little difference in the cells after Cd2+ treatment. The results of the soil remediation experiments indicated that the toxicity of Cd in soil could be effectively reduced using certain strains of microbe.

Preparation and Characterization of Intracellular and Exopolysaccharides during Cycle Cultivation of Spirulina platensis.

The dried cell weight (DCW) of Spirulina platensis gradually decreased from 1.52 g/L to 1.18 g/L after five cultivation cycles. Intracellular polysaccharide (IPS) and exopolysaccharide (EPS) content both increased with increased cycle number and duration. IPS content was higher than EPS content. Maximum IPS yield (60.61 mg/g) using thermal high-pressure homogenization was achieved after three homogenization cycles at 60 MPa and an S/I ratio of 1:30. IPS showed a more fibrous, porous, and looser structure, and had a higher glucose content and Mw (272.85 kDa) compared with EPS, which may be indicative of IPS's higher viscosity and water holding capacity. Although both carbohydrates were acidic, EPS had stronger acidity and thermal stability than IPS; this was accompanied by differences in monosaccharide. IPS exhibited the highest DPPH (EC50 = 1.77 mg/mL) and ABTS (EC50 = 0.12 mg/mL) radical scavenging capacity, in line with IPS's higher total phenol content, while simultaneously showing the lowest HO• scavenging and ferrous ion chelating capacities; thus characterizing IPS as a superior antioxidant and EPS as a stronger metal ion chelator.

Nutritional risk screening in malignant tumors: a study of 375 cancer inpatients.

Malnutrition is a common complication in cancer patients. It often accelerates disease progression and affects treatment outcomes. Thus, in the early census of cancer patients, examination for possible nutritional risks and correcting potential causes of malnutrition are needed to improve patients' quality of life. Our study included 375 patients diagnosed with cancer in Henan province and analyzed the relationship between nutritional risk and indicators like age, serum albumin, serum prealbumin, serum hemoglobin, tumor stage, tumor type, and inflammatory factors. We found that age, hemoglobin, and presence of gastrointestinal tumors were independent risk factors for nutritional risk. We also found significant correlation between inflammatory factors and nutritional risk in cancer patients, so as to provide new prediction indexes for clinical management of nutritional risk and dynamic changes of nutritional status.

Mechanism of bio-electrokinetic remediation of pyrene contaminated soil: Effects of an electric field on the degradation pathway and microbial metabolic processes.

In this study, the mechanism of bio-electrokinetic (BIO-EK) remediation to improve the degradation of pyrene was evaluated based on an analysis of the intermediate products and the microbial community. The results show that BIO-EK remediation has a higher pyrene degradation efficiency on pyrene and its intermediate products than the bioremediation and electrokinetic (EK) remediation processes. A series of intermediate products were detected. According to the type of the intermediate products, two degradation pathways, biological metabolism and electrochemical oxidation, are proposed in the BIO-EK remediation of pyrene. Furthermore, the primary microbial taxa involved in the pollutant degradation changed, which led to variations in the functional gene components. The abundant and functional genes related to metabolism were specifically analyzed. The results indicate that the electric field promotes the expression of metabolisms associated with 14 carbohydrates, 13 lipids, 13 amino acids, five energies, and in particular, 11 xenobiotics. These results suggest that in addition to the promotion effect on the microbial metabolism caused by the electric field, BIO-EK remediation can promote the degradation of pollutants due to the coexistence of a microbial metabolic pathway and an electrochemical oxidation pathway.

The anti-cancer effect of metoclopramide on triple-negative breast cancer cells.

Triple-negative breast cancer (TNBC) is the most aggressive type of breast cancers. Chemotherapy is the most important therapeutic option for TNBC, and chemotherapy-induced nausea and vomiting (CINV) is inevitable. Metoclopramide is a good and cost-effective therapeutic option for chemotherapy-induced nausea and vomiting. However, it is not commonly used in breast cancer because it can increase serum prolactin levels by blocking dopamine D2 receptor. This study aimed at elucidating the effect of metoclopramide on triple-negative breast cancer, MDA-MB-231 cells were treated with various concentrations of metoclopramide, the cell proliferation was detected by MTT method, the apoptosis rate was detected by Annexin V/PI double staining method, the expression change of death-related protein was detected by Western Blot. We found that metoclopramide inhibits cell proliferation and induces cell apoptosis of MDA-MB-231 in a concentration-dependent manner, and the Bcl family was involved in this process.

