ABSTRACT
AIMS: The aim of this study was to investigate the potential of aureocin A53, a staphylococcal antimicrobial peptide, for improving food safety. METHODS AND RESULTS: The antimicrobial activity of aureocin A53 against strains of Listeria monocytogenes isolated from food was tested and the bacteriocin proved to be bactericidal and bacteriolytic against the listerial strains. Aureocin A53 was neither toxic to eukaryotic cell lines nor haemolytic against sheep erythrocytes. It also exhibited a remarkable stability during storage at different temperatures and sensitivity to both simulated gastric juice and bile salts. When the antibacterial activity of aureocin A53 (256 AU ml(-1) ) was tested in skimmed milk artificially inoculated with a L. monocytogenes strain (1·0 × 10(4) CFU ml(-1) ) isolated from food, during storage at 4°C, the bacteriocin reduced the viable counts by 7·7-log10 units up to 7 days of incubation, when compared with the controls not treated with the bacteriocin. CONCLUSIONS: Aureocin A53 exhibited several features considered important for biopreservation and remained fully active in a food matrix. SIGNIFICANCE AND IMPACT OF THE STUDY: Taken together, the results confirmed that aureocin A53 has potential to be used as a food preservative, representing an alternative to the use of nisin in biopreservation of dairy products.
Subject(s)
Anti-Bacterial Agents/pharmacology , Food Preservation/methods , Food Preservatives/pharmacology , Milk/microbiology , Peptides/pharmacology , Animals , Antimicrobial Cationic Peptides , Food Preservation/instrumentation , Listeria monocytogenes/drug effects , Listeria monocytogenes/isolation & purification , SheepABSTRACT
Atherosclerosis is morphologically an inflammatory disease, where endothelial dysfunction plays a key role in all the stages. The nitric oxide (NO) synthase 3 (NOS3) gene is responsible for the synthesis of endothelial NO synthase (eNOS) in humans and some genetic polymorphisms are considered "polymorphisms associated with risk" for the development of coronary artery diseases, such as acute coronary syndrome. Thus, the present study aimed to evaluate the influence of the -786T>C polymorphism of the eNOS gene on inflammatory and oxidative process. A prospective cohort study of 125 consecutive patients with clinical diagnosis of non-ST-elevation acute coronary syndromes was conducted. Patients were assessed using a standardized questionnaire. Blood samples were drawn to measure serum levels of high-sensitivity C-reactive protein, soluble CD40 ligand, interleukin-6 (IL-6), N-terminal prohormone of brain natriuretic peptide, immunoglobulin G antibodies against oxidized low-density lipoprotein. The genotypes for the -786T>C polymorphism in the 5'-flanking region of eNOS gene were determined. The -786C allele was found in 92 of 250 alleles (38.8%). No statistical association was observed between demographic and clinical characteristics and distribution of eNOS-786T>C polymorphism. We found that -786CC was associated with lower levels of IL-6. No significant differences were observed between the distribution of -786T>C polymorphism and other investigated markers.
Subject(s)
Coronary Artery Disease/genetics , Interleukin-6/genetics , Nitric Oxide Synthase Type III/genetics , Polymorphism, Genetic , Aged , Base Sequence , DNA Primers , Female , Humans , Male , Middle Aged , Polymerase Chain ReactionABSTRACT
Psychotria is a complex genus whose neotropical species are known by the presence of glucosidic monoterpene indole alkaloids. These compounds are able to display a large range of effects on the central nervous system, such as anxiolytic, antidepressant, analgesic, and impairment of learning and memory acquisition. The aims of this study were to investigate the effects displayed by strictosidinic acid, isolated from Psychotria myriantha Mull. Arg. (Rubiaceae) leaves, on monoamine levels in rat hippocampus and on monoamine oxidase activity. A significance (p<0.01) of 83.5% reduction in 5-HT levels was observed after intra-hippocampal injection (20 µg/µl). After treatment by intraperitoneal route (10 mg/kg), a 63.4% reduction in 5-HT levels and a 67.4% reduction in DOPAC values were observed. The results indicate that strictosidinic acid seems to act on 5-HT system in rat hippocampus, possibly inhibiting precursor enzymes of 5-HT biosynthesis. The decrease verified in DOPAC levels suggests a role of strictosidinic acid in the dopaminergic transmission, probably due to an inhibition of monoamine oxidase activity, confirmed by the enzymatic assay, which demonstrated an inhibitory effect on MAO A in rat brain mitochondria.
