Knowledge and perception of milk producers about thermal stress in Brazilian dairy farms.

Heat stress is a challenge for the milk production chain, especially in tropical environments. Thus, the objective was to evaluate the knowledge and the perception of milk producers about heat stress and determine what corrective measures they adopted to minimize losses in the productive performance of dairy cows due to high temperatures. A questionnaire was applied to 112 dairy farmers in the states of Minas Gerais and Goiás, Brazil in 2019 and 2020. The collected data were submitted to descriptive statistics using the G test of independence and multivariate correspondence analysis. Among the surveyed producers, 89% stated that they had already been informed about what heat stress is and how it affects the animals; 94% declared that they had already noticed in their day-to-day activities that heat stress impairs productivity and milk quality, and 92% stated that they have tried to reduce the heat stress to which the animals in their herd are exposed. There was an association among previous knowledge about heat stress and farm size, daily volume of milk produced, number of lactating cows, type of milking and presence of technical assistance. There was also an association among the adoption of measures that minimize the negative effects of heat stress with the size of the property, the number of cows in lactation and with the producer's perception of heat stress in their daily lives. It was concluded that, in important municipalities of the Brazilian dairy chain, most milk producers surveyed have knowledge and perception of the negative effects of heat stress on the productive performance of dairy cows and sought to adopt measures that could alleviate them.

Novel CaLB-like Lipase Found Using ProspectBIO, a Software for Genome-Based Bioprospection.

Enzymes have been highly demanded in diverse applications such as in the food, pharmaceutical, and industrial fuel sectors. Thus, in silico bioprospecting emerges as an efficient strategy for discovering new enzyme candidates. A new program called ProspectBIO was developed for this purpose as it can find non-annotated sequences by searching for homologs of a model enzyme directly in genomes. Here we describe the ProspectBIO software methodology and the experimental validation by prospecting for novel lipases by sequence homology to Candida antarctica lipase B (CaLB) and conserved motifs. As expected, we observed that the new bioprospecting software could find more sequences (1672) than a conventional similarity-based search in a protein database (733). Additionally, the absence of patent protection was introduced as a criterion resulting in the final selection of a putative lipase-encoding gene from Ustilago hordei (UhL). Expression of UhL in Pichia pastoris resulted in the production of an enzyme with activity towards a tributyrin substrate. The recombinant enzyme activity levels were 4-fold improved when lowering the temperature and increasing methanol concentrations during the induction phase in shake-flask cultures. Protein sequence alignment and structural modeling showed that the recombinant enzyme has high similarity and capability of adjustment to the structure of CaLB. However, amino acid substitutions identified in the active pocket entrance may be responsible for the differences in the substrate specificities of the two enzymes. Thus, the ProspectBIO software allowed the finding of a new promising lipase for biotechnological application without the need for laborious and expensive conventional bioprospecting experimental steps.

Enzymes and pathways in microbial production of 2,3-butanediol and 3-acetoin isomers.

2,3-Butanediol (BD) and acetoin (AC) are products of the non-oxidative metabolism of microorganisms, presenting industrial importance due to their wide range of applications and high market value. Their optical isomers have particular applications, justifying the efforts on the selective bioproduction. Each microorganism produces different isomer mixtures, as a consequence of having different butanediol dehydrogenase (BDH) enzymes. However, the whole scene of the isomer bioproduction, considering the several enzymes and conditions, has not been completely elucidated. Here we show the BDH classification as R, S or meso by bioinformatics analysis uncovering the details of the isomers production. The BDH was compared to diacetyl reductases (DAR) and the new enoyl reductases (ER). We observed that R-BDH is the most singular BDH, while meso and S-BDHs are similar and may be better distinguished through their stereo-selective triad. DAR and ER showed distinct stereo-triads from those described for BDHs, agreeing with kinetic data from the literature and our phylogenetic analysis. The ER family probably has meso-BDH like activity as already demonstrated for a single sequence from this group. These results are of great relevance, as they organize BD producing enzymes, to our known, never shown before in the literature. This review also brings attention to nontraditional enzymes/pathways that can be involved with BD/AC synthesis, as well as oxygen conditions that may lead to the differential production of their isomers. Together, this information can provide helpful orientation for future studies in the field of BD/AC biological production, thus contributing to achieve their production on an industrial scale.

