Impact of Escherichia coli K12 and O18:K1 on human platelets: Differential effects on platelet activation, RNAs and proteins.

Blood platelets can interact with bacteria, possibly leading to platelet activation, cytokine and microparticle release and immune signalling. Besides, bacteria can also affect the platelet RNA content. We investigated the impact of non-pathogenic K12 and pathogenic O18:K1 Escherichia (E.) coli strains on platelet activation, RNA expression patterns, and selected proteins. Depending on bacteria concentration, contact of platelets with E. coli K12 lead to an increase of P-selectin (24-51.3%), CD63 (15.9-24.3%), PAC-1 (3.8-14.9%) and bound fibrinogen (22.4-39%) on the surface. E. coli O18:K1 did not affect these markers. Sequencing analysis of total RNA showed that E. coli K12 caused a significant concentration change of 103 spliced mRNAs, of which 74 decreased. For the RNAs of HMBS (logFC = +5.73), ATP2C1 (logFC = -3.13) and LRCH4 (logFC = -4.07) changes were detectable by thromboSeq and Tuxedo pipelines. By Western blot we observed the conversion of HMBS protein from a 47 kDA to 40 kDa product by E. coli K12, O18:K1 and by purified lipopolysaccharide. While ATP2C1 protein was released from platelets, E. coli either reduced the secretion or broke down the released protein making it undetectable by antibodies. Our results demonstrate that different E. coli strains influence activation, RNA and protein levels differently which may affect platelet-bacteria crosstalk.

Migraine is a neuronal disease.

Migraine is a common, paroxysmal, highly disabling primary headache disorder with a genetic background. The primary cause and the origin of migraine attacks are enigmatic. Numerous clinical and experimental results suggest that activation of the trigeminal system (TS) is crucial in its pathogenesis, but the primary cause of this activation is not fully understood. Since activation of the peripheral and central arms of the TS might be related to cortical spreading depression and to the activity of distinct brainstem nuclei (e.g. the periaqueductal grey), we conclude that migraine can be explained as an altered function of the neuronal elements of the TS, the brainstem, and the cortex, the centre of this process comprising activation of the TS. In light of our findings and the literature data, therefore, we can assume that migraine is mainly a neuronal disease.

[Psychotherapeutic possibilities in patients with definitive movement disorders]. / Psychotherapeutische Möglichkeiten bei definitiv Bewegungsgeschädigten.

Since traumas leading to severe and permanent physical handicaps affect the whole personality, the psychological approach plays a major role in the rehabilitation of patients with locomotory handicaps owing to injury. The harmful effects become apparently more easily on account of the disturbed personality development of the cripple, and the consequent mental changes complicate his or her reintegration into society. Rehabilitation measures intended to restore, as far as possible, the physical functions can only yield satisfactory results if they prepare the whole personality of the cripple--as a combined psychological and somatic entity--for life of a different quality.

[Psychological problems in children living with parents suffering from traumatic lesions of the vertebral column (author's transl)]. / Psychologische Probleme bei Kindern von Eltern mit Wirbelsäulen-und Rückenmark-Verletzungen

After trauma of the vertebral column and the neuromuscular consequences in one of the parents, children must experience many changes in the life of the family. They tend to react by psychosomatical symptoms as f.i. Anorexy, Colitis mucosa, Enuresis nocturna, Emesis a.s.o. Child guidance in such a case is urgently needed, as the author shows in two case reports.