ABSTRACT
OBJECTIVE: To investigate the clinical significance of the three-step approach in laparoscopic hemihepatectomy. METHODS: A total of 32 patients received laparoscopic hemihepatectomy with the three-step approach in Yijishan Hospital affiliated to Wannan Medical College between Aug 2013 and Oct 2015. All patients underwent thin slice CT scan and hemihepatectomy was imitated with the imagic explorer, preoperatively. The vessel distribution was observed at the section and the three-step approach was used in the hemihepatectomy. Pre- and post-operative data were collected and analyzed retrospectively. RESULTS: The length of middle hepatic vein (MHV) was (59.1±12.9) mm and the number of branchs to the left and right lobe were 3.07±0.78 and 3.11±0.64 respectively. The distance between the first branch of MHV and the diaphragmatic surface was (28.07±3.74) mm and the distance between MHV and the visceral surface was (14.4±4.3) mm. The laparoscopic surgeries (left hemihepatectomy in 28 and right hemihepatectomy in 4) were performed successfully in all cases with the three-step approach, without any conversion to the open surgeries. The operation time was (165±42) min in left hemihepatectomy and (305±50) min in right hemihepatectomy. The intraoperative blood loss was (242±65) ml in left hemihepatectomy and (695±122)ml in right hemihepatectomy. All the patients recovered well without severe complications except for bile leakage in 3 patients who were cured with drainage. The postoperative hospital stay was (7.96±1.8) d. CONCLUSIONS: the distribution of vessel is regional at the section of hemihepatectomy and the three-step approach based on this feature is safe and effective in laparoscopic hemihepatectomy, which can shorten the operation time and reduce the difficulty of operation.
Subject(s)
Hepatectomy/methods , Hepatic Veins/diagnostic imaging , Laparoscopy/methods , Liver/surgery , Blood Loss, Surgical , Hepatectomy/instrumentation , Humans , Laparoscopy/instrumentation , Length of Stay , Liver/diagnostic imaging , Retrospective Studies , Treatment OutcomeABSTRACT
To investigate the effects of autophagy activator and autophagy inhibitor on the CNE2 radiation sensitivity of nasopharyngeal carcinoma cells. RNA interference technology was used to silence the atg5 gene and autophagy inhibition cell model was constructed. Rapamycin and chloroquine were treated respectively on cells with X-ray 5Gy irradiation. Cells' growth status were observed for 8 days and control group was set. The cell viability was detected by MTT assay and colony formation assay, and the cell cycle was analyzed by flow cytometry. Compared with the control group, the survival rate, clone formation rate and the survival rate of the irradiation of the other three groups were significantly lower. (P<0.05) Most cells were detected in the G0/G1 phase in the other three groups except the control group, and cells of the other two periods were less than those in the G0/G1 phase. The autophagy inhibitor or activator and atg5 silencing can be increased by CNE2 radiation therapy, however, the sensitization effect increase of autophagy activator is better than others.
Subject(s)
Autophagy/radiation effects , Gamma Rays , Radiation Tolerance/radiation effects , Autophagy/drug effects , Autophagy-Related Protein 5/antagonists & inhibitors , Autophagy-Related Protein 5/genetics , Autophagy-Related Protein 5/metabolism , Cell Line, Tumor , Cell Survival/drug effects , Chloroquine/toxicity , Flow Cytometry , G1 Phase Cell Cycle Checkpoints/drug effects , G1 Phase Cell Cycle Checkpoints/radiation effects , Humans , RNA Interference , RNA, Small Interfering/metabolism , Radiation Tolerance/drug effects , Sirolimus/toxicityABSTRACT
Excellent photoconductive properties have been found in Sb(2)O(3) nanobelts synthesized by a surfactant-assisted solvothermal method. Visible-light photodetectors have been designed from Sb(2)O(3) nanobelt networks using micrometer-wide gold wires as masks. Photodetectors show high sensitivity to visible light, high stability, and reproducibility. Fast response and decay times (<0.3 s) are comparable or even better than these parameters in many other metal oxide nanoscale photodetectors. The dominant mechanism of excellent photoconductivity is attributed to the barrier height modulations in the nanobelt-to-nanobelt contact regions. These results demonstrate that Sb(2)O(3) nanobelt networks can indeed serve as high-performance photodetectors in the visible light range.