Subject(s)
3,4-Dihydroxyphenylacetic Acid/metabolism , Carbolines/pharmacology , Glycosides/pharmacology , Hippocampus/metabolism , Monoamine Oxidase/metabolism , Plant Extracts/pharmacology , Psychotria/chemistry , Serotonin/metabolism , Animals , Carbolines/administration & dosage , Carbolines/isolation & purification , Glycosides/administration & dosage , Glycosides/isolation & purification , Male , Mitochondria/drug effects , Mitochondria/metabolism , Monoamine Oxidase Inhibitors/administration & dosage , Monoamine Oxidase Inhibitors/isolation & purification , Monoamine Oxidase Inhibitors/pharmacology , Plant Extracts/administration & dosage , Plant Extracts/chemistry , Plant Leaves , Rats , Rats, Wistar , Serotonin/biosynthesis , Serotonin Antagonists/administration & dosage , Serotonin Antagonists/isolation & purification , Serotonin Antagonists/pharmacologyABSTRACT
Strictosidinic acid 10mg/kg, isolated from Psychotria myriantha leaves, were administered intraperitoneally to Wistar male rats (n=5-6). After 60 minutes, striatum was dissected, homogenized and injected onto HPLC-ED chromatographic system. It was observed a 28.7% reduction in the 5-HT levels followed up by an increase of 5-HIAA levels (29.4%). Furthermore there was a decrease of 8.0% in DA levels and an increase in the levels of metabolites DOPAC (21.5%) and HVA (52.5%). The results indicate that strictosidinic acid has a promising effect in the central nervous system, justifying more studies about the central actions of Psychotria compounds.
Subject(s)
Carbolines/pharmacology , Corpus Striatum/drug effects , Dopamine/metabolism , Glycosides/pharmacology , Neurotransmitter Agents/pharmacology , Plant Extracts/pharmacology , Rubiaceae/chemistry , Serotonin/metabolism , 3,4-Dihydroxyphenylacetic Acid/metabolism , Animals , Carbolines/isolation & purification , Corpus Striatum/metabolism , Glycosides/isolation & purification , Homovanillic Acid/metabolism , Injections, Intraperitoneal , Male , Neurotransmitter Agents/administration & dosage , Neurotransmitter Agents/isolation & purification , Phenylacetates/metabolism , Plant Extracts/administration & dosage , Plant Extracts/chemistry , Plant Leaves , Rats , Rats, Wistar , Serotonin/analogs & derivativesABSTRACT
Mikania laevigata Schultz Bip. ex Baker, M. involucrata Hook. et Arn. and M. hirsutissima DC. (Asteraceae), commonly occurring in the southern Brazilian State of Rio Grande do Sul, were submitted to biological tests to evaluate their potential antiinflammatory activity. Decoctions from the leaves and stems were analysed by the induced rat paw oedema and pleurisy models. The animals were treated orally with different decoction doses. In the induced rat paw oedema test, the animals treated with leaf decoctions from M. laevigata (200 mg/ kg) and M. involucrata (50 mg/ kg) presented an oedema inhibition of 81.56% and 81.67%, respectively, 3 h after the administration of the phlogistic agent. Leaf decoctions from M. hirsutissima (400 mg/ kg) did not show such an activity. Stem decoctions displayed lower antiinflammatory activity when compared with the same doses and response time of the leaf decoctions for all analysed species. In the pleurisy assay, leaf decoctions from M. laevigata (400 mg/ kg) and M. involucrata (200 mg/ kg) inhibited leukocyte migration to the pleural exudate by 28.26% and 54.35%, respectively.
