1.
Biochemistry (Mosc)
; 71(4): 423-9, 2006 Apr.
Article
in English
| MEDLINE
| ID: mdl-16615862
ABSTRACT
Brassinosteroid-induced phosphorylation of tyrosine residues in proteins was studied. Proteins of crude extract of pea leaves were analyzed by one- and two-dimensional electrophoresis followed by Western blotting with monoclonal antibodies PY20 to phosphotyrosine proteins. One- and two-dimensional electrophoresis revealed 7 and 13 tyrosine-phosphorylated proteins, respectively. Brassinolide increased the phosphorylation level of most of these proteins. With inhibitors of tyrosine protein phosphatases, such as phenylarsine oxide and orthovanadate, the level of tyrosine phosphorylation of these proteins increased.
Subject(s)
Cholestanols/pharmacology , Pisum sativum/metabolism , Plant Growth Regulators/pharmacology , Plant Proteins/metabolism , Steroids, Heterocyclic/pharmacology , Tyrosine/metabolism , Brassinosteroids , Cholestanols/metabolism , Electrophoresis, Gel, Two-Dimensional , Molecular Weight , Pisum sativum/cytology , Phosphorylation , Plant Growth Regulators/metabolism , Plant Leaves/cytology , Plant Leaves/drug effects , Plant Leaves/metabolism , Steroids, Heterocyclic/metabolism
2.
Dokl Biochem Biophys
; 395: 80-3, 2004.
Article
in English
| MEDLINE
| ID: mdl-15253557