Retrospective study of retroviruses by immunoenzymatic test on cats in Grande Vitória (ES, Brazil) and associated neoplasms.

Retroviruses are among the leading causes of death in domestic cats. Retroviruses associate with the host cell in a persistent and permanent way, leading to diverse clinical conditions. The feline leukemia virus (FeLV) is the most pathogenic retrovirus with the potential to cause both degenerative diseases and immunosuppression, as well as proliferative diseases, as its association with the cell may lead to a direct oncogenic effect. The feline immunodeficiency virus (FIV), in turn, can lead to the classic immunodeficiency syndrome, usually has a chronic, less aggressive course and has no direct oncogenic effect. The use of vaccines and control measures has resulted in a decrease in the prevalence of FeLV in the United States of America (USA) and Europe, however, in Brazil, statistics show prevalence rates above 50%. This study aimed to assess the prevalence of feline retroviruses, by immunoenzymatic assay testing, in the region of Grande Vitória, in Espírito Santo and also point out the frequency of neoplasms in these cats. A total of 388 cats were retrospectively evaluated (2014-2016). The prevalence of FIV was 2.3% and FeLV was 33.7%. Neoplasms were identified in the three cats seropositive for FIV and FeLV and in three cats infected only with FIV. Neoplasms were also found in 26.6% of cats that were seropositive only for FeLV, especially mediastinal lymphoma. The high prevalence of FeLV demonstrated in this study highlights the need for establishing effective control measures, with emphasis on vaccination.

As retroviroses encontram-se entre as principais causas de morte em gatos domésticos. Os retrovírus associam-se à célula hospedeira de forma persistente e permanente, levando a quadros clínicos diversos. O vírus da leucemia felina (FeLV) é oretrovírus mais patogênico, com potencial para ocasionar tanto doenças degenerativas e imunossupressão, quanto doenças proliferativas, pois sua associação à célula pode levar ao efeito oncogênico direto. Já o vírus da imunodeficiência felina (FIV), pode levar à clássica síndrome de imunodeficiência, costuma ter um curso crônico, menos agressivo e não possui efeito oncogênico direto. O uso de vacinas e de medidas de controle resultaram em redução da prevalência de FeLV nos Estados Unidos da América (EUA) e Europa, entretanto, no Brasil, determinadas regiões podem apresentar prevalências superiores a 50%. Esse estudo teve como objetivo avaliar a prevalência das retroviroses felinas, pelo teste de ensaio imunoenzimático, na região da Grande Vitória, no Espírito Santo, apontando ainda, a frequência de neoplasias nesses gatos. Um total de 388 gatos foram retrospectivamente avaliados (2014-2016). A prevalência de FIV foi de 2,3% e de FeLV 33,7%. Neoplasias foram identificadas nos três gatos sororeagentes para FIV e FeLV e em três gatos infectados apenas por FIV. Constatou-se a presença de neoplasias ainda em 26,6% dos gatos sororeagentes apenas para FeLV, com destaque para o linfoma na forma mediastinal. A elevada prevalência de FeLV demonstrada neste estudo aponta sobre a necessidade da instituição de medidas efetivas de controle, com destaque para a vacinação.

Effects of aquatic physiotherapy or health education program in women with fibromyalgia: a randomized clinical trial.

Background: Different treatments have been proposed for Fibromyalgia, but only few studies have compared their effects on multiples outcomes over time. Objective: The objective of this study was to investigate the effects of aquatic physiotherapy (AP) or a health education program (HEP) in a sample of women with Fibromyalgia (FM). Methods: Forty-six women with FM, aged between 25 and 60 years old, whose BMI was less than 30, were assigned to either AP (27 women) or HEP (19 women) groups in a blind randomized clinical trial lasting eleven weeks. Pain (McGill Pain questionnaire), fatigue (Piper Fatigue Scale-Revised), functional capability (Fibromyalgia Impact questionnaire), anxiety (Beck Anxiety Inventory), depression (Beck Depression Inventory) and quality of sleep (Pittsburgh Sleep Quality Index) data were collected at baseline, after six weeks and post intervention. Two-factor mixed-model analysis of variance (ANOVAs) were used to examine the effects of the treatment on each outcome variable. Results: The AP and HEP interventions showed statistically significant within-group differences on all outcome measures except reducing the pain. Between-group differences was statistically significant only for impact of FM on the participant's life [F(1.82,80.41) = 31,99; p ≤ 0.01] indicating that patients receiving HEP experienced a greater decrease in FIQ than those treated with AP. Conclusion: The findings do not allow to affirm that one intervention is superior to the other for the treatment of people with FM. Future studies should investigate whether the combination of HEP and PA can be effective and long-lasting.

