ABSTRACT
An increasing number of cases of novel coronavirus pneumonia (NCP) infected with 2019-nCoV have been identified in Wuhan and other cities in China, since December 2019. We analyzed data on the 17 confirmed cases in Dazhou to provide the epidemiologic characteristics of NCP outside Wuhan. Among them, 12 patients were still quarantined in the hospital, 5 patients were discharged NCP patients according to the national standards. Compared with non-discharged NCP patients, the discharged NCP patients had younger ages. Moreover, discharged NCP patients had higher heart rate, lymphocytes levels and monocytes levels than non-discharged NCP patients on admission to the hospital. Notably, all of 17 patients had abnormal increased C-reactive protein levels, and 16 patients had abnormal computed tomography images. This study provided some information that younger age, higher lymphocytes levels and monocytes levels at the diagnoses of 2019-nCoV may contributed to faster recovery and better therapeutic outcome.
ABSTRACT
Objective To investigate the application of fluorimetric reverse transcriptase-polymerase chain reaction(RT-PCR) to estimate postmortem interval(PMI)in rats.Methods A total of 44 rats were killed by cervical dislocation and placed in a chamber with temperature-controlling system at 20℃ for 0 to 8 d postmortem.Changes of the relative content of glyceraldehyde phosphate dehydrogenase(GAPDH) mRNA in the brain and liver were examined by fluorimetric RT-PCR.Results The amplification of GAPDH mRNA could be detected within 7 d in the brain and 2 d in the liver postmortem respectively,in a fashion of decreasing.Conclusion Fluorimetric RT-PCR can detect the time-dependent degradation of GAPDH mRNA in the brain and the liver,so it maybe provide a new index for estimating PMI.
ABSTRACT
Objective To study changes in expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA in the kidney and spleen of dead rats and its application in deducing the postmortem intervals (PMI), so as to look for a new method for PMI deduction. Method 28 rats were killed by cervical dislocation, placed in a chamber with temperature-controlling system at 20℃ for 0 to 48h postmortem, and autopsied at 0, 8, 16, 24, 32, 40 and 48h respectively (n=4 for each time point). The expression of GAPDH mRNA in kidney and spleen was assessed with fluorescence semi-quantitative RT-PCR. Results The amplified products of GAPDH mRNA could be detected in kidney within 40h and in spleen within 32h postmortem respectively, and they showed a decreasing tendency as its fluorescence intensity declined gradually and lost at last. Conclusion Detection of GAPDH mRNA expression in kidney and spleen of rats at different periods after death by fluorescence semi-quantitative RT-PCR seems to be of some value for estimation of PMI.
