Virulence factors, serogroups, and antibiotic resistance of Shiga-toxin producing Escherichia coli from raw beef, chicken meat, and vegetables in Southwest Iran.

BACKGROUND: Shiga-toxin-producing Escherichia coli (STEC) is an important food-borne pathogen causing human diseases with severe symptoms. Although the O157 serotype has been mostly isolated from human specimens, the increasing incidence rates of non-O157 serogroups have attracted special attention in recent years. AIMS: Evaluation of the epidemiology and identification of different characteristics of STEC isolates from raw beef, chicken meat, and vegetable samples in Shiraz, Southwest Iran. METHODS: Two hundred beef and chicken meat samples from different parts of carcasses and four hundred vegetable samples (carrots, lettuce, cucumber, and leafy greens) were randomly taken; STEC were isolated and confirmed using standard microbiological methods. Antimicrobial susceptibility testing (AST) was performed using the Kirby-Bauer disc diffusion method. Polymerase chain reaction (PCR) was used for the identification of O-serogroups, virulence, and antibiotic resistance genes. RESULTS: 52% of beef, 8% of chicken, and 7.2% of vegetable samples were STEC-positive. Further, the highest frequency of virulence factors belonged to the co-existence of stx1 and stx2. O157 serogroup was only detected in beef (3.8%) and lettuce (16.6%) isolates, while the rates of the non-O157 serogroups were relatively high (up to 44.2%). The highest resistance rate in the STEC isolates of different samples belonged to nalidixic acid (62.5%), tetracycline (55.7%), and ampicillin (48%). CONCLUSION: Paying more attention to non-O157 serogroups in future studies is recommended due to the relatively high prevalence of theses STEC serogroups in our study. Besides, the high level of resistance to some antibiotics observed in this study needs to be addressed.

Serogroups, and drug resistance of nontyphoidal Salmonella in symptomatic patients with community-acquired diarrhea and chicken meat samples in Tehran.

BACKGROUND: Salmonella is considered as a main cause of community-acquired diarrhea in humans, however, sources of the multi-drug resistant (MDR) strains and their link with the disease are not well known. AIMS: This study aimed to investigate the frequency, serogroup diversity, and antimicrobial susceptibility patterns of Salmonella strains in poultry meat and stool samples of patients with community acquired diarrhea in Tehran. METHODS: We compared the frequency of non-typhoidal Salmonella serogroups, the similarities of their resistance patterns to 10 antimicrobial compounds, the prevalence of extended spectrum ß-lactamase (ESBL) and ampicillinase C (AmpC) genetic determinants, and class 1 and 2 integrons in 100 chicken meat and 400 stool samples of symptomatic patients in Tehran during June 2018 to March 2019. RESULTS: Salmonella was isolated from 75% and 5.5% of the chicken meats and human stool samples, respectively. The chicken meat isolates mainly belonged to serogroup C (88%, 66/75), while the human stool isolates were mainly related to serogroup D (59.1%, 13/22). The MDR phenotype and the most common rates of resistance to antibiotics, including tetracycline, trimethoprim/sulfamethoxazole (TS) and azithromycin, were detected in 4.5% and 45.3%, 59% and 13.6%, 43% and 9.1%, 42% and 9.1% of the human stool and chicken meat samples, respectively. Carriage of bla CTX, bla SHV, and bla PER genes in the meat isolate with ESBL resistance phenotype and bla ACC, bla FOX, and bla CMY-2 among the 7 meat strains with AmpC resistance phenotype was not confirmed using polymerase chain reaction (PCR). High prevalence of class 1 and 2 integrons was characterized and showed a correlation with resistance to TS and chloramphenicol. CONCLUSION: These findings showed a lack of association between chicken meats and human isolates due to discrepancy between the characterized serogroups and resistance phenotypes.

Prevalence, antimicrobial resistance, and virulence-associated genes of Campylobacter isolates from raw chicken meat in Shiraz, Iran.

BACKGROUND: Campylobacter is recognized as a major cause of foodborne gastroenteritis in humans in many countries and may be transferred from animals to humans. The consumption of chicken meat is identified as a major cause of Campylobacter infection in humans. AIMS: To find out the contamination rate of chicken meat with Campylobacter, the antimicrobial resistance (AMR) pattern, and the virulence-associated genes of the isolates. METHODS: Ninety packed chicken meat from 7 main poultry slaughterhouses in Shiraz were analyzed for Campylobacter spp. isolation through microbiological methods. Specific primers were used for the identification of the Campylobacter isolates on species level by polymerase chain reaction (PCR). Antibiotic resistant profiles were determined using the disc diffusion method based on Clinical and Laboratory Standards Institute (CLSI) standards. All the isolates were screened for 7 virulence-associated genes, namely cdtA, cdtB, cdtC, cadF, pldA, cgtB, and virB11 by PCR. RESULTS: Out of 90 chicken meats, 26 (28.9%) Campylobacter spp. have been isolated. Resistance to ciprofloxacin (CIP), nalidixic acid (NA), and cefixime (CFM) was observed in all the isolates. Resistance to trimethoprim/sulfamethoxazole (SXT), tetracycline (TET), ampicillin (AMP), and chloramphenicol (CHO) was 80.8%, 88.5%, 76.9%, and 30.8%, respectively. Multidrug resistance (MDR) phenotype was observed in 80.8% of the Campylobacter isolates. All the isolates were positive for cdtA, cdtB, cdtC, and cadF genes. pldA and cgtB were detected in 65.4% and 15.4% of the isolates, respectively. CONCLUSION: In this study, the presence of several virulence genes and an alarming level of MDR in Campylobacter spp. isolates were reported. Particularly, resistance to CIP and TET should be highlighted, since both are key drugs for the treatment of human campylobacteriosis.

