ABSTRACT
The cell wall glycopolymers of three strains of Streptomyces albus and the type strain of Streptomyces pathocidini were investigated. The structures of the glycopolymers were established using a combination of chemical and NMR spectroscopic methods. The cell wall of S. albus subsp. albus VKM Ac-35(T) was found to be comprised of three glycopolymers, viz. unsubstituted 1,5-poly(ribitol phosphate), 1,3-poly(glycerol phosphate) substituted with ß-D-glucopyranose, and the major polymer, a 3-deoxy-D-glycero-D-galacto-non-2-ulosonic acid (Kdn)-teichulosonic acid: ß-D-Glcp-(1 â 8)-α-Kdnp-(2[(â6)-ß-D-Glcp-(1 â 8)-α-Kdnp-(2 â] n 6)-ß-D-Glcp-(1 â 8)-ß-Kdnp-(2-OH, where n ≥ 3. The cell walls of 'S. albus' J1074 and 'S. albus' R1-100 were found to contain three glycopolymers of identical structures, viz. unsubstituted 1,3- and 2,3-poly(glycerol phosphates), and the major polymer, a Kdn-teichulosonic acid with an unusual structure that has not been previously described: ß-D-Galp-(1 â 9)-α-Kdnp-(2[(â3)-ß-D-Galp-(1 â 9)-α-Kdnp-(2 â] n 3)-ß-D-Galp-(1 â 9)-ß-Kdnp-(2-OH, where n ~ 7-8. The cell wall of S. pathocidini (formerly S. albus subsp. pathocidicus) VKM Ac-598(T) was found to contain two glycopolymers, viz. 1,3-poly(glycerol phosphate) partially O-glycosylated with 2-acetamido-2-deoxy-α-D-glucopyranose and/or O-acylated with L-lysine, and a poly(diglycosyl 1-phosphate) of hitherto unknown structure: -6)-α-D-Glcp-(1 â 6)-α-D-GlcpNAc-(1-P-.
Subject(s)
Polysaccharides, Bacterial/metabolism , Streptomyces/metabolism , Cell Wall/metabolism , Lysine/metabolism , Magnetic Resonance Spectroscopy/methods , Phosphates/isolation & purification , Phosphates/metabolism , Polysaccharides, Bacterial/isolation & purification , Streptomyces/chemistry , Sugar Acids/isolation & purification , Sugar Acids/metabolism , Teichoic Acids/isolation & purification , Teichoic Acids/metabolism , Uronic Acids/isolation & purification , Uronic Acids/metabolismABSTRACT
The cell wall of Streptomyces coelicolor M145, a prototrophic plasmidless (SCP1(-) SCP2(-)) variant of strain S. coelicolor A3(2) contains the main glycopolymer represented by Kdn-containing teichulosonic acid with unusual structure which has not been described so far: [structure: see text]. The minor polymer was found to be a poly(diglycosyl 1-phosphate) with the following repeating unit: -6)-α-Galp-(1â6)-α-GlcpNAc-(1-P-. The structures of both glycopolymers were established by using a combination of chemical and NMR spectroscopic methods.
