ABSTRACT
A current challenge for tissue engineering while restoring the function of diseased or damaged tissue is to customize the tissue according to the target area. Scaffold-free approaches usually yield spheroid shapes with the risk of necrosis at the center due to poor nutrient and oxygen diffusion. Here, we used magnetic forces developed at the cellular scale by miniaturized magnets to create rod-shaped aggregates of stem cells that subsequently matured into a tissue-like structure. However, during the maturation process, the tissue-rods spontaneously bent and coiled into sphere-like structures, triggered by the increasing cell-cell adhesion within the initially non-homogeneous tissue. Optimisation of the intra-tissular magnetic forces successfully hindered the transition, in order to produce stable rod-shaped stem cells aggregates.
Subject(s)
Mesenchymal Stem Cells/cytology , Tissue Engineering/methods , Biophysical Phenomena , Cell Adhesion , Cell Aggregation , Cells, Cultured , Humans , Magnetic Phenomena , Miniaturization , Spheroids, Cellular/cytology , Tissue Engineering/instrumentationABSTRACT
Magnetic forces induce cell condensation necessary for stem cell differentiation into cartilage and elicit the formation of a tissue-like structure: Magnetically driven fusion of aggregates assembled by micromagnets results in the formation of a continuous tissue layer containing abundant cartilage matrix.
Subject(s)
Cartilage/cytology , Cell Differentiation , Magnetic Phenomena , Mesenchymal Stem Cells/cytology , Stem Cells/cytology , Cell Aggregation , Chondrogenesis , Citrates/chemistry , Humans , Magnetite Nanoparticles/chemistryABSTRACT
We report a new case of granuloma inguinale in France. A 47 years old Turkish man living in France for eight years had an ulcerovegetative lesion on the corona sulcus and inner aspect of the prepuce for three months. Donovanosis was established by granulation time smears and Giemsa's stain showing Donovan bodies inside large mononuclear cells. Complete resolution occurred after treatment with ampicillin for three weeks and later doxycycline for a month. Granuloma inguinale is rare in France and must not be forgotten. Epidemiologic data, clinical manifestations and therapeutic indications are reviewed.
