ABSTRACT
A novel ergostane, sarocladione (1), was isolated from the deep-sea-derived fungus Sarocladium kiliense, along with 20 known compounds. The structure of 1 was determined mainly by a detailed analysis of its experimental and calculated NMR spectroscopic data. It is worth noting that 1 was the first steroid bearing a 5,10:8,9-diseco moiety. All 21 compounds were tested for in vitro antitumor activities against five cancer cell lines. ß-Sitostenone (7) and 4,6-dihydroxyeudesmane (20) showed significant effects on HeLa-S3 cells with the IC50 values of 9.2 µM and 9.3 µM, respectively.
Subject(s)
Acremonium/chemistry , Antineoplastic Agents/pharmacology , Secosteroids/pharmacology , Steroids/pharmacology , Antineoplastic Agents/chemistry , Antineoplastic Agents/isolation & purification , Dose-Response Relationship, Drug , Drug Screening Assays, Antitumor , HeLa Cells , Humans , Molecular Conformation , Secosteroids/chemistry , Secosteroids/isolation & purification , Steroids/chemistry , Steroids/isolation & purification , Structure-Activity RelationshipABSTRACT
The isolation and structure elucidation of six new prenylated acylphloroglucinols, faberiones A-F, from the whole plant of Hypericum faberi is reported. Faberiones A-D (1-4) share a rare styrene substituent and may be biosynthetically generated via further acylation of the acylphloroglucinols. By analyzing the MS and NMR data, the structures of the new isolates were established. Faberiones B (2) and C (3) showed moderate cytotoxicity against the pancreatic cell line (PANC-1) with IC50 values of 6.2 and 9.0 µM, respectively.
