Identification and antimicrobial properties of a new alkaloid produced by marine-derived Verrucosispora sp. FIM06-0036.

The actinomycete strain FIM06-0036 was isolated from marine sponge sample collected from the East China Sea and identified as Verrucosispora sp. based upon the results of 16S rRNA sequence analysis. One new alkaloid, 2-ethylhexyl 1H-imidazole-4-carboxylate (1), together with a known alkaloid butyl 1H-imidazole-4-carboxylate (2) was obtained from the fermentation products of this strain, the structures of compounds 1 and 2 were determined by their detailed analysis of 1 D, 2 D NMR and HR-ESI-MS data, along with literature data analysis. Compounds 1 and 2 were evaluated for their antimicrobial activity with MIC (minimum inhibitory concentration) values ranging from 8 to 256 µg · mL-1 against Helicobacter pylori, Klebsiella Pneumonia, Staphylococcus aureus and Enterococcus faecalis.

[Qualitative and quantitative analysis of alkaloids in Eurycoma longifolia by HPLC-Q-TOF-MS combined with UPLC-QQQ-MS/MS].

A sensitive and efficient method was established and validated for qualitative and quantitative analysis of total alkaloids from the extract of Eurycoma longifolia by high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(HPLC-Q-TOF-MS) combined with ultra-performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry(UPLC-QQQ-MS/MS). The HPLC-Q-TOF-MS conditions are as follows: Welch Ultimate XB-C_(18) column(4.6 mm×250 mm, 5 µm) with acetonitrile(containing 0.1% formic acid)-0.1% formic acid in water as mobile phase for gradient elution. The UPLC-QQQ-MS/MS conditions are as below: Agilent Eclipse Plus C_(18) column(2.1 mm×50 mm, 1.8 µm) with acetonitrile(containing 0.1% formic acid) and 0.1% formic acid in water as mobile phase for gradient elution. MS data were collected by electrospray ionization in positive ion mode. According to the comparison with reference standards and the accurate masses of molecules, a total of 17 alkaloids in E. longifolia extract were identified by HPLC-Q-TOF-MS. The UPLC-QQQ-MS/MS quantitative analysis result of 3 alkaloids showed that the linear ranges of them were good(r≥0.999 7) and the overall recoveries ranged from 108.8%-110.2%, with RSDs of 2.9%-5.3%. The method is accurate, reliable, and efficient, which can comprehensively reflect the constituents and content of alkaloids in E. longifolia. The result can serve as a reference for further elucidating its therapeutic material basis and quality control.

Isolation, purification and identification of two new alkaloids metabolites from marine-derived Verrucosispora sp. FIM06025.

Chemical investigation of a marine-derived actinomycete strain Verrucosispora sp. FIM06025 isolated from a marine sponge sample collected from the East China Sea, resulted in the discovery of two new alkaloids, (2-(hydroxymethyl)-3-(2-(hydroxymethyl)-3-methylaziridin-1-yl) (2-hydroxyphenyl) methanone (1) and 2-(1-hydroxyethyl)-3,4-dihydrobenzo [f] [1,4]oxazepin-5(2H)-one (2). The structures of compounds 1 and 2 were determined by the detailed analysis of 1D, 2D NMR and HR-TOF-MS data, along with literature data analysis. The bioefficacy investigations revealed that compound 1 exhibited a broad spectrum of antimicrobial activity with MIC (minimum inhibitory concentration) values ranging from 3.4 to 200 µg·mL-1 against H. pylori, P. aeroginosa, A. baumanniiin, E. coli and K. pneumonia, S. aureus, C. albicans and E. faecium, however, compound 2, up to 200 µg/mL, displayed no antibacterial activity against these bacteria.

Structure elucidation and antitumour activity of a new macrolactam produced by marine-derived actinomycete Micromonospora sp. FIM05328.

Chemical investigation of a marine-derived actinomycete strain Micromonospora sp. FIM05328 isolated from a soil sample collected from the East China Sea, resulted in the discovery of a new 26-membered polyene macrolactam metabolite FW05328-1 (1), together with a known polyene with pyridone ring compound aurodox (2). The structures of compounds 1 and 2 were determined by the detailed analysis of 1D, 2D NMR and HR-TOF-MS data, along with literature data analysis. 1 and 2 exhibited excellent antiproliferative activities against KYSE30, KYSE180 and EC109 human tumour cell lines, but displayed no antibacterial activities against bacteria or fungi were tested.

FW523-3, a novel lipopeptide compound, induces apoptosis in cancer cells.

FW523-3, a new lipopeptide compound, was recently isolated and purified from the culture broth of a marine Micromonospora chalcea. FW523-3 was shown to inhibit the proliferation of certain cancer cells. However, the spectra and the underlying mechanism of its antitumor activity are unclear. In this study, the MTT and colony formation assays were employed to determine the antitumor spectra of FW523-3 and its effect on cell proliferation, respectively. Apoptosis was analyzed using DNA laddering assay and flow cytometry and the involved pathways were explored by Western blotting. Results revealed that FW523-3 exhibited cytotoxicity in a panel of tumor cell lines including esophageal squamous cell carcinoma cells (EC109), lung cancer cells (A549 and 95D), gastric cancer cells (SGC7901), uterine cervix cancer cells (HeLa) and hepatocellular carcinoma cells (HepG2). Based on these results, FW523-3 inhibited the colony formation ability of tumor cells. Moreover, FW523-3 induced apoptosis via activation of caspases 9, 7 and 3. FW523-3 also blocked the ERK and p38 signaling pathways. Taken together, we propose that FW523-3 acts as a broad-spectrum antitumor drug. FW523-3 inhibits tumor cell growth and induces tumor cell apoptosis via the mitochondrial and MAPK pathways.

[Impact of childhood abuses on the psychology and behaviors regarding harmful dietary pattern in adolescents].

OBJECTIVE: To examine the effect of childhood abuses on adolescents' psychology and behaviors related to harmful dietary pattern. METHODS: Anonymous questionnaire study on childhood abuses, adolescents' psychology and behaviors regarding their dietary patterns was conducted among 5141 students in 9 middle schools in 2 areas of Anhui province. RESULTS: Among 5141 students, 29.9% reported having severe childhood physical abuse, 64.8% having intermediate childhood physical abuse, 51.4% having mental abuse, 5.3% having physical contact sexual abuse and 24.5% having non-physical contact sexual abuse. In junior, senior middle schools and vocational schools, the incidence rate of severe childhood physical abuse, physical contact sexual abuse and non-physical contact sexual abuse among male students was higher than that among female students. In total, the incidence rate of childhood mental abuse among female students (53.1%) was higher than that among male students (49.8%) and with significant difference (chi2 = 5.484, P < 0.05). The incidence rate of 5 kinds of childhood abuses among junior middle school students was relatively low, and the incidence rate of intermediate childhood physical abuse and mental abuse was higher among senior middle school students. The incidence rate of 9 among the 11 kinds of psychology and behaviors related to harmful dietary pattern among female students in the middle schools and vocational schools was higher than that among male students. By unconditional multivariate logistic regression model, results showed that the childhood abuses was among the 11 kinds of psychological and behavioral risk factors related to harmful dietary pattern. CONCLUSION: Childhood abuses had negative effects on dietary behaviors in adolescents.