ABSTRACT
A case detailing the successful management of aortic graft sepsis resulting from Pasteurella multocida is reported. The infection was the result of a bite from the patient's dog.
Subject(s)
Aortic Diseases/etiology , Blood Vessel Prosthesis , Equipment Contamination , Pasteurella Infections/etiology , Postoperative Complications/etiology , Aged , Animals , Aorta, Abdominal/surgery , Aortic Diseases/surgery , Bites and Stings/complications , Dogs , Female , Humans , Pasteurella Infections/surgery , Postoperative Complications/surgery , ReoperationABSTRACT
1. The stereoisomers of delta-(alpha-aminoadipyl)-L-cysteinylvaline (LLD, LLL and DLD) were synthesized from valine labelled with 3H in its methyl groups or in the alpha position. L-Cysteinyl-D-[4,4'-3H]valine was also synthesized. 2. 3H was incorporated into a compound that behaved like penicillin N when the LLD tripeptide containing either a methyl- or an alpha-labelled valine residue was incubated with a cell-free system prepared by lysis of protoplasts of Cephalosporium acremonium. 3. Incorporation was not observed under these conditions from the labelled all-L- or DLD-tripeptide, from L-cysteinyl-D-[4,4'-3H]valine, or of Penicillium chrysogenum appeared to be the LLD isomer, like that from C. acremonium. 5. These findings are discussed in relation to penicillin biosynthesis.
Subject(s)
Oligopeptides/chemical synthesis , Penicillins/biosynthesis , Acremonium/metabolism , Cell-Free System , Cysteine , Dipeptides/chemical synthesis , Penicillium chrysogenum/metabolism , Protoplasts , Stereoisomerism , Tritium , Valine/chemical synthesisABSTRACT
1. 3H was incorporated into solvent-soluble penicillin from isopenicillin N and 6-aminopenicillanic acid 3H-labelled in the 2beta-methyl group when the labelled compounds were incubated with a crude extract of Penicillum chrysogenum. 2. With a soluble protein fraction of the extract incorporation from isopenicillin N occurred on addition of phenyl-acetyl-CoA. 3. Labelled benzylpenicillin was isolated after incubation of the crude extract with phenylacetyl-CoA and isopenicillin and the addition of unlabelled benzylpenicillin as a carrier. 4. No incorporation of 3H into solvent-soluble penicillin was detected on incubation of these extracts with penicillin N.
