Integrative Analysis of Long Isoform Sequencing and Functional Data Identifies Distinct Cortical Layer Neuronal Subtypes Derived from Human iPSCs.

Generation of human induced pluripotent stem cells (iPSCs) through reprogramming was a transformational change in the field of regenerative medicine that led to new possibilities for drug discovery and cell replacement therapy. Several protocols have been established to differentiate hiPSCs into neuronal lineages. However, low differentiation efficiency is one of the major drawbacks of these approaches. Here, we compared the efficiency of two methods of neuronal differentiation from iPSCs cultured in two different culture media, StemFlex Medium (SFM) and Essential 8 Medium (E8M). The results indicated that iPSCs cultured in E8M efficiently generated different types of neurons in a shorter time and without the growth of undifferentiated non-neuronal cells in the culture as compared to those generated from iPSCs in SFM. Furthermore, these neurons were validated as functional units immunocytochemically by confirming the expression of mature neuronal markers (i.e., NeuN, Beta tubulin, and Synapsin I), and whole-cell patch-clamp recordings. Long-read single-cell RNA sequencing confirms the presence of upper and deep layer cortical layer excitatory and inhibitory neuronal subtypes in addition to small populations of GABAergic neurons in day 30 neuronal cultures. Pathway analysis indicated that our protocol triggers the signaling transcriptional networks important for the process of neuronal differentiation in vivo.

Comparison of Phototherapy Effect with and without Phenobarbital on the Newborns with Hyperbilirubinemia.

Objectives: Jaundice occurs in 60% of full-term and 80% of pre-term newborns. This study compared the effect of phototherapy with and without phenobarbital on icteric newborns. Materials & Methods: This study is a randomized clinical trial conducted from July until March 2018 at Imam Reza Hospital, Mashhad University of Medical Science, Iran. Full-term and near-term neonates with more than 2000 grams who were hospitalized in the mentioned period for jaundice were entered into the study. The newborns were divided into two groups using block randomization. Data were analyzed by SPSS version 19. Results: The average gestational age was 36.4 weeks (SD 2.39) in the intervention group and 36.9 weeks (SD 2.16) in the control group, with no significant difference between them. The mean hospital stay for the intervention group was 72 hours (SD 1.66), compared to 55 hours (SD 1.88) for the control group. At discharge, the serum bilirubin level in the intervention group was 11.53 mg/dL (SD 0.77), while it was 10.80 mg/dL (SD 1.09) in the control group, a statistically significant difference. Conclusion: According to this study, phototherapy with phenobarbital is not more effective than phototherapy alone in neonatal hyperbilirubinemia.

Topological Sholl descriptors for neuronal clustering and classification.

Neuronal morphology is a fundamental factor influencing information processing within neurons and networks. Dendritic morphology in particular can widely vary among cell classes, brain regions, and animal species. Thus, accurate quantitative descriptions allowing classification of large sets of neurons is essential for their structural and functional characterization. Current robust and unbiased computational methods that characterize groups of neurons are scarce. In this work, we introduce a novel technique to study dendritic morphology, complementing and advancing many of the existing techniques. Our approach is to conceptualize the notion of a Sholl descriptor and associate, for each morphological feature, and to each neuron, a function of the radial distance from the soma, taking values in a metric space. Functional distances give rise to pseudo-metrics on sets of neurons which are then used to perform the two distinct tasks of clustering and classification. To illustrate the use of Sholl descriptors, four datasets were retrieved from the large public repository https://neuromorpho.org/ comprising neuronal reconstructions from different species and brain regions. Sholl descriptors were subsequently computed, and standard clustering methods enhanced with detection and metric learning algorithms were then used to objectively cluster and classify each dataset. Importantly, our descriptors outperformed conventional morphometric techniques (L-Measure metrics) in several of the tested datasets. Therefore, we offer a novel and effective approach to the analysis of diverse neuronal cell types, and provide a toolkit for researchers to cluster and classify neurons.

Retrospective analysis of direct antiglobulin test positivity at tertiary academic hospital over 10 years.

