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2.
Eur J Pharm Sci ; 75: 60-71, 2015 Jul 30.
Article in English | MEDLINE | ID: mdl-25845631

ABSTRACT

Small interfering RNAs (siRNAs) have the potential to be of therapeutic value for many human diseases. So far, however, a serious obstacle to their therapeutic use is represented by the absence of appropriate delivery systems able to protect them from degradation and to allow an efficient cellular uptake. In this work we developed a siRNA delivery system based on inulin (Inu), an abundant and natural polysaccharide. Inu was functionalized via the conjugation with diethylenetriamine (DETA) residues to form the complex Inu-DETA. We studied the size, surface charge and the shape of the Inu-DETA/siRNA complexes; additionally, the cytotoxicity, the silencing efficacy and the cell uptake-mechanisms were studied in the human bronchial epithelial cells (16HBE) and in the hepatocellular carcinoma derived cells (JHH6). The results presented here indicate that Inu-DETA copolymers can effectively bind siRNAs, are highly cytocompatible and, in JHH6, can effectively deliver functional siRNAs. Optimal delivery is observed using a weight ratio Inu-DETA/siRNA of 4 that corresponds to polyplexes with an average size of 600nm and a slightly negative surface charge. Moreover, the uptake and trafficking mechanisms, mainly based on micropinocytosis and clatrin mediated endocytosis, allow the homogeneous diffusion of siRNA within the cytoplasm of JHH6. Notably, in 16 HBE where the trafficking mechanism (caveolae mediated endocytosis) does not allow an even distribution of siRNA within the cell cytoplasm, no significant siRNA activity is observed. In conclusion, we developed a novel inulin-based siRNA delivery system able to efficiently release siRNA in JHH6 with negligible cytotoxicity thus opening the way for further testing in more complex in vivo models.


Subject(s)
Inulin/administration & dosage , Polyamines/administration & dosage , RNA, Small Interfering/administration & dosage , Cell Line , Cell Line, Tumor , E2F1 Transcription Factor/genetics , Endocytosis , Humans , Inulin/chemistry , Polyamines/chemistry , RNA, Small Interfering/chemistry
3.
Eur J Pharm Sci ; 62: 326-33, 2014 Oct 01.
Article in English | MEDLINE | ID: mdl-24932712

ABSTRACT

This paper focuses on the micro- and nano-topological organization of a hydrogel, constituted by a mixture of bacterial cellulose and acrylic acid, and intended for biomedical applications. The presence of acrylic acid promotes the formation of two interpenetrated continuous phases: the primary "pores phase" (PP) containing only water and the secondary "polymeric network phase" (PNP) constituted by the polymeric network swollen by the water. Low field Nuclear Magnetic Resonance (LF NMR), rheology, Scanning Electron Microscopy (SEM) and release tests were used to determine the characteristics of the two phases. In particular, we found that this system is a strong hydrogel constituted by 81% (v/v) of PP phase the remaining part being occupied by the PNP phase. Pores diameters span in the range 10-100 µm, the majority of them (85%) falling in the range 30-90 µm. The high PP phase tortuosity indicates that big pores are not directly connected to each other, but their connection is realized by a series of interconnected small pores that rend the drug path tortuous. The PNP is characterized by a polymer volume fraction around 0.73 while mesh size is around 3 nm. The theoretical interpretation of the experimental data coming from the techniques panel adopted, yielded to the micro- and nano-organization of our hydrogel.


