Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 4 de 4
Filter
Add more filters










Language
Publication year range
1.
Rev Bras Parasitol Vet ; 23(1): 16-22, 2014 Mar.
Article in English | MEDLINE | ID: mdl-24728356

ABSTRACT

The general aim of this study, which was conducted for the first time in Iran, was to evaluate the seroprevalence and geographical distribution of Ehrlichia canis in a dog population in Iran, followed by molecular confirmation using PCR and sequencing. Blood samples were collected from 240 dogs in different areas of Alborz and Tehran Provinces and initially analyzed using the immunofluorescent antibody (IFA) test to detect anti-Ehrlichia canis IgG antibodies. Subsequently, nested PCR was performed based on a fragment of the 16S rRNA gene of E. canis on serologically positive samples. The results showed that 40/240 dogs (16.6%) presented anti-Ehrlichia canis IgG antibodies and that nine of the blood samples from the 40 seropositive dogs (22.5%) contained E. canis DNA, which was confirmed by sequencing. The seroprevalence of E. canis tended to be higher in purebred, one to three-year-old male dogs living in the Plain zone, in rural areas; however, this difference was not statistically significant.


Subject(s)
Antibodies, Bacterial/blood , Dog Diseases/epidemiology , Dog Diseases/microbiology , Ehrlichia canis/immunology , Ehrlichiosis/veterinary , Animals , Dog Diseases/blood , Dogs , Ehrlichia canis/isolation & purification , Ehrlichiosis/blood , Ehrlichiosis/epidemiology , Female , Iran/epidemiology , Male , Risk Factors , Seroepidemiologic Studies
2.
Rev. bras. parasitol. vet ; 23(1): 16-22, Jan-Mar/2014. tab, graf
Article in English | LILACS | ID: lil-707189

ABSTRACT

The general aim of this study, which was conducted for the first time in Iran, was to evaluate the seroprevalence and geographical distribution of Ehrlichia canis in a dog population in Iran, followed by molecular confirmation using PCR and sequencing. Blood samples were collected from 240 dogs in different areas of Alborz and Tehran Provinces and initially analyzed using the immunofluorescent antibody (IFA) test to detect anti-Ehrlichia canis IgG antibodies. Subsequently, nested PCR was performed based on a fragment of the 16S rRNA gene of E. canis on serologically positive samples. The results showed that 40/240 dogs (16.6%) presented anti-Ehrlichia canis IgG antibodies and that nine of the blood samples from the 40 seropositive dogs (22.5%) contained E. canis DNA, which was confirmed by sequencing. The seroprevalence of E. canis tended to be higher in purebred, one to three-year-old male dogs living in the Plain zone, in rural areas; however, this difference was not statistically significant.


O objetivo geral deste estudo, que foi feito pela primeira vez no Irã, foi avaliar a soroprevalência e distribuição geográfica de Ehrlichia canis em população de cães no Irã, seguida da confirmação molecular por meio de PCR seguida de sequenciamento. Amostras de sangue de 240 cães de diferentes áreas das Províncias de Alborz e Teerã foram coletadas e, inicialmente, analisadas pelo Reação de Imunofluorescência (IFA) para detecção de anticorpos IgG anti-Ehrlichia canis Subsequentemente, uma reação do tipo nested PCR baseada em um fragmento do gene 16S rRNA de E. canis foi realizada nas amostras sorologicamente positivas. Os resultados mostraram que 40/240 cães (16,6%) apresentaram anticorpos IgG anti- Ehrlichia canis e nove (22,5%) das amostras de sangue dos 40 cães soropositivos continham DNA de E. canis, confirmado por sequenciamento. A soroprevalência de E. canis, embora não estatisticamente significativa, mostrou uma tendência em se apresentar maior em cães machos com 1-3 anos, de raça pura, que vivem em zonas planas e áreas rurais.


Subject(s)
Animals , Dogs , Female , Male , Antibodies, Bacterial/blood , Dog Diseases/epidemiology , Dog Diseases/microbiology , Ehrlichia canis/immunology , Ehrlichiosis/veterinary , Dog Diseases/blood , Ehrlichia canis/isolation & purification , Ehrlichiosis/blood , Ehrlichiosis/epidemiology , Iran/epidemiology , Risk Factors , Seroepidemiologic Studies
3.
Zoo Biol ; 33(3): 221-7, 2014.
Article in English | MEDLINE | ID: mdl-24535989

ABSTRACT

Different species of chironomids larvae (Diptera: Chironomidae) so-called bloodworms are widely distributed in the sediments of all types of freshwater habitats and considered as an important food source for amphibians. In our study, three species of Chironomidae (Baeotendipes noctivagus, Benthalia dissidens, and Chironomus riparius) were identified in 23 samples of larvae from Belgium, Poland, Russia, and Ukraine provided by a distributor in Belgium. We evaluated the suitability of these samples as amphibian food based on four different aspects: the likelihood of amphibian pathogens spreading, risk of heavy metal accumulation in amphibians, nutritive value, and risk of spreading of zoonotic bacteria (Salmonella, Campylobacter, and ESBL producing Enterobacteriaceae). We found neither zoonotic bacteria nor the amphibian pathogens Ranavirus and Batrachochytrium dendrobatidis in these samples. Our data showed that among the five heavy metals tested (Hg, Cu, Cd, Pb, and Zn), the excess level of Pb in two samples and low content of Zn in four samples implicated potential risk of Pb accumulation and Zn inadequacy. Proximate nutritional analysis revealed that, chironomidae larvae are consistently high in protein but more variable in lipid content. Accordingly, variations in the lipid: protein ratio can affect the amount and pathway of energy supply to the amphibians. Our study indicated although environmentally-collected chironomids larvae may not be vectors of specific pathogens, they can be associated with nutritional imbalances and may also result in Pb bioaccumulation and Zn inadequacy in amphibians. Chironomidae larvae may thus not be recommended as single diet item for amphibians.


Subject(s)
Amphibians/physiology , Animal Feed/analysis , Aquatic Organisms/physiology , Chironomidae/chemistry , Chironomidae/microbiology , Chironomidae/virology , Diet/veterinary , Animal Feed/microbiology , Animal Feed/standards , Animal Feed/virology , Animals , Animals, Zoo/physiology , Larva , Metals, Heavy/analysis , Nutrition Assessment
SELECTION OF CITATIONS
SEARCH DETAIL
...