Contradições entre a grande demanda e a pequena oferta do curso técnico em hemoterapia no brasil / Contradictions between the great demand and the small offer of the hemotherapy technician course in brazil / Contradiciones entre la gran demanda y la pequeña oferta del curso de técnico de hemoterapia en brasil

Resumo As regulamentações do setor sangue no Brasil passaram a exigir níveis de formação específicos voltados para os conhecimentos técnicos e práticos de hemoterapia e imunohematologia. O presente artigo objetivou identificar, compreender e analisar as imagens, ideias e percepções acerca do curso técnico em hemoterapia ministrado em uma escola de saúde pública da Região Sudeste do país. Metodologicamente, tratou-se de uma pesquisa-ação realizada entre março de 2012 e dezembro de 2013, com docentes, discentes e referências técnicas do curso. Constatamos que o curso foi considerado relevante, com bom conteúdo programático, apesar de sua centralização em uma capital. Concluímos que formação em hemoterapia se faz urgente, entretanto a inserção de técnicas no âmbito do processo e das relações do trabalho neste campo é marcada por fragilidades e ausência de diretrizes.

Abstract The blood sector regulations in Brazil started to require specific levels of schooling geared to technical and practical knowledge of hemotherapy and immunohematology. This article aimed to identify, understand and analyze the images, ideas and perceptions about hematology y technical course taught in a school of public health, in Brazil. Methodologically, this was action research carried out between March 2012 and December 2013, with teachers, students and technical references. We found that the course was considered relevant, with good content, despite its centralization in the city in the southeast of the country. We conclude that schooling in hematology is done urgently, however, inserting techniques and process of labor relations in the field of Hematology is marked by weakness and absence of the guidelines.

Resumen Las regulaciones del sector sangre en Brasil pasaron a exigir niveles de formación específicos dirigidos a los conocimientos técnicos y prácticos de hemoterapia e inmunohematología. El presente artículo objetivó identificar, comprender y analizar las imágenes, ideas y percepciones acerca del curso técnico de hemoterapia impartido en una escuela de salud pública de la Región Sudeste del País. Metodológicamente, se trató de una investigación-acción realizada entre marzo de 2012 y diciembre de 2013, con docentes, discentes y referencias técnicas del curso. Constatamos que el curso fue considerado relevante, con buen contenido programático, a pesar de su centralización en una capital. Concluimos que el entrenamiento en hemoterapia es urgente, al mismo tiempo que inserta técnicas no relacionadas al proceso ya las relaciones de trabajo en ese campo y marcadas por debilidades y ausencia de directrices.

Deforestation and malaria incidence in the legal Amazon Region between 1996 and 2012 / Desflorestamento e incidência de malária na região da Amazônia Legal entre 1996 e 2012

Abstract Malaria is a major public health problem, and Brazil is the largest contributor in America to the number of cases. In the country, the Amazon concentrates around 99.5% of the cases. This article aims to compare trends in deforestation rate and the parasite index of malaria in the Amazon region between 1996 and 2012. The annual crude rate of deforestation and the Annual Parasite Index (API) of malaria between 1996 and 2012 were estimated. The rate of deforestation has great variability among the states every year, and a similar situation is observed for malaria API over the studied period. There are important variations across the states within this period. The historical series of crude deforestation rate and malaria API have markedly similar trends; with a 1-2 years lag between them, suggesting that the occurrence of fluctuations in deforestation numbers impacts malaria numbers in a period immediately following. The mapping of deforested areas and the analysis of temporal series are therefore useful in the analysis of malaria.

