Jasmonates Alleviate the Harm of High-Temperature Stress During Anthesis to Stigma Vitality of Photothermosensitive Genetic Male Sterile Rice Lines.

Using photothermosensitive genic male sterile (PTSGMS) rice (Oryza sativa L.) lines to produce hybrids can obtain great heterosis. However, PTSGMS rice lines exhibit low stigma vitality when high-temperature (HT) stress happens during anthesis. Jasmonates (JAs) are novel phytohormones and play vital roles in mediating biotic and abiotic stresses. Little is known, however, if and how JAs could alleviate the harm of HT stress during anthesis to the stigma vitality of PTSGMS lines. This study investigated the question. Two PTSGMS lines and one restorer line of rice were pot-grown and subjected to normal temperature and HT stress during anthesis. The stigma exertion rate, sigma fresh weight, stigma area, contents of JAs, hydrogen peroxide (H2O2), and ascorbic acid (AsA), activity of catalase in stigmas, and the number of pollens germinated on the stigma of PTSGMS lines were determined. The results showed that a rice line with higher JAs content in the stigma under HT stress showed lower H2O2 content, higher AsA content and catalase activity in stigmas, larger stigma area, heavier stigma fresh weight, more pollens germinated on the stigma, and higher fertilization and seed-setting and rates. Applying methyl JAs during anthesis to rice panicles decreased the accumulation of reactive oxygen species and enhanced stigma vitality, thereby increasing fertilization and seed-setting rates of the hybrids of PTSGMS rice lines under HT stress. The results demonstrate that JAs attenuate the injury of HT stress to the stigma vitality of PTSGMS rice lines through enhancing antioxidant ability.

Physiological mechanism underlying the effect of high temperature during anthesis on spikelet-opening of photo-thermo-sensitive genic male sterile rice lines.

Decrease in the grain yield resulted from a low percentage of opened spikelets under high temperature (HT) during anthesis is a serious problem in the seed production of photo-thermo-sensitive genic male sterile (PTGMS) rice (Oryza sativa L.) lines, and the mechanism is little understood. Elucidating the physiological mechanism underlying the effect of HT during anthesis on spikelet-opening of PTGMS lines would have great significance in exploring the effective way to mitigate the adverse effect of HT. In this study, two PTGMS lines and one restorer line of rice were used and were subjected to normal temperature (NT) and HT treatments. The results showed that, compared with NT, HT significantly decreased the percentage of opened spikelets, fertilization percentage and seed-setting by significantly increasing the percentage of wrapped spikelets and reducing the spikelet-opening angle, length of spikelet-opening time. The HT significantly decreased the contents of soluble sugars, jasmonic acid (JA) and methyl jasmonate (MeJA) in the lodicules before and at glume-opening, which were significantly correlated with and accounts for the low percentage of opened spikelets under HT for rice, especially for the PTGMS lines.