Feasibility assessment of CT-based thermometry for temperature monitoring during thermal procedure: Influence of ROI size and scan setting on metrological properties.

Computed tomography (CT) thermometry belongs to the wide class of non-invasive temperature monitoring techniques, which includes ultrasound and Magnetic Resonance thermometry. Non-invasive techniques are particularly attractive to be used in hyperthermal procedures for their ability to produce a three-dimensional temperature map and because they overcome the risks related to the insertion of sensing elements.

CT-based thermometry: an overview.

The dependence of computed tomography (CT) values on temperature has been pointed out by several authors since the late 1970s. They emphasised the importance of this phenomenon on the calibration process with water equivalent phantoms of the CT scanners. Few years later the potential of CT thermometry for non-invasive temperature mapping during thermal procedures was investigated. The interest on the employment of this technique during thermal treatments has been recently renewed with the improvement of modern CT scanner performances and with the increased popularity of minimally invasive thermal techniques for cancer treatment. A good thermometry allows avoiding unintended damage of the healthy tissues during the procedure by providing a detailed tissue temperature distribution; therefore, it is recommended in order to achieve good effectiveness of the thermal treatment. Researchers have been working on this issue for more than four decades and different non-invasive solutions have been proposed, i.e., microwave thermal imaging, infrared (IR)-, ultrasound-, magnetic-resonance (MR)-, and CT-based thermometry. This review aims to summarise the essential physics and the currently available data on CT-based thermometry and to elucidate the potential use of this technique during thermal procedures. Background information on measuring principle, an investigation of the performances achieved by this technique and the thermal sensitivity of the CT-number of different organs are provided and discussed.

[Proposal of a computerized method to assess work related stress according to legal requirements in Italy]. / Aggiornamento delle Linee di indirizzo della Regione Toscana sullo stress lavoro-correlato. Realizzazione di un software per l'applicazione facilitata del modello di valutazione proposto alla luce delle indicazioni tecniche del documento di coordinamento tecnico delle regioni.

Assessing psychosocial factors in occupational environment requires complicated procedures according to most methods. The project of Tuscany region has the aim to realize a free computerized instrument for a gradual risk assessment with two different modes, expert and beginner. A decisional flow chart helps to approach the risk assessment step by step on the basis of company features, management and organization problems and company symptoms of stress. This experience means a proposal of a computerized method for a quantitative assessment of psychosocial risks at workplace, but at the same time offers a detailed analysis of the problems in the organization suggesting different priority to solve them.

The Effect of Aqueous Garlic Extract on Interleukin-12 and 10 Levels in Leishmania major (MRHO/IR/75/ER) Infected Macrophages.

BACKGROUND: The aim of the present study was to investigate the immunomodulation effects of aqueous garlic extract (AGE) in the cultured macrophages infected by Leishmania major. METHODS: After J774 macrophages proliferation in RPMI1640 and incubation with Leishmania for 72 hours, AGE was added in doses of 9.25, 18.5, 37, 74 and 148 mg/ml for 18, 24 and 48 hours and cell culture supernatants were harvested. The Leishmania infected J774 cells to assess the cell viability was examined using trypan blue and methylthiazol tetrazolium assay (MTT). An enzyme-linked immunosorbent assay (ELISA) was performed on cell culture supernatants for measurement of interleukin IL-10 and IL-12. RESULTS: Dose of 37 mg/ml for 48 hours of garlic extract was the most potent dose for activation of amastigotes infected macrophages. In addition, AGE increased the level of IL-12 in Leishmania infected cell lines significantly. CONCLUSIONS: AGE treated cell is effective against parasitic pathogens, and AGE induced IL-12 differentially affected the immune response to invading Leishmania parasites.

[Risk assessment method for psychosocial factors at workplace: a North-West Tuscany area project]. / Metodologia di valutazione dei rischi psicosociali in ambiente di lavoro: un progetto toscano di area vasta.

The rising awareness of psychosocial risks at workplace means that it is ever more important to prepare methods to assess psychosocial factors in occupational environment. This project of north west tuscany area has the aim to realize an instrument for a gradual risk assessment for this kind of factors without the support of specialists. A decisional flow chart helps to approach the risk assessment step by step on the basis of company features, management and organization problems and company symptoms of stress. The final assessment combines the evaluation of perceived risks with job analysis realizing a matrix containing 5 risk levels, which suggests the priority of preventive measures. The intermediate level (level three) represents the activation level for the medical surveillance. This experience means a proposal for a quantitative assessment of psychosocial risks at workplace.