Subject(s)
Nanostructures/chemistry , Nanostructures/ultrastructure , Photometry/instrumentation , Tin Compounds/chemistry , Equipment Design , Equipment Failure Analysis , Light , Nanostructures/radiation effects , Tin Compounds/radiation effectsABSTRACT
We report the growth of ultrathin single-crystal ZnO nanobelts by using a Ag-catalyzed vapor transport method. Extensive transmission electron microscopy and atomic force microscopy measurements reveal that the thickness of the ultrathin ZnO nanobelts is approximately 2 nm. Scanning electron microscopy and post-growth annealing studies suggest a '1D branching and 2D filling' growth process. Our results demonstrate the critical role of catalyst in the deterministic synthesis of nanomaterials with the desired morphology. In addition, these ultrafine nanobelts exhibit stable field emission with unprecedented high emission current density of 40.17 mA cm(-2). These bottom-up building blocks of ultrathin ZnO nanobelts may facilitate the construction of advanced electronic and photonic nanodevices.
ABSTRACT
The field emission properties of SnO(2) nanowires fabricated by chemical vapor deposition with metallic catalyst-assistance were investigated. For the as-fabricated SnO(2) nanowires, the turn-on and threshold field were 4.03 and 5.4 V/mum, respectively. Considerable enhancement of field emission of SnO(2) nanowires was obtained by a post-annealing process in oxygen at high temperature. When the SnO(2) nanowires were post-annealed at 1,000 degrees C in oxygen, the turn-on and threshold field were decreased to 3.77 and 4.4 V/mum, respectively, and the current density was increased to 6.58 from 0.3 mA/cm(2) at the same applied electric field of 5.0 V/mum.
ABSTRACT
An immunosensor for measuring antigen-antibody binding by nanogold particles enhancement was present here. A gold plate electrode was modified by a SAM (self-assembled monolayer) of 11-Mercaptoundecanioc Acid. Then sheep anti-rabbit IgG was immobilized onto the end of the 11-Mercaptoundecanioc Acid. With that the gold electrode was exposed to the mixed solution of rabbit IgG and nanogold labeled sheep anti-rabbit IgG. The differential capacitance of the electrical double layer was monitored to detect the antigen-antibody reaction. The result showed the capacity of the electrical double layer by nanogold enhancement was largely increased compared to that in traditional capacitive immunosensor. The detection sensitivity is up to 10nF / μg/ml.
ABSTRACT
Hexagonal cadmium selenide (CdSe) nanowires, with diameter around 20 nm, were synthesized using a simple vapor-phase growth. Silicon (Si) powder acts as a source material assisting the synthesis, which is very important to the formation of the CdSe nanowires. We also suggest that self-catalysis at the Cd-terminated (0001) surface, together with the assistance action of Si, leads to the formation of wire-like structures to be formed. Meanwhile, the assistance of Si is responsible for the fineness and uniformity of the CdSe nanowires. The possible growth mechanism of the CdSe nanowires is proposed, and the optical property of the as-grown CdSe nanowires is characterized.
Subject(s)
Cadmium Compounds/chemistry , Nanotechnology , Selenium Compounds/chemistry , Silicon/chemistry , Crystallization , Microscopy, Electron, ScanningABSTRACT
AIM: To compare the pharmacokinetic characteristics of four preparations of fluorouracil (5-FU) administered rectally using a rat model. METHODS: Concentrations of 5-FU were measured in plasma, the rectal wall and mesentery lymph tissues of rats and rabbits by high performance liquid chromatography. Differences between the main pharmacokinetic parameters were compared by statistical analysis. RESULTS: The 5-FU concentrations in the rectal wall and mesenteric lymph tissues were significantly higher than the concentration in blood following rectal administration for all four of the preparations (P < 0.01). The drug level in the rectal wall was higher in the animals received delivery of an emulsion, compared to those who received delivery as a suppository (P < 0.05). Moreover, the animals who received a lipophil-based suppository had lower plasma level of drug than those who received a hydrophil-based suppository, and the animals who received the simple (o/w) emulsion had lower plasma level than those who received the complex (w/o/w) emulsion. The differences found in the rat model were confirmed in rabbits (P < 0.01). CONCLUSION: The lipophil-based suppository and the simple emulsion of 5-FU might be more suitable for rectal administration for treatment of rectal cancers.
