ABSTRACT
OBJECTIVE: Endometriosis is a common and painful condition characterised by the formation of endometrial lesions within the peritoneal cavity. Previous studies have suggested a role for hedgehog signalling in the pathogenesis of endometriosis. We investigated the role of hedgehog signalling in the establishment of endometriosis lesions using 5E1, a hedgehog ligand neutralising antibody, and a mouse model of endometriosis. To mimic the initiation of endometriosis by retrograde menstruation, which is believed to occur in humans, donor mice underwent an artificial menstruation protocol. Fragments of menstrual endometrium were injected into the peritoneal cavity of estrogen primed recipients. Recipients received twice weekly injections of 5E1 or an isotype matched control antibody for three weeks. Lesions were collected and analysed for markers of epithelium, proliferation and apoptosis by immunofluorescence microscopy. RESULTS: Treatment with 5E1 reduced the number of lesions found on the mesentery. No significant changes were found in the size of lesions, abundance of endometrial epithelial cells, proliferation or apoptosis.
Subject(s)
Endometriosis , Hedgehog Proteins , Animals , Antibodies, Neutralizing , Endometriosis/drug therapy , Endometrium , Female , Humans , Ligands , Mice , Signal TransductionABSTRACT
The letter to Florence Nightingale was written by Bernita Decker as part of a nursing course assignment for our Nurse Educator advisor, Betty Pugh. Florence Nightingale's response to the student was written by Joanne Farley, a Nightingale historian, who recently returned from a trip to England and Ireland to study original Nightingale documents and historic sites.
Subject(s)
Education, Nursing/standards , Gender Identity , Job Description , Nursing Care/methods , HumansABSTRACT
As more graduate programs emphasize the preparation of expert clinicians, fewer masters-prepared nurses have been offered the opportunity to select a teaching track. As a result, new faculty members in colleges and universities have limited knowledge and skills in basic education concepts. The articles in this series will address some of these basic concepts, providing practical information for new faculty members in the areas of test development, use of audio-visuals, classroom methods, and teaching strategies.
Subject(s)
Audiovisual Aids , Education, Nursing , Teaching/methods , HumansSubject(s)
Education, Nursing , Teaching/methods , Group Processes , Humans , Learning , Students, Nursing/psychologySubject(s)
Military Nursing/history , England , Hemorrhagic Fever, Crimean/history , History, 19th Century , HumansABSTRACT
As more graduate programs emphasize the preparation of expert clinicians, fewer masters-prepared nurses have been offered the opportunity to select a teaching track. As a result, new faculty members in colleges and universities have limited knowledge and skills in basic education concepts. The articles in this series will address some of these basic concepts, providing practical information for new faculty members in the areas of test development, use of audio-visuals, classroom methods, and teaching strategies.
