ABSTRACT
Placentas from women aged 25-32 years with normal course of gestation were studied. It is essential to stick to certain methodological approaches for preparing viable multipotent mesenchymal stromal cell culture and to carry out morphological (macro and micro) evaluation of the chorionic villi, umbilical cords, and placentas. At stage I of the study, patients' histories, labor course, and examinations of the newborns should be analyzed to exclude women with genital and extragenital diseases. At stage II, it is essential to stick to special regulations and methods for collection of specimens of the cord, amnion, and placental tissue proper. Histological control of the placental structures collected for multipotent mesenchymal stromal cell culturing is obligatory.
Subject(s)
Amnion/cytology , Mesenchymal Stem Cells/cytology , Multipotent Stem Cells/cytology , Placenta/cytology , Specimen Handling/standards , Umbilical Cord/cytology , Adult , Amnion/metabolism , Antigens, CD/genetics , Antigens, CD/metabolism , Biomarkers/metabolism , CDC2 Protein Kinase , Cell Differentiation , Chorionic Villi/metabolism , Chorionic Villi/ultrastructure , Cyclin B1/genetics , Cyclin B1/metabolism , Cyclin-Dependent Kinases/genetics , Cyclin-Dependent Kinases/metabolism , Female , Gene Expression , Humans , Immunophenotyping , Infant, Newborn , Ki-67 Antigen/genetics , Ki-67 Antigen/metabolism , Mesenchymal Stem Cells/metabolism , Multipotent Stem Cells/metabolism , Placenta/metabolism , Pregnancy , Primary Cell Culture , Proto-Oncogene Proteins c-bcl-2/genetics , Proto-Oncogene Proteins c-bcl-2/metabolism , Umbilical Cord/metabolismABSTRACT
On the territory of Kazakhstan there are uranium deposits, many ofwhich are in mothballed since times of perestroika. Often, the mines are flooded and represent a "time-delay bomb". Inside of mines various there are accumulated gases of both organic and inorganic nature, periodically thrown out and adversely affecting on the health of local populations. The aim of the study was the investigation of the state of the environment of Esilsky district of the Akmola region by common pollutants and chemicals. As the basic variable for the investigation of ambient air there was accepted the maximum one-time concentration of suspended substances, phenol, nitrogen dioxide, sulfur dioxide. The results were evaluated in relation to the MPC for the analyzed substance in the air according to maximal single MPC (MPCms) and daily average MPC (MPCda). The content of metals in the water was determined with the use of spectrophotometer PD-303S. Evaluation of the results was executed in relation of the MPC of substances in water, by means of the comparison with the requirements of Federal standards for drinking water, samples from drinking water sources. There were executed calculations of the overall index of water pollution (IWVgen), the index of water pollution by heavy metals (IWVhm). Chemical analysis of soil was carried out with the use of spectrophotometer PD- 303S (Japan), the photometer expert-003 "Ekoniks". Evaluation of the results was carried out with the respect to the MPC in the soil, the toxicity of all components. Summarizing soil pollution index was evaluated for metals contained in the soil at the level of more than or equal to 1 MAC. The settlement Krasnogorskiy and the village of Kalachi were found to be characterized by a low level of air pollution, increased rigidity of drinking water exceeded the maximum permissible concentrations of copper by 3.45 times and chloride by 1.17 times in the soil cover.
