ABSTRACT
In Wistar rats with transplanted Walker 256 carcinosarcoma a use of melatonin as monoagent causes changes in intracellular organization of endothelial cells: reduced volume density of mitochondria, granular cytoplasmic network, micropinocytic vesicles, reduced the number density of free and attached ribosomes, which leads to increased apoptosis of tumor cells of Walker 256 carcinosarcoma.
Subject(s)
Antineoplastic Agents/pharmacology , Apoptosis/drug effects , Capillaries/pathology , Carcinoma 256, Walker/pathology , Endothelial Cells/ultrastructure , Melatonin/pharmacology , Animals , Antineoplastic Agents/administration & dosage , Carcinoma 256, Walker/ultrastructure , Cytoplasm/drug effects , Male , Melatonin/administration & dosage , Mitochondria/drug effects , Pinocytosis/drug effects , Rats , Ribosomes/drug effectsABSTRACT
Whole-body controlled hyperthermia (up to 43.5 degrees C) of Wistar rats with Walker-256 carcinosarcoma was followed by symptoms of enhanced lipid peroxidation for 3-14 days and release of lysosomal enzymes into blood on day 14. Our data, in totality, point to a potential to stimulate tumor cell apoptosis.
Subject(s)
Carcinoma 256, Walker/metabolism , Carcinoma 256, Walker/therapy , Hyperthermia, Induced , Lipid Peroxidation , Lysosomes/enzymology , Animals , Apoptosis , Carcinoma 256, Walker/enzymology , Carcinoma 256, Walker/pathology , Female , Rats , Rats, WistarABSTRACT
Threefold administration of benz(a)pyrene in a dose of 20 mg/kg markedly stimulated biliary excretion of lysosomal enzymes from rat liver without signs of lysosomal damage. Constant light exposure induced changes attesting to functional activation of the lysosomal apparatus in liver cells and inhibited constitutive biliary excretion of lysosomal enzymes. Combined treatment decreased, but not abolished the stimulatory effect of benz(a)pyrene on vesicular transport of lysosomal enzymes to the bile.