Subject(s)
Acrylates/chemistry , Cellulose/chemistry , Hydrogels/chemistry , Polymers/chemistry , Drug Liberation , Gluconacetobacter xylinus , Magnetic Resonance Spectroscopy , Microscopy, Electron, Scanning , Porosity , Rheology , Theophylline/chemistry
4.
Int J Pharm ; 466(1-2): 246-57, 2014 May 15.
Article in English | MEDLINE | ID: mdl-24631053

ABSTRACT

Nucleic acid molecules such as small interfering RNAs (siRNAs) and plasmidic DNAs (pDNAs) have been shown to have the potential to be of therapeutic value in different human diseases. Their practical use is however compromised by the lack of appropriate release systems. Delivered as naked molecules, siRNAs/pDNAs are rapidly degraded by extracellular nucleases thus considerably reducing the amount of molecule which can reach the target cells. Additionally, the anionic charge of the phosphate groups present on the siRNAs/pDNAs backbone, disfavors the interaction with the negatively charged surface of the cell membrane. In this paper we describe the generation of a novel polymer able to deliver both siRNAs and pDNAs. The combined release of these molecules is used in many different experimental settings such as the evaluation of the silencing efficiency of a given siRNA targeted against a given RNA, encoded by the pDNA. The possibility to use the same delivery system is very convenient from the technical point of view and it allows minimizing possible artifacts introduced by the use of different delivery agents for siRNAs and pDNA. The copolymer described here is based on α,ß-poly(N-2-hydroxyethyl)-d,l-aspartamide (PHEA) bearing positively chargeable side oligochains, with diethylamino ethyl methacrylate (DEAEMA) as monomer. Monomer polymerization has been obtained by atom transfer radical polymerization (ATRP), a technique which allows the precise polymerization of the monomer. In addition to the chemical-physical characterization of the polymer, we provide evidences of the polymer ability to delivery both siRNAs and pDNA to cultured cells. Whereas additional investigations are necessary to study the delivery mechanisms of this polyplex, the polymer generated represents a novel and convenient device for the delivery of both siRNAs and pDNA.


Subject(s)
DNA/chemistry , Methacrylates/chemistry , Peptides/chemistry , RNA, Small Interfering/chemistry , Animals , Cell Line, Tumor , Cell Survival/drug effects , DNA/administration & dosage , E2F1 Transcription Factor/genetics , Humans , Mice , Plasmids , Polymerization , RNA, Messenger/genetics , RNA, Small Interfering/administration & dosage , Transfection/methods
5.
Curr Med Chem ; 20(28): 3515-38, 2013.
Article in English | MEDLINE | ID: mdl-23745553

ABSTRACT

The thickening of the vessel wall (intimal hyperplasia) is a pathological process which often follows revascularization approaches such as transluminal angioplasty and artery bypass graft, procedures used to re-vascularize stenotic artery. Despite the significant improvements in the treatment of intimal hyperplasia obtained in the last years, the problem has not completely solved. Nucleic acid based-drugs (NABDs) represent an emergent class of molecules with potential therapeutic value for the treatment of intimal hyperplasia. NABDs of interest in the field of intimal hyperplasia are: ribozymes, DNAzymes, antisense oligonucleotides, decoy oligonucleotides, small interfering RNAs and micro interfering RNAs. These molecules can recognize, in a sequencespecific fashion, a target which, depending on the different NABDs, can be represented by a nucleic acid or a protein. Upon binding, NABDs can down-modulate the functions of the target (mRNA/proteins) and thus they are used to impair the functions of disease-causing biological molecules.In spite of the great therapeutic potential demonstrated by NABDs in many experimental model of intima hyperplasia, their practical use is hindered by the necessity to identify optimal delivery systems to the vasculature. In the first part of this review a brief description of the clinical problem related to intima hyperplasia formation after revascularization procedures is reported. In the second part, the attention is focused on the experimental evidences of NABD therapeutic potential in the prevention of intimal hyperplasia. Finally, in the third part, we will describe the strategies developed to optimize NABD delivery to the diseased vessel.