Subject(s)
Air Pollutants, Radioactive , Environmental Illness , Mining , Soil Pollutants, Radioactive , Uranium , Water Pollution, Radioactive , Air Pollutants, Radioactive/adverse effects , Air Pollutants, Radioactive/analysis , Environmental Exposure/analysis , Environmental Exposure/statistics & numerical data , Environmental Illness/chemically induced , Environmental Illness/epidemiology , Environmental Illness/prevention & control , Environmental Monitoring/methods , Environmental Monitoring/statistics & numerical data , Humans , Kazakhstan/epidemiology , Metals, Heavy/adverse effects , Metals, Heavy/analysis , Public Health/methods , Public Health/statistics & numerical data , Soil Pollutants, Radioactive/adverse effects , Soil Pollutants, Radioactive/analysis , Uranium/adverse effects , Uranium/chemistry , Waste Products/adverse effects , Waste Products/analysis , Waste Products/statistics & numerical data , Water Pollution, Radioactive/adverse effects , Water Pollution, Radioactive/analysisABSTRACT
Proliferative activity of mesenchymal stromal cells isolated from five sources (chorionic villi, Wharton's jelly, amnion, endometrium, and adipose tissue) was compared by flow cytometry and real-time PCR (by the content of mRNA of genes encoding of cell cycle regulators). Mesenchymal stromal cells derived from the endometrium demonstrated maximum stability and high proliferative potential.
Subject(s)
Adipose Tissue/cytology , Amnion/cytology , Chorionic Villi/growth & development , Endometrium/cytology , Mesenchymal Stem Cells/cytology , Wharton Jelly/cytology , Adult , Cell Differentiation , Cell Proliferation , Cells, Cultured , Female , Flow Cytometry , Humans , Real-Time Polymerase Chain Reaction , Young AdultABSTRACT
The problem of Mycobacterium tuberculosis virulence, together with drug resistance, is becoming key for the design of drugs with a new mechanism of action and the production of modern concepts and tuberculosis treatment schemes. The review describes gene complexes and their products, including mycolic acids and global regulatory systems at the level of transcriptional, translational, and post-translational modification, etc. The criteria for selection of virulence/pathogenicity factors that might be used for comparative genomic analysis of strains differing in the degree of virulence were recommended. The experimental approaches and test systems for an adequate estimation of the virulence degree of different strains of M. tuberculosis were analyzed.
Subject(s)
Bacterial Proteins , Gene Expression Regulation, Bacterial/physiology , Mycobacterium tuberculosis , Protein Processing, Post-Translational/physiology , Transcription, Genetic/physiology , Virulence Factors , Bacterial Proteins/biosynthesis , Bacterial Proteins/genetics , Humans , Mycobacterium tuberculosis/genetics , Mycobacterium tuberculosis/metabolism , Mycobacterium tuberculosis/pathogenicity , Virulence Factors/biosynthesis , Virulence Factors/geneticsABSTRACT
Morphometric analysis of changes in nucleolar organizer (NO), Balbiani rings (BR)--BR(B), BR(1G), BR(2G) and chromosome I arm B puff activities, and in chromosome compactness of Chironomus plumosus (Diptera) polytene chromosomes was carried out in acute period under separate and combined influence of atropine and pilocarpine. Supression effect of cholinotropic preparations mixture was revealed. Suppression of NO activity with atropine concentration increase in the mixture served as criterion of toxicity.
Subject(s)
Atropine/toxicity , Chironomidae/drug effects , Muscarinic Agonists/toxicity , Muscarinic Antagonists/toxicity , Nucleolus Organizer Region/drug effects , Pilocarpine/toxicity , Animals , Chironomidae/cytology , Chironomidae/genetics , Insect Proteins/drug effects , Larva/cytology , Larva/drug effects , Larva/genetics , Nucleolus Organizer Region/ultrastructureABSTRACT
The level of bone metabolites and the indices of calcium and phosphorus metabolism were measured in 20 women with climacteric syndrome in postmenopause and in 20 women in premenopause, who had normal bone density and did not differ in the modified risk factors of osteoporosis. In the climacteric women, the normal bone density was accompanied by the elevated levels of CrossLaps and osteocalcin. There was no difference in the concentrations of parathormone, calcium, and phosphorus. After 9-month non-hormonal correction of climacteric syndrome with Estrovel (Ecomir, Russia), the levels of CrossLaps reduced by 45% and that of osteocalcin became normal.
