ABSTRACT
Melanoma is one of the most malignant tumors, which leaves no chance of survival in the case of the "bang". There are various ways to treat tumors, however, recently in the field of cancer research, there are studies in which fungal metabolites have been used as antitumor agents. In this study we examined the effect of the culture fluid of the fungus Trichoderma asperellum 302 on the growth and development of melanoma B 16. We have shown that these culture fluid has anticancer properties, causing destruction of tumor tissue. Obtained data open new possibilities and prospects for the use of active substances derived from fungi in the complex therapy of cancer.
Subject(s)
Antineoplastic Agents/pharmacology , Culture Media, Conditioned/pharmacology , Melanoma, Experimental/drug therapy , Skin Neoplasms/drug therapy , Soil Microbiology , Trichoderma/chemistry , Animals , Body Weight/drug effects , Doxorubicin/pharmacology , Female , Hyalin/drug effects , Melanoma, Experimental/pathology , Mice , Mice, Inbred C57BL , Skin Neoplasms/pathology , Trichoderma/isolation & purification , Trichoderma/metabolismABSTRACT
2D matrix for cells cultivation has been created using ionic plasma processing of silicate glass. We have shown that the CD-1 mouse hepatocytes are adsorbed on the modified and the control glass after 24 h cultivation. Number of these cells on the modified glass was 2.8-0.2 more as compared with that in control on a smooth glass. Human fibroblasts adsorbed on a modofied glass matrix had normal configuration and their number was 1.5 ± 0.5 times greater that the number of cells in the control of smooth glass matrix.
Subject(s)
Fibroblasts/cytology , Glass/analysis , Hepatocytes/cytology , Animals , Cell Adhesion/physiology , Fibroblasts/physiology , Hepatocytes/physiology , Humans , Male , Mice , Plasma Gases/chemistry , Primary Cell Culture , Static Electricity , Surface Properties , Tissue ScaffoldsABSTRACT
Effects of dimephosphone, xydiphone, and ionol administered in parallel with phenobarbital on the content of cytochromes P-450 in rat liver and on the rate of C-hydroxylation of diazepam, haloperidol, and prednisolone by rat liver microsomal enzymes were studied in vitro. Dimephosphone, xydiphone, and ionol exhibited similar inductive effects on C-hydroxylation reactions in the CYP P-450 system during treatment with phenobarbital. Xydiphone and ionol in a dose of 1 mmol/kg canceled phenobarbital-induced increase in P-450 cytochrome content in rat liver. Sex-dependent cytochromes P-450 are involved in the prednisolone and haloperidol C-hydroxylation reactions in rats.
Subject(s)
Butylated Hydroxytoluene/pharmacology , Cytochrome P-450 Enzyme System/metabolism , Etidronic Acid/pharmacology , Microsomes, Liver/drug effects , Microsomes, Liver/enzymology , Organophosphorus Compounds/pharmacology , Animals , Diazepam/metabolism , Female , Haloperidol/metabolism , Hydroxylation , In Vitro Techniques , Kinetics , Male , Phenobarbital/pharmacology , Prednisolone/metabolism , Rats , Sex CharacteristicsABSTRACT
The mutagenic activity of 16 industrial nonionic surfactant samples has been investigated in Ames-test without metabolic activation and with the use of mouse liver microsomal fraction in vitro. The genotoxical estimation of their possible biodegradation products is given. The mutagenicity of a number of high molecular polyethylene glycoles related to nonionic surfactants is shown.
Subject(s)
Genes, Bacterial/drug effects , Salmonella typhimurium/drug effects , Surface-Active Agents/toxicity , Biodegradation, Environmental , Dose-Response Relationship, Drug , Mutagenicity Tests/methods , Salmonella typhimurium/geneticsABSTRACT
The yeast Rhodotorula mucilaginosa was able to grow in media containing triethanolamine or diethanolamine as the sole nitrogen source. During growth in the presence of triethanolamine, extracts of yeast cells contained increased levels of cytochrome P-450 dependent monooxygenase which catalyzed the oxidative N-dealkylation of aminoalcohols. Formation of diethanolamine, ethanolamine and glyoxylate from triethanolamine was demonstrated, and the identity of the products was verified by thin layer chromatography. These observations suggested the following scheme of triethanolamine catabolism: triethanolamine----diethanolamine + glycolaldehyde, diethanolamine----ethanolamine + glycolaldehyde, ethanolamine----NH3 + glycolaldehyde----glycolate----glyoxylate----glycerate pathway.