Subject(s)
Nucleic Acids/administration & dosage , Vascular Diseases/drug therapy , DNA, Catalytic/administration & dosage , DNA, Catalytic/chemistry , Drug Carriers/chemistry , Endothelium, Vascular/pathology , Humans , Hyperplasia , Nucleic Acids/chemistry , Oligonucleotides, Antisense/administration & dosage , Oligonucleotides, Antisense/chemistry , RNA, Catalytic/administration & dosage , RNA, Catalytic/chemistry , RNA, Small Interfering/administration & dosage , RNA, Small Interfering/chemistry
6.
Br J Cancer ; 106(1): 166-73, 2012 Jan 03.
Article in English | MEDLINE | ID: mdl-22095224

ABSTRACT

BACKGROUND: In prostate adenocarcinoma, the dissection of the expression behaviour of the eukaryotic elongation factors (eEF1A1/2) has not yet fully elucidated. METHODS: The EEF1A1/A2 expressions were investigated by real-time PCR, western blotting (cytoplasmic and cytoskeletal/nuclear-enriched fractions) and immunofluorescence in the androgen-responsive LNCaP and the non-responsive DU-145 and PC-3 cells, displaying a low, moderate and high aggressive phenotype, respectively. Targeted experiments were also conducted in the androgen-responsive 22Rv1, a cell line marking the progression towards androgen-refractory tumour. The non-tumourigenic prostate PZHPV-7 cell line was the control. RESULTS: Compared with PZHPV-7, cancer cells showed no major variations in EEF1A1 mRNA; eEF1A1 protein increased only in cytoskeletal/nuclear fraction. On the contrary, a significant rise of EEF1A2 mRNA and protein were found, with the highest levels detected in LNCaP. Eukaryotic elongation factor 1A2 immunostaining confirmed the western blotting results. Pilot evaluation in archive prostate tissues showed the presence of EEF1A2 mRNA in near all neoplastic and perineoplastic but not in normal samples or in benign adenoma; in contrast, EEF1A1 mRNA was everywhere detectable. CONCLUSION: Eukaryotic elongation factor 1A2 switch-on, observed in cultured tumour prostate cells and in human prostate tumour samples, may represent a feature of prostate cancer; in contrast, a minor involvement is assigned to EEF1A1. These observations suggest to consider EEF1A2 as a marker for prostate cell transformation and/or possibly as a hallmark of cancer progression.


Subject(s)
Cell Transformation, Neoplastic/genetics , Peptide Elongation Factor 1/genetics , Prostatic Neoplasms/genetics , Base Sequence , Blotting, Western , Cell Line, Tumor , DNA Primers , Fluorescent Antibody Technique , Humans , Male , Paraffin Embedding , Prostatic Neoplasms/pathology , RNA, Messenger/genetics , Real-Time Polymerase Chain Reaction , Reverse Transcriptase Polymerase Chain Reaction
7.
Biochimie ; 89(12): 1544-52, 2007 Dec.
Article in English | MEDLINE | ID: mdl-17825975

ABSTRACT

Despite the involvement of the elongation factors eEF1A (eEF1A1 and eEF1A2) in the development of different cancers no information is available on their possible contribution to the biology of hepatocellular carcinoma (HCC). We investigated the expression of both forms of eEF1A in HepG2 and JHH6 cell lines considered to be a good in vitro model of HCC at different stage of differentiation. Our data indicate that the mRNA amount of eEF1A1 is increased in both cell lines as compared to normal liver tissue, but eEF1A2 mRNA level is markedly increased only in JHH6. Moreover, the less differentiated cell line JHH6 displays higher EEF1A1 and EEF1A2 mRNAs levels and an higher nuclear-enriched/cytoplasm ratio of EEF1A protein compared to the better differentiated HepG2 cell line. Over-expression depends only partially on gene amplification. The more abundant mRNA levels and the higher nuclear-enriched/cytoplasm ratio of eEF1A in JHH6 neither correlate with apoptosis resistance nor with proliferation rate in sub-confluent cells. However, in confluent cells, a clear tendency to maintain JHH6 into the cell cycle was observed. In conclusion, we document the increased mRNA levels of EEF1A genes in HCC cell lines compared to normal liver. Additionally, we show the increased nuclear-enriched/cytoplasmic protein ratio of eEF1A and the marked raise of the expression of both eEF1A forms in JHH6 compared to HepG2, suggesting the possibility that eEF1A forms might become a relevant markers related to HCC tumor phenotype.


