[Vesiculation of erythrocytes during storage and connection of it with other processes in the cell]. / Vezikuliatsiia -eritrotsitov pri ikh khranenii i sviaz' ee s drugimi protsessami v kletke.

The activation energies of three processes (AT-depended vesiculation, release of potassium ions from erythrocytes and formation of methemoglobin) in erythrocytes incubated in phosphate buffer are measured in the range 4-37 degrees C. It is show that the Arrhenius plots for the first two processes have the same characteristics namely break at temperature around 18 degrees C, and Ea-58 kJ/mol at t > 18 degrees C. These data suggest that the velocity of ATP-depended vesiculation of erythrocytes is determined by the membrane permeability to monovalent cations. The appearance of methemoglobin in extracellular medium is characterized by a distinct value Ea(120 kJ/mol), although kinetics of this process is similar to vesiculation kinetics. Connection between considered processes and interrelation between ATP-depended vesiculation and spontaneous vesiculation are discussed.

[Vesiculation and change of erythrocyte membrane proteins in storage of preserved blood and erythrocyte mass at 4 degrees C]. / Vezikuliatsiia i izmenenie membrannykh belkov éritrotsitov v protsesse khraneniia konservirovannoi krovi i éritrotsitnoi massy pri 4 C.

Erythrocyte vesiculation and changes in membrane protein composition during storage of blood and red cell concentrates in solution "Glugicir" at 4 +/- 2 degrees C have been studied. It is shown that the main part of vesicles is shedding after 9 days of storage. This process corresponds to a decrease of protein 3 and to polyphase changes of membrane-bound hemoglobin as is shown by gel-electrophoresis. During 9-day storage, when erythrocyte ATP is constant and the vesicle shedding is small, an increase in membrane-bound hemoglobin and a decrease of spectrin in isolated membranes take place. The above changes are not the result of oxidative processes in membrane lipid bilayer or in hemoglobin. There was also protein destruction in membranes of stored erythrocytes. It is suggested that in stored blood two type of erythrocyte vesiculation take place: one is due to cell senescence, the other results from metabolic depletion.

[Change of state of erythrocyte membrane proteins during storage of preserved blood]. / Izmenenie sostoianiia membrannykh belkov éritrotsitov v protsesse khraneniia konservirovannoi krovi.

Erythrocyte vesiculation, kinetic parameters of tryptophane phosphorescence at room temperature and activity of erythrocyte membrane during storage of human blood at 4 +/- 2 C were studied. It is shown that before the erythrocyte vesiculation stage (10-15 days of storage) two types of changes in the structural state of the membrane proteins occur: 1) on the 1-5th days and 2) on the 5-10th days of blood storage.

[Changes in the structural-functional state of erythrocyte membranes treated with anion transport inhibitors]. / Izmenenie strukturno-funktsional'nogo sostoianiia membran éritrotsitov pod vozdeistviem ingibitorov transporta anionov.

Effect of 4,4-dyisotiocyanostilben-2,2-disulfonate (DIDS) and 1-ftor-2,4-dinitrobenzol (NDFB) on the rate of phosphate ion transport in erythrocytes, filtrability and thermal stability of erythrocytes and on the structural state of the erythrocyte membrane estimated by UV-fluorescence, PAAG--electrophoresis and measuring of the activity of membrane-bound acetylcholinesterase (AChE) has been studied. Unpenetrating anion transport inhibitor DIDS is shown to induce structural modifications of bands 3 of protein and AChE, while DNFB penetrating the membrane causes a significant reorganization of many membrane proteins (including spectrin) resulting in changes of transport and mechanical properties of erythrocytes.