ABSTRACT
Pineapple litter has a complex polymer of cellulose, hemicellulose, and lignin, which makes them difficult to decompose. However, pineapple litter has great potential to be a good organic material source for the soil when completely decomposed. The addition of inoculants can facilitate the composting process. This study investigated whether the addition of cellulolytic fungi inoculants to pineapple litters improves the efficiency of the composting processes. The treatments were KP1 = pineapple leaf litter: cow manure (2:1), KP2 = pineapple stem litter: cow manure (2:1), KP3 = pineapple leaf litter: pineapple stem litter: cow manure P1 (leaf litter and 1% inoculum), P2 (stem litter and 1% inoculum), and P3 (leaf + stem litters and 1% inoculum). The result showed that the number of Aspergillus sp. spores on corn media was 5.64 x 107 spores/mL, with viability of 98.58%. Aspergillus sp. inoculum improved the quality of pineapple litter compost, based on the enhanced contents of C, N, P, K, and the C/N ratio, during the seven weeks of composting. Moreover, the best treatment observed in this study was P1. The C/N ratios of compost at P1, P2, and P3 were within the recommended range of organic fertilizer which was 15-25%, with a Carbon/Nitrogen proportion of 11.3%, 11.8%, and 12.4% (P1, P2, and P3), respectively.
ABSTRACT
Pineapple plantations leave a lot of plant biomass after the planting season ends. The abundant residue of pineapple plants causes problems due to the high content of lignocellulose, which is difficult to decompose naturally. This study aimed to isolate and characterize lignocellulolytic microfungi isolates from pineapple plantations. The information of this study was used as data to prepare an inoculum for the induction of pineapple litter composting that was resistant to stress to pineapple plantation habitat. The results showed that there were 11 dominant lignocellulolytic microfungi isolates found from pineapple litter and plantation soil. Using selective media, the selection showed five cellulolytic (Bioggp 3, 6, 9, 11, and 12); five xylanolytic (Bioggp 3, 6, 8, 9, and 12); and two ligninolytic microfungi isolates (Bioggp 2 and 5). Bioggp 3, 6, 9, and 12 are cellulolytic and xylanolytic with Bioggp 3 showing the highest cellulolytic index (4.0) and xylanolytic index (4.20). Testing of ligninolytic microfungi showed that the Bioggp 5 isolate had a stronger lignin indicator (color intensity = 4.0 and zone ratio of 1.47) than the Bioggp 2 isolate. Bioggp 9 had the highest cellulolytic isolate spore productivity at 4.5 × 108 spores/mL with 93.3% spore viability, and Bioggp 3 had the highest xylanolytic isolate spore productivity at 2.5 × 109 spores/mL with 89.3% spore viability. Bioggp 2 had the highest ligninolytic isolate spore productivity at 1.8 × 109 spores/mL, but Bioggp 5 showed the highest spore viability at 98.0%.