Microbial community responses to soil parameters and their effects on petroleum degradation during bio-electrokinetic remediation.

This study evaluated the interactions among total petroleum hydrocarbons (TPH), soil parameters, and microbial communities during the bio-electrokinetic (BIO-EK) remediation process. The study was conducted on a petroleum-contaminated saline-alkali soil inoculated with petroleum-degrading bacteria with a high saline-alkali resistance. The results showed that the degradation of TPH was better explained by second-order kinetics, and the efficacy and sustainability of the BIO-EK were closely related to soil micro-environmental factors and microbial community structures. During a 98-d remediation process, the removal rate of TPH was highest in the first 35 d, and then decreased gradually in the later period, which was concurrent with changes in the soil physicochemical properties (conductivity, inorganic ions, pH, moisture, and temperature) and subsequent shifts in the microbial community structures. According to the redundancy analysis (RDA), TPH, soil temperature, and electric conductivity, as well as SO42-, Cl-, and K+ played a better role in explaining the changes in the microbial community at 0-21 d. However, pH and NO3- better explained the changes in the microbial community at 63-98 d. In particular, the dominant genera, Marinobacter and Bacillus, showed a positive correlation with TPH, conductivity, and SO42-, Cl-, and K+, but a negative relationship with pH and NO3. Rhodococcus was positively correlated with soil temperature. The efficacy and sustainability of the BIO-EK remediation process is likely to be improved by controlling these properties.

Hydrothermal Synthesis and Electrochemical Properties of Spherical α-MnO2 for Supercapacitors.

In the present work, spherical α-MnO2 with a high specific capacitance was synthesized by a two-step hydrothermal route. MnCO3 precursors were first prepared by a common hydrothermal method, and then converted to α-MnO2 via a hydrothermal reaction between the precursors and KMnO4 solutions. The effects of hydrothermal temperature on the morphology, crystal structure and specific area of the MnO2 were investigated by scanning electron microscopy (SEM), X-ray diffraction (XRD) and BET measurements. The electrochemical capacitive properties of the manganese dioxides with different morphologies and structures were evaluated by cyclic voltammetry and galvonostatic charge-discharge tests. The results showed that the temperature in the second hydrothermal step had prominent impact on the capacitive properties of a-MnO2. The MnO2 synthesized at 150 *C exhibited a highest specific capacitance of 328.4 Fx g(-1) at a charge-discharge current density of 100 mA x g(-1).

Synergistic effects of bioremediation and electrokinetics in the remediation of petroleum-contaminated soil.

The present study evaluated the coupling interactions between bioremediation (BIO) and electrokinetics (EK) in the remediation of total petroleum hydrocarbons (TPH) by using bio-electrokinetics (BIO-EK) with a rotatory 2-D electric field. The results demonstrated an obvious positive correlation between the degradation extents of TPH and electric intensity both in the EK and BIO-EK tests. The use of BIO-EK showed a significant improvement in degradation of TPH as compared to BIO or EK alone. The actual degradation curve in BIO-EK tests fitted well with the simulated curve obtained by combining the degradation curves in BIO- and EK-only tests during the first 60 d, indicating a superimposed effect of biological degradation and electrochemical stimulation. The synergistic effect was particularly expressed during the later phase of the experiment, concurrent with changes in the microbial community structure. The community composition changed mainly according to the duration of the electric field, leading to a reduction in diversity. No significant spatial shifts in microbial community composition and bacterial numbers were detected among different sampling positions. Soil pH was uniform during the experimental process, soil temperature showed no variations between the soil chambers with and without an electric field.