Resumo A malária é um problema de saúde pública, sendo o Brasil, na América, o país que mais contribui com o número de casos. A Amazônia Legal concentra a maioria dos casos, cerca de 99,5%. O objetivo deste artigo é comparar as tendências da taxa de desflorestamento e o índice parasitário por malária na região da Amazônia Legal entre 1996 e 2012. Foram estimados a taxa bruta anual de desflorestamento e o Índice Parasitário Anual de Malária entre 1996 e 2012, para a Amazômia Legal e seus estados. Obtiveram-se a série histórica dos dois indicadores e as estatísticas descritivas por ano e por estado. A taxa de desflorestamento apresenta grande variabilidade entre os estados, ano a ano, e situação semelhante é observada para o IPA de malária no período. Há variação importante quando são observados os estados dentro do mesmo período. As séries históricas da taxa bruta de desflorestamento e do IPA de malária apresentam tendências semelhantes, com um lag de 1-2 anos entre elas, sugerindo que as oscilações nos números de desflorestamento impactam os números de malária em período imediatamente posterior. O mapeamento das áreas desflorestadas e a análise de séries temporais, portanto, são úteis na análise da malária.

High-risk alcohol use and anxiety and depression symptoms in adolescents and adults with type 1 diabetes mellitus: a cross-sectional study.

BACKGROUND: The medical literature shows that alcohol consumption is common among diabetic individuals and is associated with poor adherence to treatment, resulting in increased morbidity and mortality. However, no study has assessed the association between high-risk alcohol consumption and the presence of anxiety and depression in individuals with type 1 diabetes mellitus (1DM). METHODS: The present cross-sectional study was conducted in Uberlândia, Brazil, and it assessed 209 outpatients in relation to alcohol consumption and the presence of anxiety and depression symptoms, using the Alcohol Use Disorders Identification Test (AUDIT), the Hospital Anxiety and Depression (HAD) scale, and glycemic control, according to the levels of glycated hemoglobin (HbA1c). The chi-square test and logistic regression analysis were used to investigate the association between the investigated variables. RESULTS: The prevalence of high-risk alcohol consumption (AUDIT ≥ 8) among individuals with 1DM was high, specifically 24.9% among the entire group of subjects, 12.9% among the adolescents, 14.7% among the females, and 34.6% among the males. In comparisons based on gender and age, the odds of high-risk drinking were higher among males and participants aged 30 to 40 years (33.9%). The frequency of high-risk alcohol consumption did not differ as a function of gender among adolescents (females: 9.0%, males: 16.2%; p = 0.374). There was a linear trend in proportions related to the scores of anxiety and depression symptoms with high-risk alcohol consumption scores, indicating the association of these variables (p = 0.0229 and p = 0.0262, respectively). Moreover, the odds of female subjects exhibiting anxiety and depression symptoms were higher (odds ratio - OR: 4.4 and OR: 7.4, respectively). Glycemic control did not exhibit an association between high-risk alcohol consumption and the presence of anxiety and depression symptoms. CONCLUSIONS: The frequency of high-risk drinking increased along with age and was greater among males; however, this frequency did not exhibit differences in terms of gender among adolescents. There was a positive association between high risk alcohol consumption and anxiety and depression symptoms, although glycemic control was inadequate in most of the sample independent of alcohol consumption and the presence of anxiety and depression symptoms.

Cytotoxicity of phenothiazine derivatives associated with mitochondrial dysfunction: a structure-activity investigation.

Phenothiazine derivatives are neuroleptic drugs used in the treatment of schizophrenia and anxiety. Several side effects are described for these drugs, including hepatotoxicity, which may be related to their cytotoxic activity. Working with isolated rat liver mitochondria, we previously showed that phenothiazine derivatives induced the mitochondrial permeability transition associated with cytochrome c release. Since the mitochondrial permeabilization process plays a central role in cell death, the aim of this work was to evaluate the effects of five phenothiazine derivatives (chlorpromazine, fluphenazine, thioridazine, trifluoperazine, and triflupromazine) on the viability of hepatoma tissue culture (HTC) cells to establish the structural requirements for cytotoxicity. All phenothiazine derivatives decreased the viability of the HTC cells in a concentration-dependent manner and exhibited different cytotoxic potencies. The EC50 values ranged from 45 to 125 µM, with the piperidinic derivative thioridazine displaying the most cytotoxicity, followed by the piperazinic and aliphatic derivatives. The addition of the phenothiazine derivatives to cell suspensions resulted in significant morphological changes and plasma membrane permeabilization. Octanol/water partition studies revealed that these drugs partitioned preferentially to the apolar phase, even at low pH values (≤4.5). Also, structural and electronic properties were calculated employing density functional theory. Interestingly, the phenothiazine derivatives promoted an immediate dissipation of the mitochondrial transmembrane potential in HTC cells, and the EC50 values were closely correlated with those obtained in cell viability assays, as well as the EC50 for swelling in isolated mitochondria. These results significantly contribute to improving our understanding of the specific structural requirements of the phenothiazine derivatives to induce cell death and suggest the involvement of the mitochondrial permeability transition in phenothiazine-induced cytotoxicity in HTC cells.

