Effects Of Metformin On The Weight Of Healthy And Streptozotocin-Induced Diabetic Animal Model.

BACKGROUND: Metformin was approved by FDA in 1994 for the treatment of type II diabetes mellitus. Due to its interaction with the AMP kinases enzyme system, it showed multiple effects on other organ systems of the body. Despite being the drug of the first choice for type II diabetes it became a popular drug for PCOS and currently, various trials are proving its anticancer effects. Though not approved by FDA it is also responsible for weight loss. The study aims to investigate the effect of metformin on the weight of healthy and streptozotocin-induced diabetic animal models. The study was performed at Animal House, Faculty of Pharmacy Ziauddin University Karachi. METHODS: It was a pre-clinical experimental study conducted at Ziauddin University Karachi from August 2019 to December 2019. 36 male Albino rats were divided into 6 groups such with 6 rats in each group. Out of six three were healthy models (i.e., groups 1, 2, and 3) and the remaining three were diabetic models (i.e., Group 4, 5, and 6). Diabetes was induced in groups 4,5 and 6 by using the diabetogenic drug Streptozotocin. In Group 1 and 4, 0.5 ml normal saline was administered, 50 mg/kg (5 mg/100g) metformin diluted in 0.5 ml distilled water was administered in Group 2 and 5 followed by administration of 80 mg/kg (8 mg/100g) metformin diluted in 0.5ml distilled water in Group 3 and 6. the protocol was followed twice a day for 42 days. The weight of all the groups was calculated before and after drug intervention. RESULTS: Metformin-induced weight loss was observed in healthy treated groups at both doses, however, there was no significant difference in weight loss in diabetic treated and untreated groups. CONCLUSIONS: Metformin showed weight lowering properties in healthy treated subjects, after induction of diabetes we found weight loss in diabetic untreated and treated groups which suggests that hyperglycaemia may cause weight loss but when metformin corrects the hyperglycaemic profile it does not lead to an increase in weight.

Frequency and antibiogram of multi-drug resistant pseudomonas aeruginosa in a Tertiary Care Hospital of Pakistan.

OBJECTIVE: To determine pathogen burden and susceptibility pattern of multi-drug resistant (MDR) Pseudomonas aeruginosa isolates from clinical specimens in Karachi. METHODS: It was In-vitro Clinical study, conducted in department of Pharmacology, Ziauddin University, and isolates were collected from various specimens such as pus, tracheal aspiration, wound swab, blood and urine in Microbiology department of Ziauddin Hospital, Nazimabad campus, Karachi. The antibiotic susceptibility pattern was determined by Kirby Bauer Disc diffusion method. Samples were processed as per procedures defined by Clinical and Laboratory Standards Institute (CLSI) guidelines 2018. RESULTS: About 55% were found to be multi drug resistant P. aeruginosa. Majority of the isolates (35.4%) were recovered from the age range 60-80 years. Maximum number of MDR P. aeruginosa was isolated from pus (33.1%) followed by tracheal aspiration (20.6%). Highest sensitivity was seen by colistin (100%) followed by ceftolozane/tazobactam (60%). Least sensitivity was observed with imipenem (19%). However, increase trend of resistance was seen among all antipesudomonal drugs. CONCLUSION: Increasing frequency of infections due to MDR P. aeruginosa is an emerging threat in our set up which can be prevented by prescribing antibiotics judiciously. Consistent lab detection and surveillance regarding this resistant pathogen is compulsory for providing effective health care to community.