BACKGROUND: Hemolytic disease of the fetus and newborn (HDFN) is a clinically significant problem that may potentially affect any pregnancy. Direct antiglobulin test (DAT) is considered to be an important test in identifying newborns who are suspected to have HDN. This study aims in reviewing data regarding a positive DAT result concerning etiology and the development of HDN over a period of 10 years. STUDY DESIGN AND METHODS: A retrospective study of all neonates with a positive DAT result between January 2011 and December 2020 was performed. Data were obtained from patients' electronic hospital files, transfusion medicine databases, and medical birth records. Laboratory parameters along with clinical interventions in neonates with a DAT-positive result and a comparison group of DAT-negative neonates were performed. RESULTS: 36,000 deliveries were registered in this period. 176 (2.65 %) neonates had a positive DAT result. ABO-incompatibility was the most common cause with 59.1 %; Rh incompatibility 13.8 %, minor blood group incompatibility, and other RBC-related antibodies 10.1 %, and unspecified etiology in 17 % of cases. Among DAT-positive cases, 32.7 % of neonates were diagnosed with HDN. ABO-incompatibility was the major reason as well. Initial mean total bilirubin levels were higher in the DAT-positive group than the control group (p < 0.001), and these neonates also had a lower initial hemoglobin level (p < 0.001). The need for therapeutic interventions was significantly higher in DAT-positive neonates (p < 0.001) as 86.8 % underwent phototherapy, with 32.7 %, and 17.6 % receiving exchange transfusion (ET) and intravenous immunoglobulin (IVIG), respectively. CONCLUSION: In conclusion, ABO incompatibility was the most common cause for neonatal DAT positivity. Besides the common causes of DAT positivity, there would be rare but important conditions that may lead to a positive result, such as antibodies passively acquired from mothers in the context of alloimmunizations or using drugs. In addition, as a high rate of therapeutic intervention was identified among neonates with a DAT-positive result, there is a crucial need for increasing awareness regarding early diagnosis of the condition, careful monitoring, and the employment of prenatal alloimmunization screening tests.

Developmental Changes in Pyramidal Cell Morphology in Multiple Visual Cortical Areas Using Cluster Analysis.

Neuronal morphology is characterized by salient features such as complex axonal and dendritic arbors. In the mammalian brain, variations in dendritic morphology among cell classes, brain regions, and animal species are thought to underlie known differences in neuronal function. In this work, we obtained a large dataset from http://neuromorpho.org/ comprising layer III pyramidal cells in different cortical areas of the ventral visual pathway (V1, V2, V4, TEO, and TE) of the macaque monkey at different developmental stages. We performed an in depth quantitative analysis of pyramidal cell morphology throughout development in an effort to determine which aspects mature early in development and which features require a protracted period of maturation. We were also interested in establishing if developmental changes in morphological features occur simultaneously or hierarchically in multiple visual cortical areas. We addressed these questions by performing principal component analysis (PCA) and hierarchical clustering analysis on relevant morphological features. Our analysis indicates that the maturation of pyramidal cell morphology is largely based on early development of topological features in most visual cortical areas. Moreover, the maturation of pyramidal cell morphology in V1, V2, V4, TEO, and TE is characterized by unique developmental trajectories.

OxCOVID19 Database, a multimodal data repository for better understanding the global impact of COVID-19.

Oxford COVID-19 Database (OxCOVID19 Database) is a comprehensive source of information related to the COVID-19 pandemic. This relational database contains time-series data on epidemiology, government responses, mobility, weather and more across time and space for all countries at the national level, and for more than 50 countries at the regional level. It is curated from a variety of (wherever available) official sources. Its purpose is to facilitate the analysis of the spread of SARS-CoV-2 virus and to assess the effects of non-pharmaceutical interventions to reduce the impact of the pandemic. Our database is a freely available, daily updated tool that provides unified and granular information across geographical regions. Design type Data integration objective Measurement(s) Coronavirus infectious disease, viral epidemiology Technology type(s) Digital curation Factor types(s) Sample characteristic(s) Homo sapiens.

