ABSTRACT
A novel actinomycete, designated strain KLBMP 1256(T), was isolated from a surface-sterilized stem of the oil-seed plant Jatropha curcas L. collected from Sichuan Province, south-west China, and was characterized to determine its taxonomic position. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the isolate was closely related to members of the genus Nocardia in the family Nocardiaceae, being most closely related to Nocardia callitridis CAP 290(T) (98.4 % similarity) and Nocardia nova JCM 6044(T) (97.5 %). Levels of 16S rRNA gene sequence similarity between strain KLBMP 1256(T) and the type strains of other recognized species of the genus Nocardia were less than 97 %. Chemotaxonomic data supported the affiliation of the new isolate to the genus Nocardia. However, the novel strain could be distinguished from its closest phylogenetic neighbour, N. callitridis CAP 290(T), by a range of phenotypic properties. The combination of low DNA-DNA relatedness values and phenotypic differences from N. callitridis CAP 290(T) indicated that strain KLBMP 1256(T) represents a novel species of the genus Nocardia, for which the name Nocardia endophytica sp. nov. is proposed. The type strain is KLBMP 1256(T) (â= KCTC 19777(T) â= CCTCC AA 2010004(T)).
Subject(s)
Jatropha/microbiology , Nocardia/classification , Nocardia/isolation & purification , DNA, Bacterial/genetics , DNA, Ribosomal/genetics , Jatropha/physiology , Molecular Sequence Data , Nocardia/genetics , Nocardia/physiology , Phylogeny , RNA, Ribosomal, 16S/genetics , SymbiosisABSTRACT
A novel isolate, designated strain KLBMP 1115(T) was isolated from the surface-sterilized root of oil-seed plant Jatropha curcas L. collected from Sichuan Province, south-west China. Characterization of the isolate was based on a polyphasic approach. 16S rRNA gene sequence analysis indicated that strain KLBMP 1115(T) belongs to the phylogenetic cluster of the genus Pseudonocardia and was most closely related to Pseudonocardia adelaidensis EUM 221(T) (98.9%) and Pseudonocardia zijingensis DSM 44774(T) (98.6%), whereas the DNA-DNA relatedness values between strain KLBMP 1115(T) and the two type strains were 47.3 and 39.7%, respectively. Levels of lower similarities to the type strains of other recognized Pseudonocardia species ranged from 94.4 to 98.4%. The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. The predominant respiratory quinone was MK-8(H(4)). The major fatty acids of strain KLBMP 1115(T) was iso-C(16:0). The chemotaxonomic properties of strain KLBMP 1115(T) were consistent with those shared by members of the genus Pseudonocardia. On the basis of the phenotypic features and the DNA-DNA hybridization data, strain KLBMP 1115(T) represents a novel species of the genus Pseudonocardia, for which the name Pseudonocardia sichuanensis sp. nov. is proposed. The type strain is KLBMP 1115(T) (=KCTC 19781(T) = CCTCC AA 2010002(T)).
