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FcUni-RLuc: an engineered Renilla luciferase with Fc binding ability and light emission activity.

Farzannia, A; Roghanian, R; Zarkesh-Esfahani, S H; Nazari, M; Emamzadeh, R.

Analyst ; 140(5): 1438-41, 2015 Mar 07.

Article in English | MEDLINE | ID: mdl-25622753

ABSTRACT

A novel and advanced Fc-binding probe FcUni-RLuc namely has been produced and functionally assayed for labelling IgGs. The Fc antibody binding sequence HWRGWV was fused to Renilla luciferase, and the purified probe was employed for bioluminescence enzyme-linked immunoabsorbance assay of Her2 positive cells.

Subject(s)

Antibodies, Monoclonal, Humanized/metabolism , Breast Neoplasms/diagnosis , Immunoglobulin Fc Fragments/metabolism , Luciferases, Renilla/metabolism , Molecular Probes , Protein Engineering , Receptor, ErbB-2/metabolism , Antibodies, Monoclonal, Humanized/genetics , Biological Assay/methods , Breast Neoplasms/metabolism , Female , Humans , Immunoglobulin Fc Fragments/genetics , Luciferases, Renilla/genetics , Luminescent Measurements/methods , Protein Binding , Receptor, ErbB-2/genetics , Signal Transduction , Trastuzumab , Tumor Cells, Cultured

ABSTRACT

Subject(s)

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