Enhancement of anaerobic digestion of rice straw by amino acid-derived ionic liquid.

This study proposes a novel method to enhance methane production from anaerobic digestion using an amino acid-derived ionic liquid, glycine hydrochloride, ([Gly][Cl]), as an exogenous additive. After 40 days of digestion with 5% [Gly][Cl], the cumulative methane production was 115.56 mL/g VS, which was 73% higher than that of the control group (without additive). Specifically, the peak activities of cellulase, xylanase, and lignin peroxidase were significantly higher than those of the control group. The addition of [Gly][Cl] increased bacterial diversity and reduced archaeal diversity. Synergistota represented by Syner-01, Fibrobacterota represented by BBMC-4, Bacteroides, and unclassified_f__Lachnospiraceae significantly increased in relative abundance. It suggested that [Gly][Cl] stimulated the activities of protein-hydrolyzing and acid-producing bacteria. [Gly][Cl] also increased the abundance of methanogens and archaea, converting more lignocellulose to methane. Methanobacterium, that metabolizes H2 and CO2 to CH4, was more abundant. Therefore, [Gly][Cl] can improve methane yield as an anaerobic digestion additive.

Food waste digestate composting enhancement by sodium polyacrylate addition: Effects on nitrogen transformation processes and bacterial community dynamics.

The influences of sodium polyacrylate (PAAS) at the ratios of 0% (CK), 0.5% (F1), 1.0% (F2), 1.5% (F3), 2.0% (F4) and 2.5% (F5) on nitrogen transformation and bacterial community composition were investigated in the composting of food waste digestate (FWD) and corn straw (CS). PAAS addition increased the thermophilic temperature but had no significant effect on pH values. PAAS exerted significantly effects on the concentration of total nitrogen (TN), ammonia nitrogen (NH4+-N), nitrite-nitrogen (NO2--N) and nitrate-nitrogen (NO3--N). The compost product in 1.0% PAAS treatment was more active in absorbing nutrients. Firmicutes (9.40-83.54%), Actinobacteriota (9.98-51.50%), Proteobacteria (0.20-27.87%) and Bacteroidota (0.11-34.69%) were the dominant phyla in FWD composting. Moreover, relative to CK, PAAS promoted the propagation of dominant bacterial phyla Firmicutes with increment of 30.05-102.06% in the thermophilic phase. Kroppenstedtia, Thermobifida and Saccharomonospora were observed to be dominant at the maturing phase and correlated with NH4+-N, NO2--N, TN and NO3--N. Therefore, they might be regarded as probable biomarkers symbolic for the maturing phase during FWD composting. The compost product had the highest maturity degree in 1.0% PAAS treatment. These results indicated that PAAS addition improved the maturity and nutrient contents of the compost product as well as altered compost bacterial community dynamics.

Isoprene Production on Enzymatic Hydrolysate of Peanut Hull Using Different Pretreatment Methods.

The present study is about the use of peanut hull for isoprene production. In this study, two pretreatment methods, hydrogen peroxide-acetic acid (HPAC) and popping, were employed prior to enzymatic hydrolysis, which could destroy the lignocellulosic structure and accordingly improve the efficiency of enzymatic hydrolysis. It is proven that the isoprene production on enzymatic hydrolysate with HPAC pretreatment is about 1.9-fold higher than that of popping pretreatment. Moreover, through High Performance Liquid Chromatography (HPLC) analysis, the amount and category of inhibitors such as formic acid, acetic acid, and HMF were assayed and were varied in different enzymatic hydrolysates, which may be the reason leading to a decrease in isoprene production during fermentation. To further increase the isoprene yield, the enzymatic hydrolysate of HPAC was detoxified by activated carbon. As a result, using the detoxified enzymatic hydrolysate as the carbon source, the engineered strain YJM21 could accumulate 297.5 mg/L isoprene, which accounted for about 90% of isoprene production by YJM21 fermented on pure glucose (338.6 mg/L). This work is thought to be the first attempt on isoprene production by E. coli using peanut hull as the feedstock. More importantly, it also shows the prospect of peanut hull to be considered as an alternative feedstock for bio-based chemicals or biofuels production due to its easy access and high polysaccharide content.