Subject(s)
Apoptosis , Cell Differentiation , Cell Proliferation , Peptide Elongation Factor 1/metabolism , Blotting, Western , Carcinoma, Hepatocellular/pathology , Cell Culture Techniques , Cell Line, Tumor , DNA, Complementary/biosynthesis , Gene Amplification , HeLa Cells , Humans , Liver Neoplasms/pathology , RNA, Messenger/analysis
8.
J Colloid Interface Sci ; 301(1): 282-90, 2006 Sep 01.
Article in English | MEDLINE | ID: mdl-16777132

ABSTRACT

Rheological and erosion studies regarding a liposome-containing polymeric blend that is propaedeutic to its use in paving techniques in tubular organs, such as blood vessels, are reported. Attention is focused on an aqueous polymeric blend composed of Pluronic (PF127) and alginate (Protanal LF 10/60) because both polymers, when dissolved in water at a sufficiently high concentration, are subjected to different structural mechanisms, which are driven by temperature increase and addition of bivalent cations, respectively, and both result in marked viscoelastic and plastic properties. After proving the compatibility between PF127 and alginate, we show that the structural transition temperature of the blend, T(ST), can be properly modulated. In particular, we found that T(ST) for an aqueous solution of pure Pluronic 20% w/w is about 21 degrees C and that even slight reductions in polymer concentration result in considerable T(ST) decrease. The addition of salts or alginate (provided as Na-alginate) provokes a substantial decrease of T(ST) and thus the alginate concentration in the blend should not exceed 1% w/w. In addition, liposomes slow down the structural transition but do not substantially affect the rheological properties of the system in the final state at higher temperatures, thus showing that they can be added to the polymeric blend without significant effects. Finally, erosion tests show that after contact with a source of bivalent cations, the polymeric blend containing PF127 and alginate shows an erosion resistance neatly improved with respect to the simple structured Pluronic system having the same polymer concentration. As a whole, all these results constitute the basis for future potential applications of the considered polymeric blend in tubular organs such as blood vessels.


Subject(s)
Alginates/chemistry , Biocompatible Materials/chemistry , Drug Delivery Systems , Liposomes/chemistry , Poloxamer/chemistry , Water/chemistry , Glucuronic Acid/chemistry , Hexuronic Acids/chemistry , Rheology , Temperature , Viscosity
9.
Arch Virol ; 151(2): 255-71, 2006 Feb.
Article in English | MEDLINE | ID: mdl-16195789

ABSTRACT

The development of effective gene-therapeutic applications for cardiovascular disorders is in part limited by the lack of appropriate delivery systems. In an attempt to overcome this deficiency, we investigated the ability of baculoviral vectors to transduce human cardiovascular cells, for which data are missing in literature. Additionally, baculovirus ability to transduce target cells was compared to that of an adenoviral vector, a well characterized and widely used viral vector. Transduction experiments, performed using baculo/adenoviral vectors expressing the enhanced green fluorescence protein, revealed that, under the experimental condition considered, baculoviruses but not adenoviruses efficiently transduce human coronary smooth muscle cells (hCSMC); an opposite behavior was noticed for human coronary endothelial cells (hCEC). Thus, baculoviral vectors are potentially indicated as transfer system in the treatment of coronary restenosis, where growth inhibitory genes should reach hCSMC but not hCEC. When used to transduce human cardiomyocytes and fibroblasts, both vectors behaved similarly. Finally, studies on cellular DNA replication revealed a more prolonged and pronounced negative effect on cells transduced by adenoviral compared to baculoviral vectors. Our data indicate that baculoviruses represent an attractive alternative to adenoviruses as transfer vectors in cardiovascular cells and that baculovirus have the potential to be used as gene transfer system in cardiovascular diseases such as restenosis.