Anxiety, depression, and satisfaction in close relatives of patients in an open visiting policy intensive care unit in Brazil.

PURPOSE: The purpose of the study is to evaluate the satisfaction and symptoms of anxiety and depression in family members in an open visiting intensive care unit (ICU). MATERIAL AND METHODS: A prospective study conducted in a 22-bed mixed ICU in a tertiary hospital in Sao Paulo, Brazil. Family members were enrolled 2 days after admission, and they completed a modified version of Critical Care Family Needs Inventory and the Hospital Anxiety and Depression Scale (HADS). RESULTS: A total of 471 families were interviewed between March 2011 and 2013. Prevalence of anxiety and depression were of 34% and 17%, respectively. We found a high satisfaction score, median of 13 (12-14), and 5% of families were dissatisfied. The daily visiting time was 12 (8-22) hours, and until 10 hours per day, there was a positive association between visiting hours and satisfaction (P = .004). Dissatisfied families reported higher rates of anxiety and depression (P < .001). Visiting hours presented negative interaction between HADS and Critical Care Family Needs Inventory. Patients' severity had a trend toward dissatisfaction (P = .08) and affected emotional disorders (P < .001). CONCLUSIONS: Family members in an open visit ICU reported low rates of symptoms of anxiety and depression and high satisfaction. Visiting hours were associated with the satisfaction score and were a protective factor for family members with higher HADS score.

Oxidative Stress in Maternal Blood and Placenta From Mild Diabetic Rats.

The aim of the present study was at evaluating the effects of oxidative stress in blood and placenta of mild diabetic Wistar rats. At birth, Wistar rats received citrate buffer (nondiabetic group, n = 15) and another group received streptozotocin (100 mg/kg, subcutaneous) to induce mild diabetes (diabetic, n = 15). The glycemia of these pregnant adult female rats were evaluated at days 0, 7, 14, and 21 of pregnancy, and at term pregnancy, the blood and placental samples were collected for oxidative stress measurements. The mild diabetes caused glycemia superior to 120 mg/dL during pregnancy, increased superoxide dismutase, glutathione peroxidase, glutathione reductase activities, and malondialdehyde levels in the blood, and catalase activity in the placenta. Thus, mild diabetes increased activities of antioxidant substances aiming at defending against the exacerbated oxidative stress but were not enough. The placenta also answered to diabetic milieu and increased antioxidant defense, showing that even a mild hyperglycemia was enough to cause placental and maternal blood changes.

Monitoramento telefônico como estrategia educativa para o autocuidado das pessoas com diabetes na atenção primária / Montoreo telefónico como estrategia educativa para el autocuidado de personas con diabetes en atención primaria / Telephone-based monitoring: an educational strategy for diabetes self-care at the primary health care level

Objetivos: Descrever a experiência do monitoramento telefônico como estratégia educativa na educação do autocuidado da pessoa com diabetes na atenção primária. Material e método: Trata-se de um estudo descritivo, realizado com 115 usuários com diabetes residentes em domicílios com linha telefônica fxa e em seguimen-to no programa educativo na atenção primária, ano de 2010. As entrevistas telefônicas seguiram um roteiro semiestruturado. Resultados: Dos 115 entrevistados, 44,8 por cento praticam atividade física e 58,3 por cento seguem a dieta. Em ambos os grupos, a maioria dos praticantes de atividade física são adeptos da caminhada. O percentual de usuários que seguem a dieta é praticamente o mesmo entre homens e mulheres (60,0 e 57,9 por cento). Conclusão: O monitoramento permitiu reforçar a comunicação e as informações para a educação dos usuários com diabetes, fortaleceu o vínculo entre usuários e profissionais e possibilitou que os profissionais interviessem junto às dif-culdades dos usuários no manejo da doença, de forma rápida e efetiva.

