ABSTRACT
BACKGROUND: Many studies are in the literature on the ANP and oxytocin-presence in the brain, but very few studies with controversial results are reported on the presence of these peptides in the cerebellum. This immunohistochemical study investigates on the ANP and oxytocin-presence in the cerebellum of the adult rat and mouse rodents. RESULTS: This study, firstly, evidences the ANP- immunopositivity in cerebellar cortex of both rat and mouse rodents. In rat the molecular layer presents some few immunopositive fibers, but no neuron resulted immunopositive; the granular and Purkinje cells are immunopositive. In mouse the cerebellar cortex ANP-immunopositivity is present in all layers. The oxytocin-presence in the rat the afferent fibers are immunopositive are in the granular layer; in mouse the OT-immunopositivity is in the molecular layer only. CONCLUSIONS: This study, firstly, shows that ANP and OT are present in the cerebellar cortex both in rat and mouse rodents. In the mouse cerebellar cortex ANP-presence is more diffuse and OT- localization differences in the two species.
ABSTRACT
Our immunohistochemical study shows that atrial natriuretic peptide (ANP) is present in both human fetal and adult parotid gland. In the fetuses ANP is localized in cell clusters, while in adult parotid gland, the ANP is seen only in the wall of intra- and extralobular ducts. The results suggest that ANP might play a role in the differentiation of the parotid gland similarly as in brain and that during fetal growth ANP may play a role in the regulating the secretion of primitive salivary fluid, even if acini are still not developed.
Subject(s)
Atrial Natriuretic Factor/metabolism , Fetus/embryology , Fetus/metabolism , Gestational Age , Parotid Gland/metabolism , Adult , Fetus/cytology , Humans , Male , Parotid Gland/cytologyABSTRACT
Atrial natriuretic peptide was found in the heart of vertebrates, we studied the ANP presence in the heart of Ciona intestinalis. This is animal is very important because of the its evolutionary position between invertebrates and vertebrates. ANP presence was only revealed in myoepithelial cells of the myocardium. Results suggest the hypothesis that ANP is present not only in the vertebrates but also in the invertebrates and in Ciona heart ANP might play a similar role like in the heart of vertebrates.
Subject(s)
Atrial Natriuretic Factor/metabolism , Ciona intestinalis/metabolism , Myocardium/metabolism , Animals , Ciona intestinalis/cytology , Myocardium/cytologyABSTRACT
Aim of this work is to investigate the presence of Atrial natriuretic peptide (ANP) and insulin in human foetal pancreas from not diabetic and diabetic mothers. The data literature evidenced relationship between serum ANP and insulin. The our work carried out on sections immunostained for ANP and insulin showed positivity for ANP and insulin in beta-cells of pancreas from diabetic mothers, while positivity for insulin in beta-cells of foetuses from not diabetic mothers. Results indicate that the insulin presence stimulates the ANP synthesis in pancreas of human foetuses from diabetic mothers.
