ABSTRACT
INTRODUCTION: The development of uterine transplantation (UTx) from deceased donors requires knowledge of the tolerance of the uterus to prolonged cold ischemia (CI). This can be evaluated through the use of biological parameters to assess degradation of the organ between its procurement and transplantation. The objective of this study was to analyze changes in the metabolic composition of the storage solution in cases of prolonged CI in uteri from ewes. METHODS: Eighteen uterine auto-transplantations were performed in ewes. CI time was 1 h (T1) or 24 h (T24). Samples of Celsior® were taken when the explanted uterus was flushed (T0) and at the end of CI. A dual approach to metabolic analyses was followed: targeted biochemical analyses targeting several predefined metabolites and non-targeted metabolomics analyses based on nuclear magnetic resonance (NMR). RESULTS: Metabolic analyses were performed on 16 explanted uteri. Metabolomic profiles differed significantly between T1 and T24 (p = 0.003). Hypoxia-associated degradation of the organ was demonstrated by the significantly higher lactate levels at T24 than at T1 (p < 0.05), accompanied by cell lysis, and significantly higher levels of creatine kinase activity in T24 than in T1 uteri (p < 0.05). Oxidative stress increased over time, with a significantly higher oxidized glutathione/glutathione ratio for T24 than for T1 uteri (p < 0.05). CONCLUSION: The metabolic results indicate a significant degradation of the uterus during 24 h of CI. Metabolic analysis of the storage solution could be used as a non-invasive tool for evaluating uterine degradation during CI before transplantation.
Subject(s)
Metabolome/genetics , Oxidative Stress/physiology , Transplantation, Autologous , Uterus/metabolism , Animals , Cold Ischemia/methods , Female , Humans , Magnetic Resonance Spectroscopy , Models, Animal , Sheep , Tissue Donors , Uterus/physiologyABSTRACT
L'etude retrospective a porte sur une periode de cinq annees; de janvier 1987 a decembre 1991. elle a recense 199 enuretiques sur 1009 consultants a la clinique pedopsychiatrique. L'enuresie represente 20 pour cent de cette consultation. La majorite des cas viennent a Ker Xaleyi vers l'age de 12 ans qui correspond au pic. L'affection est relativement plus frequente chez les patients feminins 23 pour cent que masculins 17;33 pour cent
