ABSTRACT
Conjugate the fluorescent dye 4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indatsen-8-propionic acid (BODIPY) and N2,N4,N6-trimethylmelamine was obtained. It was shown that this compound in the presence of formaldehyde generates covalent cross-links of DNA strands in vitro.
Subject(s)
Antineoplastic Agents/chemical synthesis , Boron Compounds/chemical synthesis , Cross-Linking Reagents/chemical synthesis , Fluorescent Dyes/chemical synthesis , Triazines/chemical synthesis , Animals , Antineoplastic Agents/therapeutic use , DNA/chemistry , Humans , Neoplasms/drug therapy , Spectrometry, FluorescenceABSTRACT
Published data on the main types of reagents capable of introducing covalent interstrand cross links into nucleic acids (NA) are summarized in the present review. The reactivity of cross-linking agents, their preferred binding sites, and methods of determining the cross-link localization in a duplex are discussed. Cell response to DNA cross linking, namely, the blocking of replication and transcription, the initiation of reparation processes, and apoptotic death of the cell, are analyzed, as well as the use of cross-linking reagents in therapy and molecular biology.
Subject(s)
Cross-Linking Reagents , DNA , RNA , Alkylating Agents/chemistry , Alkylating Agents/pharmacology , Alkylation , Animals , Antineoplastic Agents/chemistry , Antineoplastic Agents/pharmacology , Apoptosis , Cross-Linking Reagents/chemistry , Cross-Linking Reagents/metabolism , Cross-Linking Reagents/pharmacology , DNA/chemistry , DNA/metabolism , Humans , Nucleic Acid Heteroduplexes/chemistry , Nucleic Acid Heteroduplexes/metabolism , RNA/chemistry , RNA/metabolismABSTRACT
A rapid and effective method of an automatic oligoribonucleotide synthesis alternative to the phosphoroamidite one was developed based on the phosphotriester approach of internucleotide bond formation under intramolecular O-nucleophilic catalysis and the use of an azidomethyl group for protection of a nucleotide 2'-hydroxyl function.