Meteorological patterns, technical validation, and chemical comparison of atmospheric dust depositions and bulk sand in the Arabian Gulf region.

This study reports seasonal variations of meteorological parameters, atmospheric dust and dust-borne heavy metals concentrations measured, over a period of two years, next to two major airports (Dubai International Airport and Abu Dhabi International Airport) in the Gulf Cooperation Council (GCC) region. On-line monitoring stations were installed at each location next to dust samplers used to frequently collect PM2.5 and PM10 on Teflon filters for metal analysis. Clear seasonal variation in meteorological parameters were identified. The particulate matter concentrations depicted from the two locations were continuously monitored. The PM2.5 concentration ranged from 50 to 100 µg/m3 on normal days but reached 350-400 µg/m3 per day during mild storms. The PM10 levels ranged between 100 and 250 µg/m3 during normal days and spiked to 750 µg/m3 during mild storms. Energy Dispersive X-Ray Analysis (EDS) revealed the presence of significant amounts of alkali and alkaline earth metals, which pose potential harm to aircraft engines. ICP analysis showed the presence of heavy and toxic metals in concentrations that may pose harm to human health. Bulk sand samples from Abu Dhabi sites showed chemical similarities to the atmospheric dust samples. The concentrations of heavy metals, PM2.5, and PM10 are at levels that require further monitoring due to their impact on human health. The two years meteorological monitoring, with the seasonal variations, provided additional regional data in the Arabian Gulf. Furthermore, the study concluded that Sand and Dust storms (SDS) occur more frequently at the northern Arabian Gulf compared to its southern region. The chemical correlation between atmospheric dust and regional desert sand suggests the localized origin of the smaller dust particles that may form by breaking apart of the ground sand grains. As a result of the ongoing urbanization in the region, it is essential to collect additional data from various locations for a longer period of time.

A mechanized device for mounting histological tissue sections.

BACKGROUND: Traditional methods for mounting tissue sections onto slides are suboptimal as the amount of labor required quickly multiplies with increasing number of samples. Methods to accelerate the tissue mounting process while reducing the associated risk of tissue damage are needed. NEW METHOD: We designed and 3D printed a mechanized device with an inclined platform used to mount tissue sections onto slides in buffer solution. The main advantage of this design is to reduce the time required for mounting sections as well as minimize the possibility of damaging delicate or thin tissue sections. RESULTS: Using our device, we illustrate and describe in detail the steps required to mount smaller coronally cut mouse brain sections, as well as bigger tangentially cut ferret brain sections. This method's efficiency was assessed by comparing the time required to mount an entire slide of ferret brain sections using our method and the conventional method. Using our device reduced the tissue mounting time by 60%. COMPARISON WITH EXISTING METHOD(S): Compared to existing conventional tissue mounting methods, our device is a simple and user friendly alternative that substantially reduces the time required to mount tissue sections while preserving tissue section quality. CONCLUSIONS: Using our device can streamline histological processing and prove to be especially useful for a variety of tissue types as the platform was designed to accommodate different size microscope slides, and thus use for varying tissue section sizes.

The association between 25-dehydroxy vitamin D and lower respiratory infection in children aged less than "5" years in Imam Reza hospital, Bojnurd, Iran.

OBJECTIVE: To clarify the association between vitamin D deficiency and acute respiratory infection in children below age 5 years. METHODS: The cross-sectional study was conducted at Imam Reza Hospital in Bojnurd, Iran, in June 2013 and comprised 90 children below 5 years of age suffering from respiratory infections. They was selected on the basis of purposive sampling and were then categorised into two equal groups of 'acute' and 'non-acute' respiratory infection. Data collection was done using a questionnaire and serum level of 25-dehydroxycalcciferol was measured. SPSS 11 was used to analyse and interpret the data. RESULTS: In the group of children with respiratory disorders, 9 (42.9%) exhibited vitamin D deficiency. There were no significant differences between the two groups in terms of demographic characteristics such as age, intrauterine age, weight, birth-weight, head circumference, height, gender, living area and respiratory distress (p>0.05 each). Vitamin D deficiency showed no meaningful statistical relation with acute respiratory infections (p>0.05). CONCLUSIONS: More studies with higher sample size and are recommended.