Objective: to describe the experience of telephone-based monitoring as an educational strategy to teach self-care to people with diabetes at the primary health care level. Material and method: This descriptive study was performed with 115 clients with diabetes who had a landline telephone at home and were being followed in a primary health care educational program in 2010. Te telephone interviews were performed following a semi-structured script. Results: From the 115 interviewed clients, 44.8 percent were physically active and 58.3 percent followed a diet. In both groups, most of those engaged in physical activity reported walking. Basically the same percentage was found for male and female clients who followed their diet (60.0 percent and 57.9 percent, respectively). Conclusion: Monitoring allowed reinforcing communication and the information required for the education of clients with diabetes, it strengthened the bond between clients and professionals, and permitted the professionals to intervene on the difficulties that the clients presented regarding their management of the diseases, in a fast and effective way.

Objetivo: Describir la experiencia del monitoreo telefónico como estrategia educativa en enseñanza del auto-cuidado de personas con diabetes en la atención primaria. Material y método: Estudio descriptivo, realizado con 115 pacientes de diabetes residentes en domicilios con línea telefónica fja y en seguimiento educativo en la atención primaria, año 2010. Entrevistas telefónicas fueron realizadas según rutina semiestructurada. Resultados: De los 115 entrevistados, 44,8 por ciento practican actividad física y 58,3 por ciento siguen la dieta. En ambos grupos, la mayoría de practicantes de actividad física son adeptos a las caminatas. El porcentaje de pacientes que sigue la dieta es casi el mismo entre hombres y mujeres (60,0 y 57,9 por ciento). Conclusión: el monitoreo permitió reforzar la comunicación y las informaciones para educar a los pacientes con diabetes, fortaleció el vínculo entre pacientes y profesionales y posibilitó que éstos intervinieran ante las dificultades de los pacientes en el manejo de la enfermedad, de forma rápida y efectiva.

Cytochrome c-promoted cardiolipin oxidation generates singlet molecular oxygen.

The interaction of cytochrome c (cyt c) with cardiolipin (CL) induces protein conformational changes that favor peroxidase activity. This process has been correlated with CL oxidation and the induction of cell death. Here we report evidence demonstrating the generation of singlet molecular oxygen [O(2)((1)Δ(g))] by a cyt c-CL complex in a model membrane containing CL. The formation of singlet oxygen was directly evidenced by luminescence measurements at 1270 nm and by chemical trapping experiments. Singlet oxygen generation required cyt c-CL binding and occurred at pH values higher than 6, consistent with lipid-protein interactions involving fully deprotonated CL species and positively charged residues in the protein. Moreover, singlet oxygen formation was specifically observed for tetralinoleoyl CL species and was not observed with monounsaturated and saturated CL species. Our results show that there are at least two mechanisms leading to singlet oxygen formation: one with fast kinetics involving the generation of singlet oxygen directly from CL hydroperoxide decomposition and the other involving CL oxidation. The contribution of the first mechanism was clearly evidenced by the detection of labeled singlet oxygen [(18)O(2)((1)Δ(g))] from liposomes supplemented with 18-oxygen-labeled CL hydroperoxides. However quantitative analysis showed that singlet oxygen yield from CL hydroperoxides was minor (<5%) and that most of the singlet oxygen is formed from the second mechanism. Based on these data and previous findings we propose a mechanism of singlet oxygen generation through reactions involving peroxyl radicals (Russell mechanism) and excited triplet carbonyl intermediates (energy transfer mechanism).

