ABSTRACT
BACKGROUND: The plasminogen activator inhibitor type-1 (PAI-1) has been implicated in the regulation of fibrinolysis and extracellular matrix components. The single base pair guanine insertion/deletion polymorphism (4G/5G) within the promoter region of the PAI-1 gene influences PAI-1 synthesis and may modulate hepatic fibrogenesis. AIM: To evaluate the influence of PAI-1 serum levels and 4G/5G polymorphism on the risk of liver fibrosis associated to non-alcoholic fatty liver disease (NAFLD) in morbidly obese patients. MATERIAL AND METHODS: Case-control study of 50 obese patients undergoing bariatric surgery and 71 non-obese subjects matched by age and sex. Anthropometric and biochemical measurements were performed, including PAI-1 serum levels. Genomic DNA was obtained to assess the presence of 4G/5G polymorphism. RESULTS: BMI, insulinemia, triglycerides, HOMA-IR, hypertension and diabetes were significantly higher in obese patients compared to control subjects. PAI-1 serum levels observed in obese patients were significantly lower (10.63 ± 4.82) compared to controls (14.26 ± 11.4; p < 0.05). No differences were observed in the PAI-1 4G/5G promoter genotypes frequencies (p = 0.12). No differences were observed in PAI-1 plasma levels among obese patients with liver fibrosis (10.64 ± 4.35) compared to patients without liver fibrosis (10.61 ± 5.2; p = 0.985). PAI-1 4G/5G promoter genotypes frequencies were similar in patients with or without liver fibrosis associated to NASH (p = 0.6). CONCLUSIONS: Morbidly obese patients had significantly lower PAI-1 serum levels with similar PAI-1 4G/5G genotypes frequencies compared to non-obese subjects. The frequency of 4G/5G genotypes in Chilean Hispanic healthy subjects was similar to that described in other populations. No association was found between PAI-1 serum levels or 4G/5G genotype with liver fibrosis in obese patients.
Subject(s)
Fatty Liver/genetics , Liver Cirrhosis/genetics , Obesity, Morbid/genetics , Plasminogen Activator Inhibitor 1/genetics , Polymorphism, Single Nucleotide , Adult , Bariatric Surgery , Biomarkers/blood , Case-Control Studies , Chi-Square Distribution , Chile/epidemiology , Fatty Liver/blood , Fatty Liver/ethnology , Fatty Liver/pathology , Female , Gene Frequency , Genetic Predisposition to Disease , Humans , Liver/pathology , Liver Cirrhosis/blood , Liver Cirrhosis/ethnology , Liver Cirrhosis/pathology , Logistic Models , Male , Middle Aged , Non-alcoholic Fatty Liver Disease , Obesity, Morbid/blood , Obesity, Morbid/ethnology , Obesity, Morbid/surgery , Odds Ratio , Phenotype , Plasminogen Activator Inhibitor 1/blood , Promoter Regions, Genetic , Risk Assessment , Risk Factors , Severity of Illness IndexABSTRACT
Objetivos: Avaliar a influência do frênulo da língua curto na falade indivíduos com fissura labio palatina, definir a necessidade de intervenção cirúrgica para tal aspecto. Métodos: No presente trabalho foram avaliados 21 indivíduos com fissura labio palatina e frênulo da língua curto, operados de lábio e palato, sem intervenção cirúrgica no frênulo da língua, com idades entre 7 e 50 anos, de ambos os gêneros, regularmente matriculados no Hospital de Reabilitação de Anomalias Cranio faciais (HRAC), da Universidade de São Paulo, Campus de Bauru. Foram descartados da amostra casos que apresentavam fístulasno palato, outras mal formações associadas, síndromes, déficit cognitivo, perda auditiva neuros sensorial. Resultados: Os achados mostraram: nenhum dos casos apresentou queixas quanto à alimentação;19,0 por cento de queixa de fala todas relacionadas à articulação; 33,1 por cento de queixas quanto habilidades específicas que envolvem movimento da língua, com maior freqüência para lamber sorvete...
Objectives: To evaluate the effect of short lingual frenulum in the speech of individuals with cleft lip and palate. To define the need for surgical intervention in such cases. Methods: In the present study, 21 individuals with short lingual frenulum as identified during oral examination, who underwent lip and palate surgery, without lingual frenulum intervention were evaluated by an experienced Speech-Language Pathologist. The subjects were over 7 years of age, of both genders, and enrolled for treatment at the Hospital for Rehabilitation of Cranio facial Anomalies (HRAC) of the University of Sao Paulo Bauru Campus. Those individuals with palate fistulae, other associated malformations, syndromes, cognitive deficiencies, or neurosensorial hearing loss were not included in the sample. Results: Findings revealed that: none of the cases presented complaints as to feeding or swallowing disorders, 33.1 per cent presented complaints regarding specific abilities concerning tongue movement, with the highest frequency found for difficulties to lick ice cream...
