Lipopolysaccharide induced MAP kinase activation in RAW 264.7 cells attenuated by cerium oxide nanoparticles.

High mortality rates are associated with the life threatening disease of sepsis. Improvements in septic patient survivability have failed to materialize with currently available treatments. This article represents data regarding a study published in biomaterials (Vellaisamy et al., Biomaterials, 2015, in press). with the purpose of evaluating whether severe sepsis mortality and associated hepatic dysfunction induced by lipopolysaccharide (LPS) can be prevented by cerium oxide nanoparticles (CeO2NPs) treatment in male Sprague Dawley rats. Here we provide the information about the method and processing of raw data related to our study publish in Biomaterials and Data in Brief (Vellaisamy et al., Biomaterials, 2015, in press; Vellaisamy et al., Data in Brief, 2015, in press.). The data contained in this article evaluates the contribution of MAPK signaling in LPS induced sepsis. Macrophage cells (RAW 264.7) were treated with a range of cerium oxide nanoparticle concentration in the presence and absence of LPS. Immunoblotting was performed on the cell lysates to evaluate the effect of cerium oxide nanoparticle treatment on LPS induced changes in Mitogen Activated Protein Kinases (MAPK) p-38, ERK 1/2, and SAPK/JNK phosphorylation.

Cerium oxide nanoparticles inhibit lipopolysaccharide induced MAP kinase/NF-kB mediated severe sepsis.

The life threatening disease of sepsis is associated with high mortality. Septic patient survivability with currently available treatments has failed to improve. The purpose of this study was to evaluate whether lipopolysaccharide (LPS) induced sepsis mortality and associated hepatic dysfunction can be prevented by cerium oxide nanoparticles (CeO2NPs) treatment in male Sprague Dawley rats. Here we provide the information about the methods processing of raw data related to our study published in Biomaterials (Selvaraj et al., Biomaterials, 2015, In press) and Data in Brief (Selvaraj et al., Data in Brief, 2015, In Press). The data present here provides confirmation of cerium oxide nanoparticle treatments ability to prevent the LPS induced sepsis associated changes in physiological, blood cell count, inflammatory protein and growth factors in vivo. In vitro assays investigation the treated of macrophages cells with different concentrations of cerium oxide nanoparticle demonstrate that concentration of cerium oxide nanoparticles below 1 µg/ml did not significantly influence cell survival as determined by the MTT assay.

Effect of cerium oxide nanoparticles on sepsis induced mortality and NF-κB signaling in cultured macrophages.

AIM: To investigate whether cerium oxide (CeO2) nanoparticles could be used for the treatment of severe sepsis. MATERIALS & METHODS: Cecal peritonitis was induced in male Sprague-Dawley rats in the presence and absence of CeO2 nanoparticles. Cultured macrophages (RAW264.7 cells) were challenged with lipopolysaccharide in the absence and presence of CeO2 nanoparticles. The effect of nanoparticles on the growth of Escherichia coli and Staphylococcus aureus was determined in culture. RESULTS: Nanoparticle treatment decreased sepsis-induced mortality, organ damage, serum IL-6, blood urea nitrogen and inflammatory markers. Nanoparticle treatment diminished lipopolysaccharide-induced cytokine release and p65-nuclear factor-KB (NF-KB) activation in cultured RAW264.7 cells. Exposure to CeO2 nanoparticles inhibited E. coli growth. CONCLUSION: The findings of this study indicate that CeO2 nanoparticles may be useful for the treatment of sepsis.