Diagnósticos de enfermagem em paciente submetido à terapia de reposição renal contínua / Nursing diagnoses in paqtient who received continuous renal replacement therapy

A insuficiência renal aguda é uma complicação freqüente em pacientes críticos, sendo a terapia de reposição renal continua o método de tratamento mais usado nesta população, com uma taxa de utilização de 80%. Este método exige do enfermeiro conhecimento e habilidades para o raciocínio clinico, sendo fundamental a aplicação dos diagnósticos de enfermagem para a definição de condutas. Objetivo: Identificar diagnósticos de enfermagem mais freqüentes em um paciente submetido à este tratamento. Método: Relato de caso de um paciente com diagnóstico de insuficiência renal aguda após internação na unidade de terapia intensiva e em uso de terapia de reposição renal contínua. Os diagnósticos de enfermagem foram elencados diariamente por meio de dados do prontuário durante os dias do tratamento. Resultados: Foram identificados 7 diagnósticos, sendo que destes, 6 obtiveram uma freqüência de 100% e apenas 1 obteve uma freqüência de 50%. Os diagnósticos identificados foram perfusão tissular renal ineficaz, volume de líquidos excessivo, proteção ineficaz, débito cardíaco diminuído, risco de integridade da pele prejudicada, risco para infecção e termorregulação ineficaz. Conclusão: Os diagnósticos encontrados coincidem com outros estudos de pacientes em tratamento de diálise intermitente e estão relacionados aos eventos desencadeados por este tratamento.

Specific effects of reactive thiol drugs on mitochondrial bioenergetics.

In this minireview, the more recent findings about the effects of peculiar reactive thiol drugs on mitochondria are presented. These include the following compounds: metallo meso-tetrakis porphyrins, palladacycles, telluranes and phenothiazines. Metallo meso-tetrakis porphyrins can exhibit both beneficial and deleterious effects on mitochodria that are modulated by the central metal, cell location, and availability of axial ligands. Therefore, these compounds have the versatility to be used for cell and mitochondria protection and death. The antioxidant activity of manganese porphyrins is related to a glutathione peroxidase-like activity. By attacking exclusively the membrane protein thiol groups without glutathione depletion, palladacycles are able to induce mitochondrial permeability transition (MPT) and cytochrome c release in the absence of oxidative stress. In hepatoma cells, the mitochondrial action of palladacycles was able to induce apoptotic death. As opposed to palladacycles, telluranes and phenothiazines are able to conjugate the capacity to promote the MPT in a dose-dependent manner in association with efficient antioxidant activity toward lipids. These studies demonstrated that the action of drugs on mitochondrial bioenergetics can be modulated by peculiar reactivity with thiol groups. Therefore, they contribute to studies of toxicity as well as the design of new drugs.

Systematic review of lymphoma in oral cavity and maxillofacial region.

The aim of this paper is to determine, compare and summarize presentation characteristics of patients with lymphoma of the oral cavity and maxillofacial region using the results of different primary studies. Articles from the PubMed database published between 1990 and 2010 were reviewed. The key words "Lymphoma" and "Oral" were used to search for titles, identifying 215 articles, of which 178 were excluded and 37 were used for this review. After evaluation, the researchers agreed to include 15 of the papers in the study. This paper describes the author year published, number of cases, sex, age, location, signs and symptoms, classification, diagnosis, staging and treatment reported in each of the 15 studies. A total 714 patients are described. In 11 out of 13 studies, there were more cases in males than females. Ages ranged from 3 to 96 years. Presentation was most frequently the gingiva and the main sign was swelling. Staging was described for only 177 cases and treatment was described for only 110 cases. It is concluded that the presentation of most of the lymphomas of the oral cavity and maxillofacial region is extranodal, non-Hodgkin's. The most common locations are gingiva in the oral activity and Waldeyers's ring in the maxillofacial region. Although these lymphomas are rare pathological entities, it is important to describe the complete manifestation of their natural history in order to provide knowledge of their development.

On the mechanisms of phenothiazine-induced mitochondrial permeability transition: Thiol oxidation, strict Ca2+ dependence, and cyt c release.