Subject(s)
Adenoviridae/genetics , Baculoviridae/genetics , Cardiovascular System/cytology , DNA, Recombinant/genetics , Genetic Vectors/genetics , Transduction, Genetic/methods , Animals , Cell Line , Coronary Vessels/cytology , Endothelial Cells/metabolism , Fibroblasts , Gene Expression Regulation , Humans , Insecta , Muscle, Smooth, Vascular/metabolism , Myocytes, Cardiac/metabolism , Myocytes, Smooth Muscle/metabolism , Swine
11.
Int J Nanomedicine ; 1(4): 523-33, 2006.
Article in English | MEDLINE | ID: mdl-17722283

ABSTRACT

We studied the mechanism governing the delivery of nucleic acid-based drugs (NABD) from microparticles and nanoparticles in zero shear conditions, a situation occurring in applications such as in situ delivery to organ parenchyma. The delivery of a NABD molecule from poly(DL-lactide-co-glycolide) (PLGA) microparticles and stearic acid (SA) nanoparticles was studied using an experimental apparatus comprising a donor chamber separated from the receiver chamber by a synthetic membrane. A possible toxic effect on cell biology, as evaluated by studying cell proliferation, was also conducted forjust PLGA microparticles. A mathematical model based on the hypothesis that NABD release from particles is due to particle erosion was used to interpret experimental release data. Despite zero shear conditions imposed in the donor chamber, particle erosion was the leading mechanism for NABD release from both PLGA microparticles and SA nanoparticles. PLGA microparticle erosion speed is one order of magnitude higher than that of competing SA nanoparticles. Finally, no deleterious effects of PLGA microparticles on cell proliferation were detected. Thus, the data here reported can help optimize the delivery systems aimed at release of NABD from micro- and nanoparticles.


Subject(s)
Lactic Acid/chemistry , Lactic Acid/pharmacology , Myocytes, Smooth Muscle/drug effects , Nanostructures/chemistry , Nucleic Acids/administration & dosage , Polyglycolic Acid/chemistry , Polyglycolic Acid/pharmacology , Polymers/chemistry , Polymers/pharmacology , Stearic Acids/chemistry , Stearic Acids/pharmacology , Cells, Cultured , Computer Simulation , Drug Carriers/chemistry , Drug Carriers/pharmacology , Humans , Materials Testing , Microspheres , Models, Chemical , Myocytes, Smooth Muscle/cytology , Nanostructures/ultrastructure , Nucleic Acids/chemistry , Polylactic Acid-Polyglycolic Acid Copolymer
13.
Diagn Microbiol Infect Dis ; 29(3): 133-8, 1997 Nov.
Article in English | MEDLINE | ID: mdl-9401805

ABSTRACT

A retrospective review of laboratory records from 1988 to 1996 has shown an increased rate of ciprofloxacin-resistant (cip(r)) Escherichia coli in our rehabilitation center. Resistance increased from 0.6% in 1989 to 5.9% in 1996. Of 7870 E. coli strains isolated during this period, 257 cip(r)-E. coli were recovered from 257 patients. The majority (96%) of these resistant strains were isolated from the urine samples. One hundred and twenty strains of cip(r)-E. coli were also resistant to four other fluoroquinolones. MICs ranging from 64 to 512 micrograms/mL were observed in 75% of the strains and > or = 1028 micrograms/mL in 6.4% of the strains. Resistance to ciprofloxacin was due to possible mutations in topoisomerase gyrA.


Subject(s)
Anti-Infective Agents/pharmacology , Ciprofloxacin/pharmacology , Escherichia coli Infections/drug therapy , Escherichia coli Infections/microbiology , Escherichia coli/drug effects , Bacteriuria/drug therapy , Bacteriuria/epidemiology , Bacteriuria/microbiology , DNA Gyrase , DNA Topoisomerases, Type II/genetics , Drug Resistance, Microbial , Escherichia coli/enzymology , Escherichia coli/genetics , Escherichia coli Infections/epidemiology , Genes, Bacterial , Humans , Los Angeles/epidemiology , Microbial Sensitivity Tests , Mutation , Rehabilitation Centers , Retrospective Studies , Time Factors
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