Ophthalmic, hearing, speaking and school readiness outcomes in low birth weight and normal birth weight primary school children in Mashhad-Iran.

Low Birth weight infants are at risk of many problems. Therefore their outcome must evaluate in different ages especially in school age. In this study we determined prevalence of ophthalmic, hearing, speaking and school readiness problems in children who were born low birth weight and compared them with normal birth weight children. In a cross-sectional and retrospective study, all Primary School children referred to special educational organization center for screening before entrance to school were elected in Mashhad, Iran. In this study 2400 children enrolled to study and were checked for ophthalmic, hearing, speaking and school readiness problems by valid instrument. Data were analyzed by SPSS 11.5. This study showed that 8.3% of our population had birth weight less than 2500 gram. Visual impairment in LBW (Low Birth Weight) and NBW (Normal Birth Weight) was 8.29% vs. 5.74% and there was statistically significant difference between them (P=0.015). Hearing problem in LBW and NBW was 2.1% vs. 1.3 and it was not statistically significant. Speaking problem in LBW and NBW was 2.6% vs. 2.2% and it was not statistically significant. School readiness problem in LBW and NBW was 12.4% vs. 5.8% and it was statistically significant (P<0.001). According to the results, neurological problems in our society is more than other society and pay attention to this problem is critical. We believe that in our country, it is necessary to provide a program to routinely evaluate LBW children.

Noise pollution in intensive care units and emergency wards.

INTRODUCTION: The improvement of technology has increased noise levels in hospital Wards to higher than international standard levels (35-45 dB). Higher noise levels than the maximum level result in patient's instability and dissatisfaction. Moreover, it will have serious negative effects on the staff's health and the quality of their services. The purpose of this survey is to analyze the level of noise in intensive care units and emergency wards of the Imam Reza Teaching Hospital, Mashhad. PROCEDURE: This research was carried out in November 2009 during morning shifts between 7:30 to 12:00. Noise levels were measured 10 times at 30-minute intervals in the nursing stations of 10 wards of the emergency, the intensive care units, and the Nephrology and Kidney Transplant Departments of Imam Reza University Hospital, Mashhad. The noise level in the nursing stations was tested for both the maximum level (Lmax) and the equalizing level (Leq). The research was based on the comparison of equalizing levels (Leq) because maximum levels were unstable. RESULTS: In our survey the average level (Leq) in all wards was much higher than the standard level. The maximum level (Lmax) in most wards was 85-86 dB and just in one measurement in the Internal ICU reached 94 dB. The average level of Leq in all wards was 60.2 dB. In emergency units, it was 62.2 dB, but it was not time related. The highest average level (Leq) was measured at 11:30 AM and the peak was measured in the Nephrology nursing station. CONCLUSION: The average levels of noise in intensive care units and also emergency wards were more than the standard levels and as it is known these wards have vital roles in treatment procedures, so more attention is needed in this area.

Comparison of blood lead levels in 1-7 year old children with and without seizure.

OBJECTIVE: Lead is a nonessential metal and is not a natural constituent in humans. Lead is very toxic for children, especially to the central nervous system. We studied and compared blood lead levels in children aged 1-7 years old with and without convulsions. METHODS: In this study we randomly measured blood lead levels in 206 children referred to Imam Reza Hospital, Pediatric Emergency Ward and Outpatient Clinic, Mashad, Iran from December 2001 to June 2003. There were 95 children with convulsions and 111 without convulsions. RESULTS: There were no significant differences in the mean value of age, place of residence and economic status between the 2 groups (P values were 0.20 for age, 0.14 for place of residence and 0.76 for economic status). The mean blood lead level +/- SD in the convulsive group was 126.53 +/- 35.91 ug/lit and in the non-convulsive group was 118.03 +/- 32.10 ug/lit (p = 0.70). CONCLUSION: This study showed that the blood lead levels in convulsive patients is not statistically significant compared to the non-convulsive group and routine measurement is not advised.