Phenothiazines (PTZ) are drugs widely used in the treatment of schizophrenia. Trifluoperazine, a piperazinic PTZ derivative, has been described as inhibitor of the mitochondrial permeability transition (MPT). We reported previously the antioxidant activity of thioridazine at relatively low concentrations associated to the inhibition of the MPT (Brit. J. Pharmacol., 2002;136:136-142). In this study, it was investigated the induction of MPT by PTZ derivatives at concentrations higher than 10 microM focusing on the molecular mechanism involved. PTZ promoted a dose-response mitochondrial swelling accompanied by mitochondrial transmembrane potential dissipation and calcium release, being thioridazine the most potent derivative. PTZ-induced MPT was partially inhibited by CsA or Mg(2+) and completely abolished by the abstraction of calcium. The oxidation of reduced thiol group of mitochondrial membrane proteins by PTZ was upstream the PTP opening and it was not sufficient to promote the opening of PTP that only occurred when calcium was present in the mitochondrial matrix. EPR experiments using DMPO as spin trapping excluded the participation of reactive oxygen species on the PTZ-induced MPT. Since PTZ give rise to cation radicals chemically by the action of peroxidases and cyanide inhibited the PTZ-induced swelling, we propose that PTZ bury in the inner mitochondrial membrane and the chemically generated PTZ cation radicals modify specific thiol groups that in the presence of Ca(2+) result in MPT associated to cytochrome c release. These findings contribute for the understanding of mechanisms of MPT induction and may have implications for the cell death induced by PTZ.

Superoxide radical protects liposome-contained cytochrome c against oxidative damage promoted by peroxynitrite and free radicals.

The effects of nitrosative species on cyt c structure and peroxidase activity were investigated here in the presence of O(2)(*-) and anionic and zwitterionic vesicles. Nitrosative species were generated by 3-morpholinesydnonymine (SIN1) decomposition, using cyt c heme iron and/or molecular oxygen as electron acceptor. Far- and near-UV CD spectra of SIN1-treated cyt c revealed respectively a slight decrease of alpha-helix content (from 39 to 34%) and changes in the tryptophan structure accompanied by increased fluorescence. The Soret CD spectra displayed a significant decrease of the positive signal at 403 nm. EPR spectra revealed the presence of a low-spin cyt c form (S=1/2) with g(1)=2.736, g(2)=2.465, and g(3)=2.058 after incubation with SIN1. These data suggest that the concomitant presence of NO(*) and O(2)(*-) generated from dissolved oxygen, in a system containing cyt c and liposomes, promotes chemical and conformational modifications in cyt c, resulting in a hypothetical bis-histidine hexacoordinated heme iron. We also show that, paradoxically, O(2)(*-) prevents not only membrane lipoperoxidation by peroxide-derived radicals but also oxidation of cyt c itself due to the ability of O(2)(*-) to reduce heme iron. Finally, lipoperoxidation measurements showed that, although it is a more efficient peroxidase, SIN1-treated cyt c is not more effective than native cyt c in promoting damage to anionic liposomes in the presence of tert-ButylOOH, probably due to loss of affinity with negatively charged lipids.

Palladacycles catalyse the oxidation of critical thiols of the mitochondrial membrane proteins and lead to mitochondrial permeabilization and cytochrome c release associated with apoptosis.

Permeabilization of the mitochondrial membrane has been extensively associated with necrotic and apoptotic cell death. Similarly to what had been previously observed for B16F10-Nex2 murine melanoma cells, PdC (palladacycle compounds) obtained from the reaction of dmpa (N,N-dimethyl-1-phenethylamine) with the dppe [1,2-ethanebis(diphenylphosphine)] were able to induce apoptosis in HTC (hepatoma, tissue culture) cells, presenting anticancer activity in vitro. To elucidate cell site-specific actions of dmpa:dppe that could respond to the induction of apoptosis in cancer cells in the present study, we investigated the effects of PdC on isolated RLM (rat liver mitochondria). Our results showed that these palladacycles are able to induce a Ca2+-independent mitochondrial swelling that was not inhibited by ADP, Mg2+ and antioxidants. However, the PdC-induced mitochondrial permeabilization was partially prevented by pre-incubation with CsA (cyclosporin A), NEM (N-ethylmaleimide) and bongkreic acid and totally prevented by DTT (dithiothreitol). A decrease in the content of reduced thiol groups of the mitochondrial membrane proteins was also observed, as well as the presence of membrane protein aggregates in SDS/PAGE without lipid and GSH oxidation. FTIR (Fourier-transform IR) analysis of PdC-treated RLM demonstrated the formation of disulfide bonds between critical thiols in mitochondrial membrane proteins. Associated with the mitochondrial permeabilization, PdC also induced the release of cytochrome c, which is sensitive to inhibition by DTT. Besides the contribution to clarify the pro-apoptotic mechanism of PdC, this study shows that the catalysis of specific protein thiol cross-linkage is enough to induce mitochondrial permeabilization and cytochrome c release.