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1.
Zhonghua Yi Xue Za Zhi ; 85(38): 2667-73, 2005 Oct 12.
Article in Chinese | MEDLINE | ID: mdl-16324290

ABSTRACT

OBJECTIVE: In partial loss of distal finger segment, a corresponding part of the toe tissue compound is harvested and transplanted for repair or reconstruction. This new procedure gives forth a new concept and is called decorative repair or reconstruction. METHODS: In a series of 77 patients with 88 thumb and/or finger subtotal defects in forms of lateral half, dorsal half or volar half composite tissue defects were reconstructed with lateral nail-skin flap, dorsal skin-nail flap or pulp flap taken from corresponding part of the toes. The blood circulations were re-established by anastomosing digital arteries of the toe transplants and fingers. RESULTS: In this series 75 patients with 78 fingers reconstructed are successful. The overall survival rate is 97.5%. Follow-up examinations made half to 12 years postoperatively showed the fingers are having a normal length, outward appearance and function. There are nails preserved. The pulps are full. Sweating function are present. Two-point-discrimination tests are between 4-6 mm. CONCLUSION: By decorative reconstruction of subtotal dorsal, lateral, or volar halves defect of thumb and/or fingers by transplanting corresponding part of soft tissue taken from the toe has the merit of repair of any parts of tissue loss precisely what is needed. This procedure is better than any traditional toe-to-hand transfer and realizing the exact meaning of decorative reconstruction.


Subject(s)
Finger Injuries/surgery , Surgical Flaps , Thumb/injuries , Adolescent , Adult , Child , Child, Preschool , Female , Follow-Up Studies , Humans , Male , Plastic Surgery Procedures/methods , Thumb/surgery , Toes/blood supply , Toes/transplantation , Transplantation, Autologous , Young Adult
2.
Guang Pu Xue Yu Guang Pu Fen Xi ; 24(3): 327-9, 2004 Mar.
Article in Chinese | MEDLINE | ID: mdl-15759989

ABSTRACT

A mixed complex of La with 2,2-bipyridyl (dpy) and 1,10-phenanthroline(phen) was synthesized. Elemental analysis, IR and TGA of the complex were investigated, thus it was proved that the conformation of it was La(dpy)(phen)Cl3. The interaction of the complex with DNA has been studied also. The spectra showed that the interaction between La(dpy)(phen)Cl3 and DNA experienced intercalation of long-range self-assembly. The study made it clear that the synthesized mixed complex La(dpy)(phen)Cl3 can interact with DNA, and La(dpy)(phen)Cl3 can be used for probing a nucleic acid.


Subject(s)
2,2'-Dipyridyl/chemistry , DNA/chemistry , Lanthanum/chemistry , Nucleic Acid Conformation/drug effects , Phenanthrolines/chemistry , 2,2'-Dipyridyl/pharmacology , Circular Dichroism/methods , Lanthanum/pharmacology , Phenanthrolines/pharmacology , Photochemistry
3.
Guang Pu Xue Yu Guang Pu Fen Xi ; 22(4): 631-3, 2002 Aug.
Article in Chinese | MEDLINE | ID: mdl-12938383

ABSTRACT

A highly sensitive fluorimetry determination of DNA is developed in this paper. The maximum fluorescence intensity was produced in the pH 3.05, with the maximum excitation and emission wavelength of 362 and 531 nm, respectively. Under the optimum conditions, the fluorescence intensity was proportional to the concentration of DNA. The linear range for calf thymus DNA is of 0-120 mg.L-1. The limit of determination was 0.07 mg.L-1. When the proposed method was used to determine DNA in the presence of some coexisting substances, a satisfactory result was obtained.


Subject(s)
Berberine/chemistry , DNA/analysis , Spectrometry, Fluorescence/methods , DNA/chemistry , Flow Injection Analysis/methods
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