The N400 for brain computer interfacing: complexities and opportunities.

The N400 is an event related potential that is evoked in response to conceptually meaningful stimuli. It is for instance more negative in response to incongruent than congruent words in a sentence, and more negative for unrelated than related words following a prime word. This sensitivity to semantic content of a stimulus in relation to the mental context of an individual makes it a signal of interest for Brain Computer Interfaces. A complicating aspect is the number of factors that can affect the N400 amplitude. In this paper, we provide an accessible overview of this range of N400 effects, and survey the three main BCI application areas that currently exploit the N400: (1) exploiting the semantic processing of faces to enhance matrix speller performance, (2) detecting language processing in patients with Disorders of Consciousness, and (3) using semantic stimuli to probe what is on a user's mind. Drawing on studies from these application areas, we illustrate that the N400 can successfully be exploited for BCI purposes, but that the signal-to-noise ratio is a limiting factor, with signal strength also varying strongly across subjects. Furthermore, we put findings in context of the general N400 literature, noting open questions and identifying opportunities for further research.

Sequenced subjective accents for brain-computer interfaces.

Subjective accenting is a cognitive process in which identical auditory pulses at an isochronous rate turn into the percept of an accenting pattern. This process can be voluntarily controlled, making it a candidate for communication from human user to machine in a brain-computer interface (BCI) system. In this study we investigated whether subjective accenting is a feasible paradigm for BCI and how its time-structured nature can be exploited for optimal decoding from non-invasive EEG data. Ten subjects perceived and imagined different metric patterns (two-, three- and four-beat) superimposed on a steady metronome. With an offline classification paradigm, we classified imagined accented from non-accented beats on a single trial (0.5 s) level with an average accuracy of 60.4% over all subjects. We show that decoding of imagined accents is also possible with a classifier trained on perception data. Cyclic patterns of accents and non-accents were successfully decoded with a sequence classification algorithm. Classification performances were compared by means of bit rate. Performance in the best scenario translates into an average bit rate of 4.4 bits min(-1) over subjects, which makes subjective accenting a promising paradigm for an online auditory BCI.

Shared mechanisms in perception and imagery of auditory accents.

OBJECTIVE: An auditory rhythm can be perceived as a sequence of accented (loud) and non-accented (soft) beats or it can be imagined. Subjective rhythmization refers to the induction of accenting patterns during the presentation of identical auditory pulses at an isochronous rate. It can be an automatic process, but it can also be voluntarily controlled. We investigated whether imagined accents can be decoded from brain signals on a single-trial basis, and if there is information shared between perception and imagery in the contrast of accents and non-accents. METHODS: Ten subjects perceived and imagined three different metric patterns (two-, three-, and four-beat) superimposed on a steady metronome while electroencephalography (EEG) measurements were made. Shared information between perception and imagery EEG is investigated by means of principal component analysis and by means of single-trial classification. RESULTS: Classification of accented from non-accented beats was possible with an average accuracy of 70% for perception and 61% for imagery data. Cross-condition classification yielded significant performance above chance level for a classifier trained on perception and tested on imagery data (up to 66%), and vice versa (up to 60%). CONCLUSIONS: Results show that detection of imagined accents is possible and reveal similarity in brain signatures relevant to distinction of accents from non-accents in perception and imagery. SIGNIFICANCE: Our results support the idea of shared mechanisms in perception and imagery for auditory processing. This is relevant for a number of clinical settings, most notably by elucidating the basic mechanisms of rhythmic auditory cuing paradigms, e.g. as used in motor rehabilitation or therapy for Parkinson's disease. As a novel Brain-Computer Interface (BCI) paradigm, our results imply a reduction of the necessary BCI training in healthy subjects and in patients.

Specific cellular and humoral immunity after immunization with live Towne strain cytomegalovirus vaccine.

Cytomegalovirus-specific lymphocyte proliferation and immunofluorescent antibody responses were measured serially in five normal adults and eight candidates for renal transplant immunized with Towne strain cytomegalovirus vaccine. All of the normal adults developed cellular and humoral immune responses. Five of the eight immunized renal transplant candidates developed proliferative responses comparable to those of normal adults, although in two instances maximal responses were delayed. Vaccinees developed proliferative responses to antigens prepared from the Towne strain as well as heterologous strains of cytomegalovirus. The appearance of immunofluorescent antibodies was generally delayed for the renal transplant candidates, and two of the individuals who had poor proliferative responses also had low antibody titers.