ABSTRACT
The intensity of immunity, depending on immune response variants characterized by the definite composition of the T and B lymphocyte subpopulation in peripheral blood, was studied in 70 practically healthy young children after the primary complex of immunization with adsorbed DPT vaccine. The most stable immune reaction was shown to appear in children with the hyperergic and normoergic variants of immune response to diphtheria and tetanus toxoids, while the reaction to pertussis antigen was essentially weaker both after the second vaccination and after the first revaccination. In children with the hypoergic variant of immune response to all components of adsorbed DPT vaccine the resulting immune reaction was 2.5-3 times weaker than in children of other groups.
Subject(s)
Diphtheria-Tetanus-Pertussis Vaccine/immunology , Adsorption , Antibodies, Bacterial/blood , Antibody Specificity , B-Lymphocytes/immunology , Bordetella pertussis/immunology , Clostridium tetani/immunology , Corynebacterium diphtheriae/immunology , Humans , Immunity, Cellular , Immunization Schedule , Immunization, Secondary/statistics & numerical data , Infant , Statistics, Nonparametric , T-Lymphocytes/immunologyABSTRACT
The dynamics of the intensity of specific antidiphtheria immunity after the second age-scheduled revaccination was studied in 129 practically healthy children. The study revealed that the formation of immunity depended on the initial functional state of the immune system before the injection of diphtheria toxoid. Three variants of immune response were determined and the immune status corresponding to each of these variants was characterized. As shown in this study, children with the hyperergic character of immune response were characterized by relatively high initial titers of antitoxin, and the injection of an additional dose of the antigen led to the prolonged state of hyperimmunization with the subsequent decrease of the intensity of immunity by half, registered in the catamnestic observation for 4 years. Children with the hypo- and normoergic variants of immune response were characterized by the most stable immune response to diphtheria toxoid, and during the catamnestic observation they formed the levels of antibody titers 2.5- to 3-fold higher than before immunization. But the protection characteristics in children with the third variant were the lowest among the children under study.
Subject(s)
Diphtheria Toxoid/immunology , Immunization, Secondary , Antibody Formation , Child , Humans , Immunity, CellularABSTRACT
Different strains of mice CBA (H-2k) and C57BL/6 (H-2b) have been exposed to burn of flame of 15% skin-deep of body. Spleen cells were received 6 hours, 3 and 7 days after burn trauma of mice in order to induce the local graft-versus-host reaction by subcutaneous injection of 10(7) cells in foot of hind leg of (CBA X C57BL/6)F1 hybrids. The enhancement of the ability of spleen cells to induce graft-versus host was observed in 6 hours and 3 days. The burn trauma of C57BL/6 mice produced the suppression of reactivity of spleen cells in graft-versus-host reaction for all terms of research. The donors of spleen cells adrenalectomy 5 days before the burn trauma eliminated differences of reactivity of lymphocytes of mice CBA and C57BL/6.
Subject(s)
Burns/immunology , Graft vs Host Reaction , Spleen/immunology , Adrenalectomy , Animals , Male , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , Spleen/cytology , Spleen/transplantationABSTRACT
It has been shown that trophoblastic beta 1-glycoprotein (TBG) and placental alpha 2-microglobulin (PAMG-2) in concentrations 60-120 micrograms/ml suppresses both the inductive and proliferative phase of unidirectional mixed lymphocyte reaction in mice, as well as proliferative responses to phytohemagglutinin or pokeweed mitogen. TBG protein was more effective. The proteins were not toxic for lymphocytes.
Subject(s)
Alpha-Globulins/pharmacology , Immunosuppressive Agents/pharmacology , Pregnancy Proteins/pharmacology , Pregnancy-Specific beta 1-Glycoproteins/pharmacology , Animals , Cell Survival/drug effects , Depression, Chemical , Humans , Lymphocyte Activation/drug effects , Lymphocyte Culture Test, Mixed , Male , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Mice, Inbred CBA , Time FactorsABSTRACT
Triiodothyronine (T3), thyroxine (T4) and thyroid stimulating hormone (TSH) serum content was measured in mice during systemic "graft-versus-host" reaction (GVHR), using radioimmunoassay. It was demonstrated that on the 3rd day after GVHR induction the levels of these hormones did not differ from the control values. T3 and T4 concentrations and 125I absorption by thyroid gland diminished by day 10. At the same time TSH level remained unchanged. On day 24 after GVHR induction T3 and T4 content was significantly reduced, although TSH concentration exceeded the control value. 125I absorption was enhanced as compared to the value observed on day 10. The data obtained show the vigorous inhibition of thyroid gland function during systemic GVHR.
Subject(s)
Graft vs Host Reaction , Thyroid Gland/physiology , Animals , Iodine/metabolism , Male , Mice , Organ Size , Thyrotropin/blood , Thyroxine/blood , Triiodothyronine/bloodABSTRACT
The development of the graft-versus-host reaction (GVHR) was studied in female (CBAxC57BL)F1 mice during pregnancy, after delivery or before mating with syngeneic, semisyngeneic and allogeneic males. It was found that the results in females depend on genetic differences between the donors of injected lymphoid cells and fetuses, as well as on the time of inducing GVHR. If lymphoid cells from C57BL/L mice were injected to (CBA X C57BL) females after delivery or before mating with CBA males of the parental strain, the gravidity promoted the enhancement of GVHR. Infection of lymphoid cells during pregnancy produced marked increases resistance ot GVHR. In case of mating with males of the contralateral paternal strain C57BL/6, gravidity did not influence GVHR irrespective the injection time.
